LOCUS       V015294                 4878 bp    DNA     circular SYN 17-JUL-2019
DEFINITION  Exported.
ACCESSION   V015294
VERSION     V015294
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     A new toolkit for gene tagging in Candida albicans containing
            recyclable markers
  JOURNAL   PLoS ONE (2019) In press
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Instituto de Biologia Funcional y Genomica
            (IBFG), Consejo Superior de Investigaciones Cientificas, Zacarias
            Gonzalez 2, Salamanca, Salamanca 37007, Spain
REFERENCE   3  (bases 1 to 4878)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 13
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE
            (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAR-2019) Instituto de Biologia Funcional y Genomica (IBFG),
            Consejo Superior de Investigaciones Cientificas, Zacarias Gonzalez
            2, Salamanca, Salamanca 37007, Spain"
FEATURES             Location/Qualifiers
     source          1..4878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             65..769
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     regulatory      773..1028
                     /note="Transcriptional termination from Saccharomyces
                     cerevisiae URA3 gene"
                     /regulatory_class="terminator"
     primer_bind     1038..1054
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    1068..1101
                     /label="loxP"
                     /note="loxP"
     CDS             1449..2342
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     protein_bind    complement(2385..2418)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(2516..2534)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2792..3380)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3554..4411)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4412..4516)
                     /label="AmpR promoter"
     promoter        4862..4878
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acgggtgctg gcgcaggtgc
       61 ttctgtttca aaaggtgaag aagataatat ggctattatt aaagaattta tgagatttaa
      121 agttcatatg gaaggttcag ttaatggtca tgaatttgaa attgaaggtg aaggtgaagg
      181 tagaccatat gaaggtactc aaactgctaa attgaaagtt actaaaggtg gttcattacc
      241 atttgcttgg gatattttgt caccacaatt tatgtatggt tcaaaagctt atgttaaaca
      301 tccagctgat attccagatt atttaaaatt gtcatttcca gaaggtttta aatgggaaag
      361 agttatgaat tttgaagatg gtggtgttgt tactgttact caagattcat cattacaaga
      421 tggtgaattt atttataagg ttaaattgag aggtactaat tttccatcag atggtccagt
      481 tatgcaaaaa aaaactatgg gttgggaagc ttcatcagaa agaatgtatc cagaagatgg
      541 tgctttaaaa ggtgaaatta aacaaagatt gaaattaaaa gatggtggtc attatgatgc
      601 tgaagttaaa actacttata aagctaaaaa accagttcaa ttaccaggtg cttataatgt
      661 taatattaaa ttggatatta cttcacataa tgaagattat actattgttg aacaatatga
      721 aagagctgaa ggtagacatt caactggtgg tatggatgaa ttatataaat aaggcgcgcc
      781 attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt aattatatca
      841 gttattaccc gggaatctcg gtcgtaatga tttctataat gacgaaaaaa aaaaaattgg
      901 aaagaaaaag cttcatggcc tttataaaaa ggaactatcc aatacctcgc cagaaccaag
      961 taacagtatt ttacggggca caaatcaaga acaataagac aggactgtaa agatggacgc
     1021 atagatccgg cggccgctct agaactagtg gatccccatg gtgatccata acttcgtata
     1081 gcatacatta tacgaagtta tagatccggt acctggagga tgaggagaca gaagttagta
     1141 gtaacaattt ggggacagaa gaggatacag tgacatcaga ggaagaagac agcgaaggta
     1201 tagtcaaaaa gcaataggac agcaagacaa tttatttatt taatagtttt ttataaatgt
     1261 aaaatctatc acaacatgtg tacttaattt tctgtggtag gggaggccat ttcgttgaaa
     1321 cgtgtcgcga gcggttgtga gaattgactc attttgatta ttacgcaacc attgaaattt
     1381 tttatttttt tttggtgaag atttttccca cacaacttct tcttttactt aaccaccaac
     1441 taccgataat ggatttagtc aatcatttac cagaccgttt gttatttgct gttcctaaaa
     1501 agggcagatt atacgaaaaa tgctgtaact tattgagtgg tgccgatata cagtttagaa
     1561 gatctaatag attagatata gcactttcta caaacttgcc aattgcatta atcttcttgc
     1621 ctgcagctga tatcccagtt ttcgttggag aaggtaattg tgacttgggt ataactgggt
     1681 tagaccaaat caaagaagct gaacaattcg acaacatcga ggacttgttg gatttgaaat
     1741 ttggttcatg caaattgcag atccaagttc cagcagatgg cgagtacgaa aagccagaac
     1801 agcttgttgg aaagaaaatt gtgtcttcat ttacaaaatt gagtaccgac tatttcaaac
     1861 aattgtcaga caaacctact aatatcagat atgtcggtgg ttccgttgag gcttcttgtg
     1921 ccttgggtgt tgctgatgct attgtcgatt tggttgaaag tggtgaaact atgaaagcag
     1981 ctgggttaaa ggccatagaa accatattgg agacttcggc tcatttgatt tcgtcaaaga
     2041 aaagtaaatt cccagaaatg gtcaatataa tcgtccaaag acttcaaggt gttttagctg
     2101 cccaggaata tgtcttgtgt aactacaatg ccccaaaatc catccaagca aaatgtttaa
     2161 ccattactcc aggcagaagg gccgctactg tctctacttt ggacaaacac agcgacgacg
     2221 aagaggactg ggttgccatc tcatccatgg ttaatagaaa ggaaatcggt aatgtaatgg
     2281 acgaattgaa gaaagctggt gcaaccgata tattggtact cgagatatca aattgtagag
     2341 tttaaaagaa gtgatagttt ctcataaata ttgctagccc atggataact tcgtatagca
     2401 tacattatac gaagttatta gggataacag ggtaatctgc aggaaaaacg agctcgaatt
     2461 catcgatgat atcagatcca ctagtggcct atgcggccgc ggatctgccg gtctccctat
     2521 agtgagtcgt attaatttcg ataagccagg ttaacctgca ttaatgaatc ggccaacgcg
     2581 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     2641 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     2701 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     2761 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     2821 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2881 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2941 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     3001 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3061 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3121 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3181 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     3241 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     3301 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     3361 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     3421 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     3481 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     3541 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3601 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     3661 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     3721 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     3781 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     3841 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     3901 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3961 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     4021 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     4081 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     4141 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     4201 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     4261 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     4321 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     4381 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     4441 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     4501 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     4561 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     4621 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     4681 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     4741 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgg
     4801 acatattgtc gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac
     4861 gatttaggtg acactata
//
