LOCUS       62056_10660        5405 bp DNA     circular SYN 17-JUL-2019
DEFINITION  Cloning vector pFA-TAP-LAL, complete sequence.
ACCESSION   MK652130
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F, 
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     A new toolkit for gene tagging in Candida albicans containing 
            recyclable markers
  JOURNAL   PLoS ONE (2019) In press
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F, 
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Instituto de Biologia Funcional y Genomica 
            (IBFG), Consejo Superior de Investigaciones Cientificas, Zacarias 
            Gonzalez 2, Salamanca, Salamanca 37007, Spain
REFERENCE   3  (bases 1 to 5405)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE 
            (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-MAR-2019) Instituto de Biologia Funcional y Genomica (IBFG), 
            Consejo Superior de Investigaciones Cientificas, Zacarias Gonzalez 
            2, Salamanca, Salamanca 37007, Spain"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 13
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             69..146
                     /label=CBP
                     /note="calmodulin-binding peptide"
     CDS             174..194
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             225..398
                     /label=ProtA
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
     regulatory      594..849
                     /note="Transcriptional termination from Saccharomyces
                     cerevisiae URA3 gene"
                     /regulatory_class="terminator"
     primer_bind     859..875
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    877..910
                     /label=loxP
                     /note="loxP"
     gene            911..2911
                     /gene="ARG4"
                     /label=ARG4
     CDS             1377..2783
                     /codon_start=1
                     /transl_table=11
                     /gene="ARG4"
                     /product="ARG4"
                     /label=ARG4
                     /note="argininosuccinate lyase; derived from Candida
                     albicans ARG4"
                     /protein_id="QDK59790.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELHLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF
                     DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG
                     ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDAIGGTAKSAVLK
                     QLENLKSILS"
     protein_bind    complement(2912..2945)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(3043..3061)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3319..3907)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4081..4938)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4939..5043)
                     /label=AmpR promoter
     promoter        5389..5405
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 atccatggaa gagaagatgg aaaaagaatt tcatagccgt ctcagcagcc aaccgcttta
      121 agaaaatctc atcctccggg gcacttgatt atgatattcc aactactgct agcgagaatt
      181 tgtattttca gggagaattc ggccttgcgc aacacgatga agccgtggac aacaaattca
      241 acaaagaaca acaaaacgcg ttctatgaga tcttacattt acctaactta aacgaagaac
      301 aacgaaacgc cttcatccaa agtttaaaag atgacccaag ccaaagcgct aaccttttag
      361 cagaagctaa aaagctaaat gatgctcagg cgccgaaagt agacaacaaa ttcaacaaag
      421 aacaacaaaa cgcgttctat gagatcttac atttacctaa cttaaacgaa gaacaacgaa
      481 acgccttcat ccaaagttta aaagatgacc caagccaaag cgctaacctt ttagcagaag
      541 ctaaaaagct aaatgatgct caggcgccga aagtagacgc gaatcatcag tgaggcgcgc
      601 cattataagt aaatgcatgt atactaaact cacaaattag agcttcaatt taattatatc
      661 agttattacc cgggaatctc ggtcgtaatg atttctataa tgacgaaaaa aaaaaaattg
      721 gaaagaaaaa gcttcatggc ctttataaaa aggaactatc caatacctcg ccagaaccaa
      781 gtaacagtat tttacggggc acaaatcaag aacaataaga caggactgta aagatggacg
      841 catagatccg gcggccgctc tagaactagt ggatccataa cttcgtatag catacattat
      901 acgaagttat agatccgtac ccccctttag taagattttt caagagtagt ctcaaataaa
      961 cagaatgaag gattgaaggt tttattcaat aaataattgg acagaaagtt attgtacagt
     1021 tttaacaaca acaatttcaa aatttcaatt tatgacatat tgaccgacat aattcagtca
     1081 tttctaatat tcaaataaca agacaccttg tataaatgaa tccacaatcg tatatgaaca
     1141 accaaatttt atgactaact ttgggtcgtg tacttttgac gattgagtca tttttaccaa
     1201 ttaggtaatt gaattttgtt aatttgtcat taaccgcgct ggccatccca acaataacaa
     1261 caatcaattt aaaaacttga caaggcaaaa aaaaaaaaat tttatctttg aaaaattttt
