LOCUS       V006382                 5074 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006382
VERSION     V006382
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Epp E, Nazarova E, Douglas LM, Konopka JB, Vogel J, Whiteway M.
  TITLE     Actin dynamics and endocytosis in the absence of Arp2/3 in Candida
            albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Epp E, Nazarova E, Douglas LM, Konopka JB, Vogel J, Whiteway M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2011) Biology, McGill University, 1205 Docteur
            Penfield, Montreal, Quebec H3A 1B1, Canada
REFERENCE   3  (bases 1 to 5074)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5074)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-MAY-2011) Biology, McGill University, 1205 Docteur Penfield,
            Montreal, Quebec H3A 1B1, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             151..201
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et
                     al., 2008)"
                     /label="Lifeact"
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             226..936
                     /label="GFP"
                     /note="green fluorescent protein"
     regulatory      940..1195
                     /label="Saccharomyces cerevisiae URA3 terminator"
                     /note="Saccharomyces cerevisiae URA3 terminator"
                     /regulatory_class="terminator"
     CDS             1598..2407
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     promoter        complement(2712..2730)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2988..3576)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3750..4607)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4608..4712)
                     /label="AmpR promoter"
     promoter        5058..5074
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcagaacc aatgcattgg tttttccttt
       61 cctttccttt tctaattttc actcctggtt ttctttcttt cttagaaaca ttatctcgat
      121 attaatatta aaaaaatata atcattcaaa atgggcgtcg ctgacctcat caagaagttc
      181 gagtccatct ccaaggagga ggccggaggt gctggcgcag gtgcttctaa aggtgaagaa
      241 ttattcactg gtgttgtccc aattttggtt gaattagatg gtgatgttaa tggtcacaaa
      301 ttttctgtct ccggtgaagg tgaaggtgat gctacttacg gtaaattgac cttaaaattt
      361 atttgtacta ctggtaaatt gccagttcca tggccaacct tagtcactac tttatgttat
      421 ggtgttcaat gtttttcaag atacccagat catatgaaac aacatgactt tttcaagtct
      481 gccatgccag aaggttatgt tcaagaaaga actatttttt tcaaagatga cggtaactac
      541 aagaccagag ctgaagtcaa gtttgaaggt gataccttag ttaatagaat cgaattaaaa
      601 ggtattgatt ttaaagaaga tggtaacatt ttaggtcaca aattggaata caactataac
      661 tctcacaatg tttacatcat ggctgacaaa caaaagaatg gtatcaaagc taacttcaaa
      721 actagacaca acattgaaga tggttctgtt caattagctg accattatca acaaaatact
      781 ccaattggtg atggtccagt cttgttacca gacaaccatt acttatccac tcaatctgcc
      841 ttatccaaag atccaaacga aaagagagac cacatggtct tgttagaatt tgttactgct
      901 gctggtatta cccatggtat ggatgaattg tacaaataag gcgcgccatt ataagtaaat
      961 gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt attacccggg
     1021 aatctcggtc gtaatgattt ctataatgac gaaaaaaaaa aaattggaaa gaaaaagctt
     1081 catggccttt ataaaaagga actatccaat acctcgccag aaccaagtaa cagtatttta
     1141 cggggcacaa atcaagaaca ataagacagg actgtaaaga tggacgcata gatccggatg
     1201 gtataaacgg aaacaaaaaa aagagctggt actactttct ttaaaattat tttattattt
     1261 gattttattt aatagtatat attatatttt gaacgtagat tattttgttg aaagttgctg
     1321 tagtgccatt gattcgtaac actaattctg tattagtcat tcctcttgtt tgatagtatc
     1381 caaaaaaacg gctatttttt tgcaatctta tttcctgcat attatacaga taacataatg
     1441 aaagaaaaaa tctttttttt tgttcttcaa tgatgatttc aaccattctt ttaaacattg
     1501 atcaattcct gagcaacaac cccatacaca ctggtttata taccgcccct tttacagttg
     1561 aagaaagaaa tagaaataga aatagcaaac aaaagatatg acagtcaaca ctaagaccta
     1621 tagtgagaga gcagaaactc atgcctcacc agtagcacag cgattatttc gattaatgga
     1681 actgaagaaa accaatttat gtgcatcaat tgacgttgat accactaagg aattccttga
     1741 attaattgat aaattaggtc cttatgtatg cttaatcaag actcatattg atataatcaa
     1801 tgatttttcc tatgaatcca ctattgaacc attattagaa ctttcacgta aacatcaatt
     1861 tatgattttt gaagatagaa aatttgctga tattggtaat accgtaaaga aacaatatat
     1921 tggtggagtt tataaaatta gtagttgggc agatattacc aatgctcatg gtgtcactgg
     1981 gaatggagtg gttgaaggat taaaacaggg agctaaagaa accaccacca accaagagcc
     2041 aagagggtta ttgatgttag ctgaattatc atcagtggga tcattagcat atggagaata
     2101 ttctcaaaaa actgttgaaa ttgctaaatc cgataaggaa tttgttattg gatttattgc
     2161 ccaacgtgat atgggtggcc aagaagaagg atttgattgg cttattatga cacctggagt
     2221 tggattagat gataaaggtg atggattagg acaacaatat agaactgttg atgaagttgt
     2281 tagcactgga actgatatta tcattgttgg tagaggattg tttggtaaag gaagagatcc
     2341 agatattgaa ggtaaaaggt atagaaatgc tggttggaat gcttatttga aaaagactgg
     2401 ccaattataa atgtgaaggg ggagattttc actttattag atttgtatat atgtagaata
     2461 aataaataaa taagttaaat aaataattaa ataagggtgg taattattac tatttacaat
     2521 caaaggtggt ccttctagtt taaacgagct cgaattcatc gatgatatca gattctatct
     2581 tggcttcatt gtctactttc caacaaatgt ggatttcaaa acaagaatac gacgaatctg
     2641 gtccatccat tgttcaccac aaatgtttct aatccactag tggcctatgc ggccgcggat
     2701 ctgccggtct ccctatagtg agtcgtatta atttcgataa gccaggttaa cctgcattaa
     2761 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2821 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2881 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2941 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3001 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3061 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3121 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3181 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3241 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3301 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3361 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3421 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3481 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3541 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3601 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3661 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3721 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     4021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     4081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     4141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     4201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4381 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     4741 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     4801 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     4861 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     4921 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     4981 agagtgcacc atatggacat attgtcgtta gaacgcggct acaattaata cataacctta
     5041 tgtatcatac acatacgatt taggtgacac tata
//
