LOCUS       V016530                 4699 bp    DNA     circular SYN 17-JUL-2019
DEFINITION  Exported.
ACCESSION   V016530
VERSION     V016530
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     A new toolkit for gene tagging in Candida albicans containing
            recyclable markers
  JOURNAL   PLoS ONE (2019) In press
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Instituto de Biologia Funcional y Genomica
            (IBFG), Consejo Superior de Investigaciones Cientificas, Zacarias
            Gonzalez 2, Salamanca, Salamanca 37007, Spain
REFERENCE   3  (bases 1 to 4699)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 13
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE
            (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAR-2019) Instituto de Biologia Funcional y Genomica (IBFG),
            Consejo Superior de Investigaciones Cientificas, Zacarias Gonzalez
            2, Salamanca, Salamanca 37007, Spain"
FEATURES             Location/Qualifiers
     source          1..4699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             69..146
                     /label="CBP"
                     /note="calmodulin-binding peptide"
     CDS             174..194
                     /label="TEV site"
                     /note="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
     CDS             225..398
                     /label="ProtA"
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
     regulatory      594..849
                     /note="Transcriptional termination from Saccharomyces
                     cerevisiae URA3 gene"
                     /regulatory_class="terminator"
     primer_bind     859..875
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    889..922
                     /label="loxP"
                     /note="loxP"
     CDS             1270..2163
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     protein_bind    complement(2206..2239)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(2337..2355)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2613..3201)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3375..4232)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4233..4337)
                     /label="AmpR promoter"
     promoter        4683..4699
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 atccatggaa gagaagatgg aaaaagaatt tcatagccgt ctcagcagcc aaccgcttta
      121 agaaaatctc atcctccggg gcacttgatt atgatattcc aactactgct agcgagaatt
      181 tgtattttca gggagaattc ggccttgcgc aacacgatga agccgtggac aacaaattca
      241 acaaagaaca acaaaacgcg ttctatgaga tcttacattt acctaactta aacgaagaac
      301 aacgaaacgc cttcatccaa agtttaaaag atgacccaag ccaaagcgct aaccttttag
      361 cagaagctaa aaagctaaat gatgctcagg cgccgaaagt agacaacaaa ttcaacaaag
      421 aacaacaaaa cgcgttctat gagatcttac atttacctaa cttaaacgaa gaacaacgaa
      481 acgccttcat ccaaagttta aaagatgacc caagccaaag cgctaacctt ttagcagaag
      541 ctaaaaagct aaatgatgct caggcgccga aagtagacgc gaatcatcag tgaggcgcgc
      601 cattataagt aaatgcatgt atactaaact cacaaattag agcttcaatt taattatatc
      661 agttattacc cgggaatctc ggtcgtaatg atttctataa tgacgaaaaa aaaaaaattg
      721 gaaagaaaaa gcttcatggc ctttataaaa aggaactatc caatacctcg ccagaaccaa
      781 gtaacagtat tttacggggc acaaatcaag aacaataaga caggactgta aagatggacg
      841 catagatccg gcggccgctc tagaactagt ggatccccat ggtgatccat aacttcgtat
      901 agcatacatt atacgaagtt atagatccgg tacctggagg atgaggagac agaagttagt
      961 agtaacaatt tggggacaga agaggataca gtgacatcag aggaagaaga cagcgaaggt
     1021 atagtcaaaa agcaatagga cagcaagaca atttatttat ttaatagttt tttataaatg
     1081 taaaatctat cacaacatgt gtacttaatt ttctgtggta ggggaggcca tttcgttgaa
     1141 acgtgtcgcg agcggttgtg agaattgact cattttgatt attacgcaac cattgaaatt
     1201 ttttattttt ttttggtgaa gatttttccc acacaacttc ttcttttact taaccaccaa
     1261 ctaccgataa tggatttagt caatcattta ccagaccgtt tgttatttgc tgttcctaaa
     1321 aagggcagat tatacgaaaa atgctgtaac ttattgagtg gtgccgatat acagtttaga
     1381 agatctaata gattagatat agcactttct acaaacttgc caattgcatt aatcttcttg
     1441 cctgcagctg atatcccagt tttcgttgga gaaggtaatt gtgacttggg tataactggg
     1501 ttagaccaaa tcaaagaagc tgaacaattc gacaacatcg aggacttgtt ggatttgaaa
     1561 tttggttcat gcaaattgca gatccaagtt ccagcagatg gcgagtacga aaagccagaa
     1621 cagcttgttg gaaagaaaat tgtgtcttca tttacaaaat tgagtaccga ctatttcaaa
     1681 caattgtcag acaaacctac taatatcaga tatgtcggtg gttccgttga ggcttcttgt
     1741 gccttgggtg ttgctgatgc tattgtcgat ttggttgaaa gtggtgaaac tatgaaagca
     1801 gctgggttaa aggccataga aaccatattg gagacttcgg ctcatttgat ttcgtcaaag
     1861 aaaagtaaat tcccagaaat ggtcaatata atcgtccaaa gacttcaagg tgttttagct
     1921 gcccaggaat atgtcttgtg taactacaat gccccaaaat ccatccaagc aaaatgttta
     1981 accattactc caggcagaag ggccgctact gtctctactt tggacaaaca cagcgacgac
     2041 gaagaggact gggttgccat ctcatccatg gttaatagaa aggaaatcgg taatgtaatg
     2101 gacgaattga agaaagctgg tgcaaccgat atattggtac tcgagatatc aaattgtaga
     2161 gtttaaaaga agtgatagtt tctcataaat attgctagcc catggataac ttcgtatagc
     2221 atacattata cgaagttatt agggataaca gggtaatctg caggaaaaac gagctcgaat
     2281 tcatcgatga tatcagatcc actagtggcc tatgcggccg cggatctgcc ggtctcccta
     2341 tagtgagtcg tattaatttc gataagccag gttaacctgc attaatgaat cggccaacgc
     2401 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     2461 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2521 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2581 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2641 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2701 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2761 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
     2821 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2881 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2941 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     3001 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     3061 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     3121 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3181 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3241 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3301 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3361 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3421 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3481 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3541 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3601 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3661 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3721 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3781 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3841 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3901 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3961 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     4021 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     4081 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     4141 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     4201 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     4261 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     4321 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     4381 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     4441 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     4501 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     4561 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     4621 gacatattgt cgttagaacg cggctacaat taatacataa ccttatgtat catacacata
     4681 cgatttaggt gacactata
//
