LOCUS       62056_10605        5584 bp DNA     circular SYN 17-JUL-2019
DEFINITION  Cloning vector pFA-mCherry-LAL, complete sequence.
ACCESSION   MK652115
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F, 
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     A new toolkit for gene tagging in Candida albicans containing 
            recyclable markers
  JOURNAL   PLoS ONE (2019) In press
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F, 
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Instituto de Biologia Funcional y Genomica 
            (IBFG), Consejo Superior de Investigaciones Cientificas, Zacarias 
            Gonzalez 2, Salamanca, Salamanca 37007, Spain
REFERENCE   3  (bases 1 to 5584)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE 
            (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-MAR-2019) Instituto de Biologia Funcional y Genomica (IBFG), 
            Consejo Superior de Investigaciones Cientificas, Zacarias Gonzalez 
            2, Salamanca, Salamanca 37007, Spain"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 13
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             65..769
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     regulatory      773..1028
                     /note="Transcriptional termination from Saccharomyces
                     cerevisiae URA3 gene"
                     /regulatory_class="terminator"
     primer_bind     1038..1054
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1056..1089
                     /label=loxP
                     /note="loxP"
     gene            1090..3090
                     /gene="ARG4"
                     /label=ARG4
     CDS             1556..2962
                     /codon_start=1
                     /transl_table=11
                     /gene="ARG4"
                     /product="ARG4"
                     /label=ARG4
                     /note="argininosuccinate lyase; derived from Candida
                     albicans ARG4"
                     /protein_id="QDK59754.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELHLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF
                     DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG
                     ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDAIGGTAKSAVLK
                     QLENLKSILS"
     protein_bind    complement(3091..3124)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(3222..3240)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3498..4086)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4260..5117)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5118..5222)
                     /label=AmpR promoter
     promoter        5568..5584
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acgggtgctg gcgcaggtgc
       61 ttctgtttca aaaggtgaag aagataatat ggctattatt aaagaattta tgagatttaa
      121 agttcatatg gaaggttcag ttaatggtca tgaatttgaa attgaaggtg aaggtgaagg
      181 tagaccatat gaaggtactc aaactgctaa attgaaagtt actaaaggtg gttcattacc
      241 atttgcttgg gatattttgt caccacaatt tatgtatggt tcaaaagctt atgttaaaca
      301 tccagctgat attccagatt atttaaaatt gtcatttcca gaaggtttta aatgggaaag
      361 agttatgaat tttgaagatg gtggtgttgt tactgttact caagattcat cattacaaga
      421 tggtgaattt atttataagg ttaaattgag aggtactaat tttccatcag atggtccagt
      481 tatgcaaaaa aaaactatgg gttgggaagc ttcatcagaa agaatgtatc cagaagatgg
      541 tgctttaaaa ggtgaaatta aacaaagatt gaaattaaaa gatggtggtc attatgatgc
      601 tgaagttaaa actacttata aagctaaaaa accagttcaa ttaccaggtg cttataatgt
      661 taatattaaa ttggatatta cttcacataa tgaagattat actattgttg aacaatatga
      721 aagagctgaa ggtagacatt caactggtgg tatggatgaa ttatataaat aaggcgcgcc
      781 attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt aattatatca
      841 gttattaccc gggaatctcg gtcgtaatga tttctataat gacgaaaaaa aaaaaattgg
      901 aaagaaaaag cttcatggcc tttataaaaa ggaactatcc aatacctcgc cagaaccaag
      961 taacagtatt ttacggggca caaatcaaga acaataagac aggactgtaa agatggacgc
     1021 atagatccgg cggccgctct agaactagtg gatccataac ttcgtatagc atacattata
     1081 cgaagttata gatccgtacc cccctttagt aagatttttc aagagtagtc tcaaataaac
     1141 agaatgaagg attgaaggtt ttattcaata aataattgga cagaaagtta ttgtacagtt
     1201 ttaacaacaa caatttcaaa atttcaattt atgacatatt gaccgacata attcagtcat
     1261 ttctaatatt caaataacaa gacaccttgt ataaatgaat ccacaatcgt atatgaacaa
     1321 ccaaatttta tgactaactt tgggtcgtgt acttttgacg attgagtcat ttttaccaat
     1381 taggtaattg aattttgtta atttgtcatt aaccgcgctg gccatcccaa caataacaac
     1441 aatcaattta aaaacttgac aaggcaaaaa aaaaaaaatt ttatctttga aaaatttttc
