LOCUS       V016002                 4805 bp    DNA     circular SYN 17-JUL-2019
DEFINITION  Exported.
ACCESSION   V016002
VERSION     V016002
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     A new toolkit for gene tagging in Candida albicans containing
            recyclable markers
  JOURNAL   PLoS ONE (2019) In press
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Instituto de Biologia Funcional y Genomica
            (IBFG), Consejo Superior de Investigaciones Cientificas, Zacarias
            Gonzalez 2, Salamanca, Salamanca 37007, Spain
REFERENCE   3  (bases 1 to 4805)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 13
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE
            (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAR-2019) Instituto de Biologia Funcional y Genomica (IBFG),
            Consejo Superior de Investigaciones Cientificas, Zacarias Gonzalez
            2, Salamanca, Salamanca 37007, Spain"
FEATURES             Location/Qualifiers
     source          1..4805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             69..146
                     /label="CBP"
                     /note="calmodulin-binding peptide"
     CDS             174..194
                     /label="TEV site"
                     /note="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
     CDS             225..398
                     /label="ProtA"
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
     regulatory      594..849
                     /note="Transcriptional termination from Saccharomyces
                     cerevisiae URA3 gene"
                     /regulatory_class="terminator"
     primer_bind     859..875
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    889..922
                     /label="loxP"
                     /note="loxP"
     CDS             1352..2161
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     protein_bind    complement(2312..2345)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(2443..2461)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2719..3307)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3481..4338)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4339..4443)
                     /label="AmpR promoter"
     promoter        4789..4805
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 atccatggaa gagaagatgg aaaaagaatt tcatagccgt ctcagcagcc aaccgcttta
      121 agaaaatctc atcctccggg gcacttgatt atgatattcc aactactgct agcgagaatt
      181 tgtattttca gggagaattc ggccttgcgc aacacgatga agccgtggac aacaaattca
      241 acaaagaaca acaaaacgcg ttctatgaga tcttacattt acctaactta aacgaagaac
      301 aacgaaacgc cttcatccaa agtttaaaag atgacccaag ccaaagcgct aaccttttag
      361 cagaagctaa aaagctaaat gatgctcagg cgccgaaagt agacaacaaa ttcaacaaag
      421 aacaacaaaa cgcgttctat gagatcttac atttacctaa cttaaacgaa gaacaacgaa
      481 acgccttcat ccaaagttta aaagatgacc caagccaaag cgctaacctt ttagcagaag
      541 ctaaaaagct aaatgatgct caggcgccga aagtagacgc gaatcatcag tgaggcgcgc
      601 cattataagt aaatgcatgt atactaaact cacaaattag agcttcaatt taattatatc
      661 agttattacc cgggaatctc ggtcgtaatg atttctataa tgacgaaaaa aaaaaaattg
      721 gaaagaaaaa gcttcatggc ctttataaaa aggaactatc caatacctcg ccagaaccaa
      781 gtaacagtat tttacggggc acaaatcaag aacaataaga caggactgta aagatggacg
      841 catagatccg gcggccgctc tagaactagt ggatccagat cttgatccat aacttcgtat
      901 agcatacatt atacgaagtt atagatccag tactaatagg aattgatttg gatggtataa
      961 acggaaacaa aaaaaagagc tggtactact ttctttaaaa ttattttatt atttgatttt
     1021 atttaatagt atatattata ttttgaacgt agattatttt gttgaaagtt gctgtagtgc
     1081 cattgattcg taacactaat tctgtattag tcattcctct tgtttgatag tatccaaaaa
     1141 aacggctatt tttttgcaat cttatttcct gcatattata cagataacat aatgaaagaa
     1201 aaaatctttt tttttgttct tcaatgatga tttcaaccat tcttttaaac attgatcaat
     1261 tcctgagcaa caaccccata cacactggtt tatatatcgc cccttttaca gttgaagaaa
     1321 gaaatagaaa tagaaatagc aaacaaaaga tatgacagtc aacactaaga cctatagtga
     1381 gagagcagaa actcatgcct caccagtagc acaacgatta tttcgattaa tggaactgaa
     1441 gaaaaccaat ttatgtgcat caattgatgt tgataccact aaggaattcc ttgaattaat
     1501 tgataaattg ggtccttatg tatgcttaat caagactcat attgatataa tcaatgattt
     1561 ttcctatgaa tccactattg aaccattatt agaactttca cgtaaacatc aatttatgat
     1621 ttttgaagat agaaaatttg ctgatattgg taataccgtg aagaaacaat atattggtgg
     1681 agtttataaa attagtagtt gggcagatat tactaatgct catggtgtca ctgggaatgg
     1741 agtagttgaa ggattaaaac agggagctaa agaaaccacc accaaccaag agccaagagg
     1801 gttattgatg ttagctgaat tatcatcagt gggatcatta gcatatggag aatattctca
     1861 aaaaactgtt gaaattgcta aatccgataa ggaatttgtt attggattta ttgcccaacg
     1921 tgatatgggt ggacaagaag aaggatttga ttggcttatt atgacacctg gagttggatt
     1981 agatgataaa ggtgatggat taggacaaca atatagaact gttgatgaag ttgttagcac
     2041 tggaactgat attatcattg ttggtagagg attgtttggt aaaggaagag atccagatat
     2101 tgaaggtaaa aggtatagag atgctggttg gaatgcttat ttgaaaaaaa ctggccaatt
     2161 ataaatgtga agggggagat tttcacttta ttagatttgt atatatgtat aataaataaa
     2221 taaataagtt aaataaataa ttaaataagg gtggtaatta ttactattta caatcaaagg
     2281 tggtccttct agaagatctg ctagcccatg gataacttcg tatagcatac attatacgaa
     2341 gttattaggg ataacagggt aatctgcagg aaaaacgagc tcgaattcat cgatgatatc
     2401 agatccacta gtggcctatg cggccgcgga tctgccggtc tccctatagt gagtcgtatt
     2461 aatttcgata agccaggtta acctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     2521 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     2581 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2641 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2701 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2761 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2821 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2881 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     2941 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3001 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3061 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3121 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     3181 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3241 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3301 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3361 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3421 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3481 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3541 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3601 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3661 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3721 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3781 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3841 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3901 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3961 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4021 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4081 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4141 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     4201 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4261 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4321 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4381 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4441 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     4501 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     4561 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     4621 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     4681 taactatgcg gcatcagagc agattgtact gagagtgcac catatggaca tattgtcgtt
     4741 agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat ttaggtgaca
     4801 ctata
//
