LOCUS       V016435                 4984 bp    DNA     circular SYN 17-JUL-2019
DEFINITION  Exported.
ACCESSION   V016435
VERSION     V016435
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     A new toolkit for gene tagging in Candida albicans containing
            recyclable markers
  JOURNAL   PLoS ONE (2019) In press
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Instituto de Biologia Funcional y Genomica
            (IBFG), Consejo Superior de Investigaciones Cientificas, Zacarias
            Gonzalez 2, Salamanca, Salamanca 37007, Spain
REFERENCE   3  (bases 1 to 4984)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 13
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE
            (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAR-2019) Instituto de Biologia Funcional y Genomica (IBFG),
            Consejo Superior de Investigaciones Cientificas, Zacarias Gonzalez
            2, Salamanca, Salamanca 37007, Spain"
FEATURES             Location/Qualifiers
     source          1..4984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             65..769
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     regulatory      773..1028
                     /note="Transcriptional termination from Saccharomyces
                     cerevisiae URA3 gene"
                     /regulatory_class="terminator"
     primer_bind     1038..1054
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    1068..1101
                     /label="loxP"
                     /note="loxP"
     CDS             1531..2340
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     protein_bind    complement(2491..2524)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(2622..2640)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2898..3486)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3660..4517)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4518..4622)
                     /label="AmpR promoter"
     promoter        4968..4984
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acgggtgctg gcgcaggtgc
       61 ttctgtttca aaaggtgaag aagataatat ggctattatt aaagaattta tgagatttaa
      121 agttcatatg gaaggttcag ttaatggtca tgaatttgaa attgaaggtg aaggtgaagg
      181 tagaccatat gaaggtactc aaactgctaa attgaaagtt actaaaggtg gttcattacc
      241 atttgcttgg gatattttgt caccacaatt tatgtatggt tcaaaagctt atgttaaaca
      301 tccagctgat attccagatt atttaaaatt gtcatttcca gaaggtttta aatgggaaag
      361 agttatgaat tttgaagatg gtggtgttgt tactgttact caagattcat cattacaaga
      421 tggtgaattt atttataagg ttaaattgag aggtactaat tttccatcag atggtccagt
      481 tatgcaaaaa aaaactatgg gttgggaagc ttcatcagaa agaatgtatc cagaagatgg
      541 tgctttaaaa ggtgaaatta aacaaagatt gaaattaaaa gatggtggtc attatgatgc
      601 tgaagttaaa actacttata aagctaaaaa accagttcaa ttaccaggtg cttataatgt
      661 taatattaaa ttggatatta cttcacataa tgaagattat actattgttg aacaatatga
      721 aagagctgaa ggtagacatt caactggtgg tatggatgaa ttatataaat aaggcgcgcc
      781 attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt aattatatca
      841 gttattaccc gggaatctcg gtcgtaatga tttctataat gacgaaaaaa aaaaaattgg
      901 aaagaaaaag cttcatggcc tttataaaaa ggaactatcc aatacctcgc cagaaccaag
      961 taacagtatt ttacggggca caaatcaaga acaataagac aggactgtaa agatggacgc
     1021 atagatccgg cggccgctct agaactagtg gatccagatc ttgatccata acttcgtata
     1081 gcatacatta tacgaagtta tagatccagt actaatagga attgatttgg atggtataaa
     1141 cggaaacaaa aaaaagagct ggtactactt tctttaaaat tattttatta tttgatttta
     1201 tttaatagta tatattatat tttgaacgta gattattttg ttgaaagttg ctgtagtgcc
     1261 attgattcgt aacactaatt ctgtattagt cattcctctt gtttgatagt atccaaaaaa
     1321 acggctattt ttttgcaatc ttatttcctg catattatac agataacata atgaaagaaa
     1381 aaatcttttt ttttgttctt caatgatgat ttcaaccatt cttttaaaca ttgatcaatt
     1441 cctgagcaac aaccccatac acactggttt atatatcgcc ccttttacag ttgaagaaag
     1501 aaatagaaat agaaatagca aacaaaagat atgacagtca acactaagac ctatagtgag
     1561 agagcagaaa ctcatgcctc accagtagca caacgattat ttcgattaat ggaactgaag
     1621 aaaaccaatt tatgtgcatc aattgatgtt gataccacta aggaattcct tgaattaatt
     1681 gataaattgg gtccttatgt atgcttaatc aagactcata ttgatataat caatgatttt
     1741 tcctatgaat ccactattga accattatta gaactttcac gtaaacatca atttatgatt
     1801 tttgaagata gaaaatttgc tgatattggt aataccgtga agaaacaata tattggtgga
     1861 gtttataaaa ttagtagttg ggcagatatt actaatgctc atggtgtcac tgggaatgga
     1921 gtagttgaag gattaaaaca gggagctaaa gaaaccacca ccaaccaaga gccaagaggg
     1981 ttattgatgt tagctgaatt atcatcagtg ggatcattag catatggaga atattctcaa
     2041 aaaactgttg aaattgctaa atccgataag gaatttgtta ttggatttat tgcccaacgt
     2101 gatatgggtg gacaagaaga aggatttgat tggcttatta tgacacctgg agttggatta
     2161 gatgataaag gtgatggatt aggacaacaa tatagaactg ttgatgaagt tgttagcact
     2221 ggaactgata ttatcattgt tggtagagga ttgtttggta aaggaagaga tccagatatt
     2281 gaaggtaaaa ggtatagaga tgctggttgg aatgcttatt tgaaaaaaac tggccaatta
     2341 taaatgtgaa gggggagatt ttcactttat tagatttgta tatatgtata ataaataaat
     2401 aaataagtta aataaataat taaataaggg tggtaattat tactatttac aatcaaaggt
     2461 ggtccttcta gaagatctgc tagcccatgg ataacttcgt atagcataca ttatacgaag
     2521 ttattaggga taacagggta atctgcagga aaaacgagct cgaattcatc gatgatatca
     2581 gatccactag tggcctatgc ggccgcggat ctgccggtct ccctatagtg agtcgtatta
     2641 atttcgataa gccaggttaa cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     2701 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     2761 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2821 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2881 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2941 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3001 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3061 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     3121 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3181 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3241 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3301 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     3361 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3421 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3481 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3541 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3601 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     3661 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3721 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3781 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3841 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3901 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3961 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4021 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4081 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4141 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4201 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4261 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     4321 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     4381 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     4441 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     4501 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     4561 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     4621 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     4681 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     4741 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     4801 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     4861 aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat attgtcgtta
     4921 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
     4981 tata
//
