LOCUS       V015289                 3999 bp    DNA     circular SYN 17-JUL-2019
DEFINITION  Exported.
ACCESSION   V015289
VERSION     V015289
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     A new toolkit for gene tagging in Candida albicans containing
            recyclable markers
  JOURNAL   PLoS ONE (2019) In press
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Instituto de Biologia Funcional y Genomica
            (IBFG), Consejo Superior de Investigaciones Cientificas, Zacarias
            Gonzalez 2, Salamanca, Salamanca 37007, Spain
REFERENCE   3  (bases 1 to 3999)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 13
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE
            (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAR-2019) Instituto de Biologia Funcional y Genomica (IBFG),
            Consejo Superior de Investigaciones Cientificas, Zacarias Gonzalez
            2, Salamanca, Salamanca 37007, Spain"
FEATURES             Location/Qualifiers
     source          1..3999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     53..69
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    83..116
                     /label="loxP"
                     /note="loxP"
     CDS             546..1355
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     protein_bind    complement(1506..1539)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(1637..1655)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1913..2501)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2675..3532)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3533..3637)
                     /label="AmpR promoter"
     promoter        3983..3999
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acgggcggcc gctctagaac
       61 tagtggatcc agatcttgat ccataacttc gtatagcata cattatacga agttatagat
      121 ccagtactaa taggaattga tttggatggt ataaacggaa acaaaaaaaa gagctggtac
      181 tactttcttt aaaattattt tattatttga ttttatttaa tagtatatat tatattttga
      241 acgtagatta ttttgttgaa agttgctgta gtgccattga ttcgtaacac taattctgta
      301 ttagtcattc ctcttgtttg atagtatcca aaaaaacggc tatttttttg caatcttatt
      361 tcctgcatat tatacagata acataatgaa agaaaaaatc tttttttttg ttcttcaatg
      421 atgatttcaa ccattctttt aaacattgat caattcctga gcaacaaccc catacacact
      481 ggtttatata tcgccccttt tacagttgaa gaaagaaata gaaatagaaa tagcaaacaa
      541 aagatatgac agtcaacact aagacctata gtgagagagc agaaactcat gcctcaccag
      601 tagcacaacg attatttcga ttaatggaac tgaagaaaac caatttatgt gcatcaattg
      661 atgttgatac cactaaggaa ttccttgaat taattgataa attgggtcct tatgtatgct
      721 taatcaagac tcatattgat ataatcaatg atttttccta tgaatccact attgaaccat
      781 tattagaact ttcacgtaaa catcaattta tgatttttga agatagaaaa tttgctgata
      841 ttggtaatac cgtgaagaaa caatatattg gtggagttta taaaattagt agttgggcag
      901 atattactaa tgctcatggt gtcactggga atggagtagt tgaaggatta aaacagggag
      961 ctaaagaaac caccaccaac caagagccaa gagggttatt gatgttagct gaattatcat
     1021 cagtgggatc attagcatat ggagaatatt ctcaaaaaac tgttgaaatt gctaaatccg
     1081 ataaggaatt tgttattgga tttattgccc aacgtgatat gggtggacaa gaagaaggat
     1141 ttgattggct tattatgaca cctggagttg gattagatga taaaggtgat ggattaggac
     1201 aacaatatag aactgttgat gaagttgtta gcactggaac tgatattatc attgttggta
     1261 gaggattgtt tggtaaagga agagatccag atattgaagg taaaaggtat agagatgctg
     1321 gttggaatgc ttatttgaaa aaaactggcc aattataaat gtgaaggggg agattttcac
     1381 tttattagat ttgtatatat gtataataaa taaataaata agttaaataa ataattaaat
     1441 aagggtggta attattacta tttacaatca aaggtggtcc ttctagaaga tctgctagcc
     1501 catggataac ttcgtatagc atacattata cgaagttatt agggataaca gggtaatctg
     1561 caggaaaaac gagctcgaat tcatcgatga tatcagatcc actagtggcc tatgcggccg
     1621 cggatctgcc ggtctcccta tagtgagtcg tattaatttc gataagccag gttaacctgc
     1681 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     1741 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1801 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1861 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1921 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1981 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2041 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2101 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2161 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2221 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2281 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2341 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2401 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2461 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2521 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2581 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2641 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2701 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2761 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2821 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2881 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2941 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3001 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3061 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3121 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3181 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3241 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3301 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3361 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3421 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3481 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3541 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3601 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     3661 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     3721 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     3781 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     3841 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     3901 tactgagagt gcaccatatg gacatattgt cgttagaacg cggctacaat taatacataa
     3961 ccttatgtat catacacata cgatttaggt gacactata
//
