LOCUS       V006376                 4824 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006376
VERSION     V006376
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Epp E, Nazarova E, Douglas LM, Konopka JB, Vogel J, Whiteway M.
  TITLE     Actin dynamics and endocytosis in the absence of Arp2/3 in Candida
            albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Epp E, Nazarova E, Douglas LM, Konopka JB, Vogel J, Whiteway M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2011) Biology, McGill University, 1205 Docteur
            Penfield, Montreal, Quebec H3A 1B1, Canada
REFERENCE   3  (bases 1 to 4824)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4824)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-MAY-2011) Biology, McGill University, 1205 Docteur Penfield,
            Montreal, Quebec H3A 1B1, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             58..786
                     /label="TagRFP-T"
                     /note="photostable variant of TagRFP (Shaner et al., 2008)"
     regulatory      792..1045
                     /label="Saccharomyces cerevisiae URA3 terminator"
                     /note="Saccharomyces cerevisiae URA3 terminator"
                     /regulatory_class="terminator"
     CDS             1448..2257
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     promoter        complement(2462..2480)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2738..3326)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3500..4357)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4358..4462)
                     /label="AmpR promoter"
     promoter        4808..4824
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcagggtg ctggcgcagg tgcttcggta
       61 tctaagggtg aagaattgat taaggaaaac atgcacatga agttgtatat ggaaggtact
      121 gtaaataacc accattttaa gtgtacctct gaaggtgaag gtaaaccata tgaaggtaca
      181 caaaccatga gaattaaagt tgtagaaggt ggtccattgc cttttgcctt cgatatctta
      241 gctacatctt ttatgtacgg ttcaagaacc ttcatcaatc atactcaagg tataccagat
      301 ttctttaagc aatcattccc tgaaggtttc acttgggaaa gagtcactac atatgaagac
      361 ggtggtgttt tgactgccac acaagataca tccttgcaag acggttgttt aatctataac
      421 gttaagatca gaggtgtaaa cttcccaagt aacggtcctg taatgcaaaa gaaaactttg
      481 ggttgggaag caaacaccga aatgttgtat cctgccgatg gtggtttaga aggtagaact
      541 gacatggcat tgaaattagt tggtggtggt cacttaattt gcaatttcaa gaccacttac
      601 agatctaaaa agccagctaa aaacttgaag atgcctggtg tatactacgt cgatcataga
      661 ttggaaagaa ttaaagaagc agacaaggaa acatatgttg aacaacacga agtcgctgtt
      721 gcaagatact gcgatttgcc ttccaagtta ggtcataagt tgaacggtat ggacgaattg
      781 tataagtaag gcgcgccatt ataagtaaat gcatgtatac taaactcaca aattagagct
      841 tcaatttaat tatatcagtt attacccggg aatctcggtc gtaatgattt ctataatgac
      901 gaaaaaaaaa aaattggaaa gaaaaagctt catggccttt ataaaaagga actatccaat
      961 acctcgccag aaccaagtaa cagtatttta cggggcacaa atcaagaaca ataagacagg
     1021 actgtaaaga tggacgcata gatccggatg gtataaacgg aaacaaaaaa aagagctggt
     1081 actactttct ttaaaattat tttattattt gattttattt aatagtatat attatatttt
     1141 gaacgtagat tattttgttg aaagttgctg tagtgccatt gattcgtaac actaattctg
     1201 tattagtcat tcctcttgtt tgatagtatc caaaaaaacg gctatttttt tgcaatctta
     1261 tttcctgcat attatacaga taacataatg aaagaaaaaa tctttttttt tgttcttcaa
     1321 tgatgatttc aaccattctt ttaaacattg atcaattcct gagcaacaac cccatacaca
     1381 ctggtttata taccgcccct tttacagttg aagaaagaaa tagaaataga aatagcaaac
     1441 aaaagatatg acagtcaaca ctaagaccta tagtgagaga gcagaaactc atgcctcacc
     1501 agtagcacag cgattatttc gattaatgga actgaagaaa accaatttat gtgcatcaat
     1561 tgacgttgat accactaagg aattccttga attaattgat aaattaggtc cttatgtatg
     1621 cttaatcaag actcatattg atataatcaa tgatttttcc tatgaatcca ctattgaacc
     1681 attattagaa ctttcacgta aacatcaatt tatgattttt gaagatagaa aatttgctga
     1741 tattggtaat accgtaaaga aacaatatat tggtggagtt tataaaatta gtagttgggc
     1801 agatattacc aatgctcatg gtgtcactgg gaatggagtg gttgaaggat taaaacaggg
     1861 agctaaagaa accaccacca accaagagcc aagagggtta ttgatgttag ctgaattatc
     1921 atcagtggga tcattagcat atggagaata ttctcaaaaa actgttgaaa ttgctaaatc
     1981 cgataaggaa tttgttattg gatttattgc ccaacgtgat atgggtggcc aagaagaagg
     2041 atttgattgg cttattatga cacctggagt tggattagat gataaaggtg atggattagg
     2101 acaacaatat agaactgttg atgaagttgt tagcactgga actgatatta tcattgttgg
     2161 tagaggattg tttggtaaag gaagagatcc agatattgaa ggtaaaaggt atagaaatgc
     2221 tggttggaat gcttatttga aaaagactgg ccaattataa atgtgaaggg ggagattttc
     2281 actttattag atttgtatat atgtagaata aataaataaa taagttaaat aaataattaa
     2341 ataagggtgg taattattac tatttacaat caaaggtggt ccttctagtt taaacgagct
     2401 cgaattcatc gatgatatca gatccactag tggcctatgc ggccgcggat ctgccggtct
     2461 ccctatagtg agtcgtatta atttcgataa gccaggttaa cctgcattaa tgaatcggcc
     2521 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     2581 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     2641 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2701 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2761 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2821 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2881 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     2941 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3001 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3061 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3121 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     3181 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3241 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3301 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3361 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     3421 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3481 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3541 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     3601 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3661 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3721 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3781 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3841 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3901 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3961 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4021 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     4081 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     4141 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4201 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4261 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4321 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4381 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4441 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     4501 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     4561 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     4621 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     4681 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     4741 atatggacat attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac
     4801 acatacgatt taggtgacac tata
//
