LOCUS       V015877                 3893 bp    DNA     circular SYN 17-JUL-2019
DEFINITION  Exported.
ACCESSION   V015877
VERSION     V015877
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     A new toolkit for gene tagging in Candida albicans containing
            recyclable markers
  JOURNAL   PLoS ONE (2019) In press
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Instituto de Biologia Funcional y Genomica
            (IBFG), Consejo Superior de Investigaciones Cientificas, Zacarias
            Gonzalez 2, Salamanca, Salamanca 37007, Spain
REFERENCE   3  (bases 1 to 3893)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 13
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE
            (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAR-2019) Instituto de Biologia Funcional y Genomica (IBFG),
            Consejo Superior de Investigaciones Cientificas, Zacarias Gonzalez
            2, Salamanca, Salamanca 37007, Spain"
FEATURES             Location/Qualifiers
     source          1..3893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     53..69
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    83..116
                     /label="loxP"
                     /note="loxP"
     CDS             464..1357
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     protein_bind    complement(1400..1433)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(1531..1549)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1807..2395)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2569..3426)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3427..3531)
                     /label="AmpR promoter"
     promoter        3877..3893
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acgggcggcc gctctagaac
       61 tagtggatcc ccatggtgat ccataacttc gtatagcata cattatacga agttatagat
      121 ccggtacctg gaggatgagg agacagaagt tagtagtaac aatttgggga cagaagagga
      181 tacagtgaca tcagaggaag aagacagcga aggtatagtc aaaaagcaat aggacagcaa
      241 gacaatttat ttatttaata gttttttata aatgtaaaat ctatcacaac atgtgtactt
      301 aattttctgt ggtaggggag gccatttcgt tgaaacgtgt cgcgagcggt tgtgagaatt
      361 gactcatttt gattattacg caaccattga aattttttat ttttttttgg tgaagatttt
      421 tcccacacaa cttcttcttt tacttaacca ccaactaccg ataatggatt tagtcaatca
      481 tttaccagac cgtttgttat ttgctgttcc taaaaagggc agattatacg aaaaatgctg
      541 taacttattg agtggtgccg atatacagtt tagaagatct aatagattag atatagcact
      601 ttctacaaac ttgccaattg cattaatctt cttgcctgca gctgatatcc cagttttcgt
      661 tggagaaggt aattgtgact tgggtataac tgggttagac caaatcaaag aagctgaaca
      721 attcgacaac atcgaggact tgttggattt gaaatttggt tcatgcaaat tgcagatcca
      781 agttccagca gatggcgagt acgaaaagcc agaacagctt gttggaaaga aaattgtgtc
      841 ttcatttaca aaattgagta ccgactattt caaacaattg tcagacaaac ctactaatat
      901 cagatatgtc ggtggttccg ttgaggcttc ttgtgccttg ggtgttgctg atgctattgt
      961 cgatttggtt gaaagtggtg aaactatgaa agcagctggg ttaaaggcca tagaaaccat
     1021 attggagact tcggctcatt tgatttcgtc aaagaaaagt aaattcccag aaatggtcaa
     1081 tataatcgtc caaagacttc aaggtgtttt agctgcccag gaatatgtct tgtgtaacta
     1141 caatgcccca aaatccatcc aagcaaaatg tttaaccatt actccaggca gaagggccgc
     1201 tactgtctct actttggaca aacacagcga cgacgaagag gactgggttg ccatctcatc
     1261 catggttaat agaaaggaaa tcggtaatgt aatggacgaa ttgaagaaag ctggtgcaac
     1321 cgatatattg gtactcgaga tatcaaattg tagagtttaa aagaagtgat agtttctcat
     1381 aaatattgct agcccatgga taacttcgta tagcatacat tatacgaagt tattagggat
     1441 aacagggtaa tctgcaggaa aaacgagctc gaattcatcg atgatatcag atccactagt
     1501 ggcctatgcg gccgcggatc tgccggtctc cctatagtga gtcgtattaa tttcgataag
     1561 ccaggttaac ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     1621 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1681 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1741 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1801 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1861 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1921 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1981 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2041 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2101 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2161 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2221 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     2281 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2341 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2401 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2461 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2521 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     2581 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     2641 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     2701 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     2761 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     2821 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     2881 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     2941 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     3001 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     3061 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3121 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3181 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3241 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     3301 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     3361 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     3421 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     3481 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     3541 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     3601 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     3661 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     3721 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     3781 atcagagcag attgtactga gagtgcacca tatggacata ttgtcgttag aacgcggcta
     3841 caattaatac ataaccttat gtatcataca catacgattt aggtgacact ata
//
