LOCUS       40924_19131        5433 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFA-TagRFP-T-ARG4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Epp E, Nazarova E, Douglas LM, Konopka JB, Vogel J, Whiteway M.
  TITLE     Actin dynamics and endocytosis in the absence of Arp2/3 in Candida 
            albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   Epp E, Nazarova E, Douglas LM, Konopka JB, Vogel J, Whiteway M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2011) Biology, McGill University, 1205 Docteur 
            Penfield, Montreal, Quebec H3A 1B1, Canada
REFERENCE   3  (bases 1 to 5433)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5433)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAY-2011) Biology, McGill University, 1205 Docteur Penfield, 
            Montreal, Quebec H3A 1B1, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             58..786
                     /label=TagRFP-T
                     /note="photostable variant of TagRFP (Shaner et al., 2008)"
     regulatory      792..1045
                     /label=Saccharomyces cerevisiae URA3 terminator
                     /note="Saccharomyces cerevisiae URA3 terminator"
                     /regulatory_class="terminator"
     CDS             1503..2909
                     /codon_start=1
                     /gene="ARG4"
                     /product="Arg4"
                     /label=ARG4
                     /protein_id="AEI30135.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELNLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF
                     DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG
                     ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDALGGTAKSAVLK
                     QLENLKSILS"
     gene            1503..2909
                     /gene="ARG4"
                     /label=ARG4
                     /note="selectable marker ARG4 from C. albicans"
     promoter        complement(3071..3089)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3347..3935)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4109..4966)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4967..5071)
                     /label=AmpR promoter
     promoter        5417..5433
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcagggtg ctggcgcagg tgcttcggta
       61 tctaagggtg aagaattgat taaggaaaac atgcacatga agttgtatat ggaaggtact
      121 gtaaataacc accattttaa gtgtacctct gaaggtgaag gtaaaccata tgaaggtaca
      181 caaaccatga gaattaaagt tgtagaaggt ggtccattgc cttttgcctt cgatatctta
      241 gctacatctt ttatgtacgg ttcaagaacc ttcatcaatc atactcaagg tataccagat
      301 ttctttaagc aatcattccc tgaaggtttc acttgggaaa gagtcactac atatgaagac
      361 ggtggtgttt tgactgccac acaagataca tccttgcaag acggttgttt aatctataac
      421 gttaagatca gaggtgtaaa cttcccaagt aacggtcctg taatgcaaaa gaaaactttg
      481 ggttgggaag caaacaccga aatgttgtat cctgccgatg gtggtttaga aggtagaact
      541 gacatggcat tgaaattagt tggtggtggt cacttaattt gcaatttcaa gaccacttac
      601 agatctaaaa agccagctaa aaacttgaag atgcctggtg tatactacgt cgatcataga
      661 ttggaaagaa ttaaagaagc agacaaggaa acatatgttg aacaacacga agtcgctgtt
      721 gcaagatact gcgatttgcc ttccaagtta ggtcataagt tgaacggtat ggacgaattg
      781 tataagtaag gcgcgccatt ataagtaaat gcatgtatac taaactcaca aattagagct
      841 tcaatttaat tatatcagtt attacccggg aatctcggtc gtaatgattt ctataatgac
      901 gaaaaaaaaa aaattggaaa gaaaaagctt catggccttt ataaaaagga actatccaat
      961 acctcgccag aaccaagtaa cagtatttta cggggcacaa atcaagaaca ataagacagg
     1021 actgtaaaga tggacgcata gatcccccct ttagtaagat ttttcaagag tagtctcaaa
     1081 taaacagaat gaaggattga aggttttatt caataaataa ttggacagaa agttattgca
     1141 cagttttaac aacaacaatt tcaaaatttc aatttatgac atattgaccg acataattca
     1201 gtcatttcta atattcaaat aacaagacac cttgtataaa tgaatccaca agcgtatatg
     1261 aacaaccaaa ttttatgact aactgtgggt cgtgtacttt tgacgattga gtcattttta
     1321 ccaattaggt aattgaattt tactaatttg tcattaaccg cgctggccat cccaacaaca
     1381 acaacaacaa tcaatttaaa aacttgacaa ggcaaaaaaa aaaaatttca tctttgaaaa
     1441 atttttcttc cttttcttct tctgttcttt acataacagc tatcaagaat aatcaattaa
     1501 taatgtcaca acaacaagat aaacaaccta gtgaaaataa attatggggt ggtcgtttca
     1561 ccggtgccac tgatccattg atggatttgt ataatgcttc attaccttat gataaagtta
