LOCUS       V006378                 4627 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006378
VERSION     V006378
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Epp E, Nazarova E, Douglas LM, Konopka JB, Vogel J, Whiteway M.
  TITLE     Actin dynamics and endocytosis in the absence of Arp2/3 in Candida
            albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Epp E, Nazarova E, Douglas LM, Konopka JB, Vogel J, Whiteway M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2011) Biology, McGill University, 1205 Docteur
            Penfield, Montreal, Quebec H3A 1B1, Canada
REFERENCE   3  (bases 1 to 4627)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4627)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-MAY-2011) Biology, McGill University, 1205 Docteur Penfield,
            Montreal, Quebec H3A 1B1, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             58..786
                     /label="TagRFP-T"
                     /note="photostable variant of TagRFP (Shaner et al., 2008)"
     regulatory      792..1045
                     /label="Saccharomyces cerevisiae URA3 terminator"
                     /note="Saccharomyces cerevisiae URA3 terminator"
                     /regulatory_class="terminator"
     CDS             1187..2080
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     promoter        complement(2265..2283)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2541..3129)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3303..4160)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4161..4265)
                     /label="AmpR promoter"
     promoter        4611..4627
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcagggtg ctggcgcagg tgcttcggta
       61 tctaagggtg aagaattgat taaggaaaac atgcacatga agttgtatat ggaaggtact
      121 gtaaataacc accattttaa gtgtacctct gaaggtgaag gtaaaccata tgaaggtaca
      181 caaaccatga gaattaaagt tgtagaaggt ggtccattgc cttttgcctt cgatatctta
      241 gctacatctt ttatgtacgg ttcaagaacc ttcatcaatc atactcaagg tataccagat
      301 ttctttaagc aatcattccc tgaaggtttc acttgggaaa gagtcactac atatgaagac
      361 ggtggtgttt tgactgccac acaagataca tccttgcaag acggttgttt aatctataac
      421 gttaagatca gaggtgtaaa cttcccaagt aacggtcctg taatgcaaaa gaaaactttg
      481 ggttgggaag caaacaccga aatgttgtat cctgccgatg gtggtttaga aggtagaact
      541 gacatggcat tgaaattagt tggtggtggt cacttaattt gcaatttcaa gaccacttac
      601 agatctaaaa agccagctaa aaacttgaag atgcctggtg tatactacgt cgatcataga
      661 ttggaaagaa ttaaagaagc agacaaggaa acatatgttg aacaacacga agtcgctgtt
      721 gcaagatact gcgatttgcc ttccaagtta ggtcataagt tgaacggtat ggacgaattg
      781 tataagtaag gcgcgccatt ataagtaaat gcatgtatac taaactcaca aattagagct
      841 tcaatttaat tatatcagtt attacccggg aatctcggtc gtaatgattt ctataatgac
      901 gaaaaaaaaa aaattggaaa gaaaaagctt catggccttt ataaaaagga actatccaat
      961 acctcgccag aaccaagtaa cagtatttta cggggcacaa atcaagaaca ataagacagg
     1021 actgtaaaga tggacgcata gatccaacgt gtctcaaagc gataacgggt acaaaaaaaa
     1081 atgtattgag tcattgaaat ttttttattt tttttttttt ttggtgaaga tttttcccac
     1141 accaaatata taccactttt cttctaacac cacctacata ttcactatgg atttagtcaa
     1201 tcatttgccc gatcgtttgt tatttgctgt tcccaaaaag ggcagattat atgaaaaatg
     1261 ctgcagctta ttgagcggtg ccgatataca gtttagaaga tctaatagat tggatattgc
     1321 cctttccaca aacttgccaa ttgcattaat ctttttgcct gcagccgata ttccagtttt
     1381 cgttggagaa ggtaattgtg acttgggtat aactgggtta gaccagatca aagaagcaga
     1441 ccaattcgac aacattgagg atttgttgga tttgaagttt ggctcatgta aattgcagat
     1501 ccaagttcct gcaaatggcg agtacgaaaa acccgaacaa ctcgttggaa agaagattgt
     1561 atcctccttt acaaagttga gtactgacta tttcaagaaa ttatccgaca aacctacgaa
     1621 tattagatac gttggtggtt cagttgaggc ttcttgcgca ttgggtgttg ccgacgccat
     1681 tgtcgattta gttgaaagtg gagagaccat gaaagctgct ggattaaagg cgatcgaaac
     1741 catattggaa acttccgctc atttgatttc ctccaagaaa accaaatttc cagaaatggt
     1801 caacataatt gtccaaagac ttcaaggtgt tttagcggct caggaatatg tcttgtgtaa
     1861 ctacaatgct ccaaaatcca ttcaatcaaa gtgcttaacc attactccag gtagaagggc
     1921 agccactgtc tccactttgg ataaacacag tgacgacgaa gatgactggg ttgccatttc
     1981 ttccatggtt aacagaaagg aaattggtaa tgtaatggat gaattgaaga aagctggcgc
     2041 aacagatata ttggtgctcg agatatcaaa ttgtagagtt taataaaata ctatatatac
     2101 atacttatag atttacttca atttgactca atctgtctta taccatcttt atctaccacc
     2161 ttgatgtaca cacctttaaa atcaatgggc gtttaaacga gctcgaattc atcgatgata
     2221 tcagatccac tagtggccta tgcggccgcg gatctgccgg tctccctata gtgagtcgta
     2281 ttaatttcga taagccaggt taacctgcat taatgaatcg gccaacgcgc ggggagaggc
     2341 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2401 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2461 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2521 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2581 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2641 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2701 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     2761 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2821 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2881 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2941 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     3001 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3061 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3121 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3181 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3241 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3301 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3361 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3421 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3481 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3541 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3601 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     3661 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     3721 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     3781 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3841 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3901 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3961 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4021 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4081 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4141 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4201 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4261 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     4321 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     4381 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     4441 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     4501 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgga catattgtcg
     4561 ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga
     4621 cactata
//