     1321 cttccttttc ttcttctgtt ctttacataa cagctatcaa gaataatcaa ttaataatgt
     1381 cacaacaaca agataaacaa cctagtgaaa ataaattatg gggtggtcgt ttcaccggtg
     1441 ccactgatcc attgatggat ttatataatg cttcattacc ttatgataaa gttatgtatg
     1501 atgctgattt aactggtact aaagtataca cacaaggatt aaacaaatta ggattaatta
     1561 ccactgaaga attgcatttg attcatcaag gattagaaca aatcagacaa gaatggcatg
     1621 ataataaatt cattattaag gcaggtgatg aggatattca tactgctaat gaacgtcgat
     1681 taggagaaat cattgggaaa aacatttctg gtaaagttca cactggtaga tcaagaaatg
     1741 atcaagttgc taccgatatg agaattttcg ttcgtgaatc attacttaat ttaagtaaaa
     1801 ttttacatca attcatcacg gcaattcttg aacgagcaca taaagaaatc gatgtattaa
     1861 tgcctggtta tactcattta caaaaagctc aacctattag atgggcccat tggttaagtt
     1921 catatgcaac ttatttcacc gaagattata aacgtttaca agaaattata actcgtgtta
     1981 accaatctcc attaggatct ggtgctttag caggtcatcc ttatggtatt gatcgtgaat
     2041 ttttagctaa aggattagga tttgatggtg tcattgggaa ttctttaact gcagtttcag
     2101 atcgtgattt tgttgttgaa agtttatttt ggagtacatt atttatgaat catatttctc
     2161 gattctcaga agatctaatt atttattcta gtggtgaatt tgggttcata aaattagctg
     2221 atgcttattc tactggttct tcattgatgc cacaaaagaa aaaccctgac tcattagaat
     2281 tattacgtgg gaaaagtggt agagtatttg gtcagttatc tggattttta atgtcgatca
     2341 aatctattcc atctacttat aataaagata tgcaagagga taaagaacca ttatttgatg
     2401 cattgactac agtggaacat tcaattttaa ttgctactgg tgtgatttct actttactga
     2461 ttgataaaca aaatatggaa aaagctttaa ctatggatat gttggctact gatttagctg
     2521 attatttagt tagaaaagga gttccattta gagaaactca tcatatatct ggtgaatgtg
     2581 ttagaaaagc tgaagaagaa aaattatctg gaattgatca attatctttt gaacaatttc
     2641 aacaaattga ttctcgtttt gaaaaagacg ttatggaaac ttttgatttt gaagctagtg
     2701 tggaaagaag agatgctatt ggtggtactg ctaaaagtgc cgttttaaaa caattagaaa
     2761 atttaaaatc aattttaagt taatttataa atagtcatat aataatcaca gtattgttta
     2821 acttacataa aatataatca attaacacag agataccttg tactccaata ctagcattcc
     2881 aaccagcatt tctatacctg ctagcccatg gataacttcg tatagcatac attatacgaa
     2941 gttattaggg ataacagggt aatctgcagg aaaaacgagc tcgaattcat cgatgatatc
     3001 agatccacta gtggcctatg cggccgcgga tctgccggtc tccctatagt gagtcgtatt
     3061 aatttcgata agccaggtta acctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3121 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3181 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3241 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3301 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3361 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3421 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3481 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     3541 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3601 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3661 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3721 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     3781 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3841 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3901 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3961 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4021 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4081 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4141 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4201 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     4261 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     4321 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     4381 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     4441 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     4501 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     4561 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4621 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4681 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4741 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     4801 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4861 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4921 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4981 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5041 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     5101 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     5161 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     5221 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     5281 taactatgcg gcatcagagc agattgtact gagagtgcac catatggaca tattgtcgtt
     5341 agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat ttaggtgaca
     5401 ctata
//