     1621 tgtatgatgc tgatttaact ggtactaaag tatacacaca aggattaaac aaattaggat
     1681 taattaccac tgaagaattg aatttgattc atcaaggatt agaacaaatc agacaagaat
     1741 ggcatgataa taaattcatt attaaggctg gtgatgagga tattcatact gctaatgaac
     1801 gtcgattagg agaaatcatt gggaaaaaca tttctggtaa agttcacact ggtagatcaa
     1861 gaaatgatca agttgctacc gatatgagaa ttttcgttcg tgaatcatta cttaatttaa
     1921 gtaaaatttt acatcaattc atcacggcaa ttcttgaacg agcacataaa gaaatcgatg
     1981 tattaatgcc tggttatact catttacaaa aagctcaacc tattagatgg gcccattggt
     2041 taagttcata tgcaacttat ttcaccgaag attataaacg tttacaagaa attataactc
     2101 gtgttaacca atctccatta ggatctggtg ctttagcagg tcatccttat ggtattgatc
     2161 gtgaattttt agctaaagga ttaggatttg atggtgtcat tgggaattct ttaactgcag
     2221 tttcagatcg tgattttgtt gttgaaagtt tattttggag tacattattt atgaatcata
     2281 tttctcgatt ctcagaagat ctaattattt attctagtgg tgaatttggg ttcataaaat
     2341 tagctgatgc ttattctact ggttcttcat tgatgccaca aaagaaaaac cctgactcat
     2401 tagaattatt acgtgggaaa agtggtagag tatttggtca gttatctgga tttttaatgt
     2461 cgatcaaatc tattccatct acttataata aagatatgca agaggataaa gaaccattat
     2521 ttgatgcatt gactacagta gaacattcaa ttttaattgc tactggtgtg atttctactt
     2581 tactgattga taaacaaaat atggaaaaag ctttaactat ggatatgttg gctactgatt
     2641 tagctgatta tttagttaga aaaggagttc catttagaga aactcatcat atatctggtg
     2701 aatgtgttag aaaagctgaa gaagaaaaat tatctggaat tgatcaatta tcttttgaac
     2761 aatttcaaca aattgattct cgttttgaaa aagacgttat ggaaactttt gattttgaag
     2821 ctagtgtgga aagaagagat gctcttggtg gtactgctaa aagtgccgtt ttaaaacaat
     2881 tagaaaattt aaaatcaatc ttaagttaat ttataaatag tcatataata atcacagtat
     2941 tgtttaactt acataaaata taatcaatta acacagagat accttgtact acaatagttt
     3001 aaacgagctc gaattcatcg atgatatcag atccactagt ggcctatgcg gccgcggatc
     3061 tgccggtctc cctatagtga gtcgtattaa tttcgataag ccaggttaac ctgcattaat
     3121 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     3181 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     3241 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3301 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3361 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3421 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3481 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3541 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3601 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3661 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3721 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3781 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3841 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3901 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3961 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     4021 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     4081 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     4141 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     4201 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     4261 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     4321 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4381 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     4441 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4501 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     4561 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4621 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4681 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4741 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4801 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4861 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4921 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4981 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     5041 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     5101 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     5161 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     5221 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     5281 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     5341 gagtgcacca tatggacata ttgtcgttag aacgcggcta caattaatac ataaccttat
     5401 gtatcataca catacgattt aggtgacact ata
//
