LOCUS       62056_10630        5033 bp DNA     circular SYN 17-JUL-2019
DEFINITION  Cloning vector pFA-myc-LAL, complete sequence.
ACCESSION   MK652125
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F, 
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     A new toolkit for gene tagging in Candida albicans containing 
            recyclable markers
  JOURNAL   PLoS ONE (2019) In press
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F, 
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Instituto de Biologia Funcional y Genomica 
            (IBFG), Consejo Superior de Investigaciones Cientificas, Zacarias 
            Gonzalez 2, Salamanca, Salamanca 37007, Spain
REFERENCE   3  (bases 1 to 5033)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE 
            (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-MAR-2019) Instituto de Biologia Funcional y Genomica (IBFG), 
            Consejo Superior de Investigaciones Cientificas, Zacarias Gonzalez 
            2, Salamanca, Salamanca 37007, Spain"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 13
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             54..83
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     regulatory      222..477
                     /note="Transcriptional termination from Saccharomyces
                     cerevisiae URA3 gene"
                     /regulatory_class="terminator"
     primer_bind     487..503
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    505..538
                     /label=loxP
                     /note="loxP"
     gene            539..2539
                     /gene="ARG4"
                     /label=ARG4
     CDS             1005..2411
                     /codon_start=1
                     /transl_table=11
                     /gene="ARG4"
                     /product="ARG4"
                     /label=ARG4
                     /note="argininosuccinate lyase; derived from Candida
                     albicans ARG4"
                     /protein_id="QDK59778.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELHLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF
                     DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG
                     ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDAIGGTAKSAVLK
                     QLENLKSILS"
     protein_bind    complement(2540..2573)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(2671..2689)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2947..3535)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3709..4566)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4567..4671)
                     /label=AmpR promoter
     promoter        5017..5033
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc ggggaacaga
       61 agcttatatc cgaagaagac ctcggagagc aaaagctcat ttcagaagag gatctaggcg
      121 aacagaaact aatctcggag gaggacctcg gtgaacaaaa gcttatctct gaggaagatc
      181 ttggcgagca gaagctcata tcagaggaag acctagggta gggcgcgcca ttataagtaa
      241 atgcatgtat actaaactca caaattagag cttcaattta attatatcag ttattacccg
      301 ggaatctcgg tcgtaatgat ttctataatg acgaaaaaaa aaaaattgga aagaaaaagc
      361 ttcatggcct ttataaaaag gaactatcca atacctcgcc agaaccaagt aacagtattt
      421 tacggggcac aaatcaagaa caataagaca ggactgtaaa gatggacgca tagatccggc
      481 ggccgctcta gaactagtgg atccataact tcgtatagca tacattatac gaagttatag
      541 atccgtaccc ccctttagta agatttttca agagtagtct caaataaaca gaatgaagga
      601 ttgaaggttt tattcaataa ataattggac agaaagttat tgtacagttt taacaacaac
      661 aatttcaaaa tttcaattta tgacatattg accgacataa ttcagtcatt tctaatattc
      721 aaataacaag acaccttgta taaatgaatc cacaatcgta tatgaacaac caaattttat
      781 gactaacttt gggtcgtgta cttttgacga ttgagtcatt tttaccaatt aggtaattga
      841 attttgttaa tttgtcatta accgcgctgg ccatcccaac aataacaaca atcaatttaa
      901 aaacttgaca aggcaaaaaa aaaaaaattt tatctttgaa aaatttttct tccttttctt
      961 cttctgttct ttacataaca gctatcaaga ataatcaatt aataatgtca caacaacaag
     1021 ataaacaacc tagtgaaaat aaattatggg gtggtcgttt caccggtgcc actgatccat
     1081 tgatggattt atataatgct tcattacctt atgataaagt tatgtatgat gctgatttaa
     1141 ctggtactaa agtatacaca caaggattaa acaaattagg attaattacc actgaagaat
     1201 tgcatttgat tcatcaagga ttagaacaaa tcagacaaga atggcatgat aataaattca
     1261 ttattaaggc aggtgatgag gatattcata ctgctaatga acgtcgatta ggagaaatca
     1321 ttgggaaaaa catttctggt aaagttcaca ctggtagatc aagaaatgat caagttgcta
     1381 ccgatatgag aattttcgtt cgtgaatcat tacttaattt aagtaaaatt ttacatcaat
     1441 tcatcacggc aattcttgaa cgagcacata aagaaatcga tgtattaatg cctggttata
     1501 ctcatttaca aaaagctcaa cctattagat gggcccattg gttaagttca tatgcaactt
     1561 atttcaccga agattataaa cgtttacaag aaattataac tcgtgttaac caatctccat
     1621 taggatctgg tgctttagca ggtcatcctt atggtattga tcgtgaattt ttagctaaag
     1681 gattaggatt tgatggtgtc attgggaatt ctttaactgc agtttcagat cgtgattttg
     1741 ttgttgaaag tttattttgg agtacattat ttatgaatca tatttctcga ttctcagaag
     1801 atctaattat ttattctagt ggtgaatttg ggttcataaa attagctgat gcttattcta
     1861 ctggttcttc attgatgcca caaaagaaaa accctgactc attagaatta ttacgtggga
     1921 aaagtggtag agtatttggt cagttatctg gatttttaat gtcgatcaaa tctattccat
     1981 ctacttataa taaagatatg caagaggata aagaaccatt atttgatgca ttgactacag
     2041 tggaacattc aattttaatt gctactggtg tgatttctac tttactgatt gataaacaaa
     2101 atatggaaaa agctttaact atggatatgt tggctactga tttagctgat tatttagtta
     2161 gaaaaggagt tccatttaga gaaactcatc atatatctgg tgaatgtgtt agaaaagctg
     2221 aagaagaaaa attatctgga attgatcaat tatcttttga acaatttcaa caaattgatt
     2281 ctcgttttga aaaagacgtt atggaaactt ttgattttga agctagtgtg gaaagaagag
     2341 atgctattgg tggtactgct aaaagtgccg ttttaaaaca attagaaaat ttaaaatcaa
     2401 ttttaagtta atttataaat agtcatataa taatcacagt attgtttaac ttacataaaa
     2461 tataatcaat taacacagag ataccttgta ctccaatact agcattccaa ccagcatttc
     2521 tatacctgct agcccatgga taacttcgta tagcatacat tatacgaagt tattagggat
     2581 aacagggtaa tctgcaggaa aaacgagctc gaattcatcg atgatatcag atccactagt
     2641 ggcctatgcg gccgcggatc tgccggtctc cctatagtga gtcgtattaa tttcgataag
     2701 ccaggttaac ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     2761 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2821 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2881 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2941 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3001 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3061 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3121 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3181 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3241 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3301 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3361 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     3421 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3481 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3541 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3601 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3661 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     3721 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     3781 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3841 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3901 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3961 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     4021 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     4081 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     4141 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     4201 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     4261 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     4321 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     4381 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     4441 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     4501 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     4561 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4621 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4681 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     4741 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     4801 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     4861 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     4921 atcagagcag attgtactga gagtgcacca tatggacata ttgtcgttag aacgcggcta
     4981 caattaatac ataaccttat gtatcataca catacgattt aggtgacact ata
//