     1501 ttccttttct tcttctgttc tttacataac agctatcaag aataatcaat taataatgtc
     1561 acaacaacaa gataaacaac ctagtgaaaa taaattatgg ggtggtcgtt tcaccggtgc
     1621 cactgatcca ttgatggatt tatataatgc ttcattacct tatgataaag ttatgtatga
     1681 tgctgattta actggtacta aagtatacac acaaggatta aacaaattag gattaattac
     1741 cactgaagaa ttgcatttga ttcatcaagg attagaacaa atcagacaag aatggcatga
     1801 taataaattc attattaagg caggtgatga ggatattcat actgctaatg aacgtcgatt
     1861 aggagaaatc attgggaaaa acatttctgg taaagttcac actggtagat caagaaatga
     1921 tcaagttgct accgatatga gaattttcgt tcgtgaatca ttacttaatt taagtaaaat
     1981 tttacatcaa ttcatcacgg caattcttga acgagcacat aaagaaatcg atgtattaat
     2041 gcctggttat actcatttac aaaaagctca acctattaga tgggcccatt ggttaagttc
     2101 atatgcaact tatttcaccg aagattataa acgtttacaa gaaattataa ctcgtgttaa
     2161 ccaatctcca ttaggatctg gtgctttagc aggtcatcct tatggtattg atcgtgaatt
     2221 tttagctaaa ggattaggat ttgatggtgt cattgggaat tctttaactg cagtttcaga
     2281 tcgtgatttt gttgttgaaa gtttattttg gagtacatta tttatgaatc atatttctcg
     2341 attctcagaa gatctaatta tttattctag tggtgaattt gggttcataa aattagctga
     2401 tgcttattct actggttctt cattgatgcc acaaaagaaa aaccctgact cattagaatt
     2461 attacgtggg aaaagtggta gagtatttgg tcagttatct ggatttttaa tgtcgatcaa
     2521 atctattcca tctacttata ataaagatat gcaagaggat aaagaaccat tatttgatgc
     2581 attgactaca gtggaacatt caattttaat tgctactggt gtgatttcta ctttactgat
     2641 tgataaacaa aatatggaaa aagctttaac tatggatatg ttggctactg atttagctga
     2701 ttatttagtt agaaaaggag ttccatttag agaaactcat catatatctg gtgaatgtgt
     2761 tagaaaagct gaagaagaaa aattatctgg aattgatcaa ttatcttttg aacaatttca
     2821 acaaattgat tctcgttttg aaaaagacgt tatggaaact tttgattttg aagctagtgt
     2881 ggaaagaaga gatgctattg gtggtactgc taaaagtgcc gttttaaaac aattagaaaa
     2941 tttaaaatca attttaagtt aatttataaa tagtcatata ataatcacag tattgtttaa
     3001 cttacataaa atataatcaa ttaacacaga gataccttgt actccaatac tagcattcca
     3061 accagcattt ctatacctgc tagcccatgg ataacttcgt atagcataca ttatacgaag
     3121 ttattaggga taacagggta atctgcagga aaaacgagct cgaattcatc gatgatatca
     3181 gatccactag tggcctatgc ggccgcggat ctgccggtct ccctatagtg agtcgtatta
     3241 atttcgataa gccaggttaa cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3301 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3361 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3421 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3481 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3541 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3601 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3661 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     3721 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3781 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3841 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3901 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     3961 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4021 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4081 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4141 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4201 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4261 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4321 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4381 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     4441 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4501 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4561 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4621 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4681 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4741 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4801 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4861 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     4921 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     4981 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5041 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5101 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5161 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5221 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     5281 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     5341 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     5401 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     5461 aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat attgtcgtta
     5521 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
     5581 tata
//
