LOCUS       62056_10590        4599 bp DNA     circular SYN 17-JUL-2019
DEFINITION  Cloning vector pFA-LAL, complete sequence.
ACCESSION   MK652110
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F, 
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     A new toolkit for gene tagging in Candida albicans containing 
            recyclable markers
  JOURNAL   PLoS ONE (2019) In press
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F, 
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Instituto de Biologia Funcional y Genomica 
            (IBFG), Consejo Superior de Investigaciones Cientificas, Zacarias 
            Gonzalez 2, Salamanca, Salamanca 37007, Spain
REFERENCE   3  (bases 1 to 4599)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE 
            (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-MAR-2019) Instituto de Biologia Funcional y Genomica (IBFG), 
            Consejo Superior de Investigaciones Cientificas, Zacarias Gonzalez 
            2, Salamanca, Salamanca 37007, Spain"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 13
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     53..69
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    71..104
                     /label=loxP
                     /note="loxP"
     gene            105..2105
                     /gene="ARG4"
                     /label=ARG4
     CDS             571..1977
                     /codon_start=1
                     /transl_table=11
                     /gene="ARG4"
                     /product="ARG4"
                     /label=ARG4
                     /note="argininosuccinate lyase; derived from Candida
                     albicans ARG4"
                     /protein_id="QDK59742.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELHLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF
                     DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG
                     ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDAIGGTAKSAVLK
                     QLENLKSILS"
     protein_bind    complement(2106..2139)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(2237..2255)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2513..3101)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3275..4132)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4133..4237)
                     /label=AmpR promoter
     promoter        4583..4599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acgggcggcc gctctagaac
       61 tagtggatcc ataacttcgt atagcataca ttatacgaag ttatagatcc gtacccccct
      121 ttagtaagat ttttcaagag tagtctcaaa taaacagaat gaaggattga aggttttatt
      181 caataaataa ttggacagaa agttattgta cagttttaac aacaacaatt tcaaaatttc
      241 aatttatgac atattgaccg acataattca gtcatttcta atattcaaat aacaagacac
      301 cttgtataaa tgaatccaca atcgtatatg aacaaccaaa ttttatgact aactttgggt
      361 cgtgtacttt tgacgattga gtcattttta ccaattaggt aattgaattt tgttaatttg
      421 tcattaaccg cgctggccat cccaacaata acaacaatca atttaaaaac ttgacaaggc
      481 aaaaaaaaaa aaattttatc tttgaaaaat ttttcttcct tttcttcttc tgttctttac
      541 ataacagcta tcaagaataa tcaattaata atgtcacaac aacaagataa acaacctagt
      601 gaaaataaat tatggggtgg tcgtttcacc ggtgccactg atccattgat ggatttatat
      661 aatgcttcat taccttatga taaagttatg tatgatgctg atttaactgg tactaaagta
      721 tacacacaag gattaaacaa attaggatta attaccactg aagaattgca tttgattcat
      781 caaggattag aacaaatcag acaagaatgg catgataata aattcattat taaggcaggt
      841 gatgaggata ttcatactgc taatgaacgt cgattaggag aaatcattgg gaaaaacatt
      901 tctggtaaag ttcacactgg tagatcaaga aatgatcaag ttgctaccga tatgagaatt
      961 ttcgttcgtg aatcattact taatttaagt aaaattttac atcaattcat cacggcaatt
     1021 cttgaacgag cacataaaga aatcgatgta ttaatgcctg gttatactca tttacaaaaa
     1081 gctcaaccta ttagatgggc ccattggtta agttcatatg caacttattt caccgaagat
     1141 tataaacgtt tacaagaaat tataactcgt gttaaccaat ctccattagg atctggtgct
     1201 ttagcaggtc atccttatgg tattgatcgt gaatttttag ctaaaggatt aggatttgat
     1261 ggtgtcattg ggaattcttt aactgcagtt tcagatcgtg attttgttgt tgaaagttta
     1321 ttttggagta cattatttat gaatcatatt tctcgattct cagaagatct aattatttat
     1381 tctagtggtg aatttgggtt cataaaatta gctgatgctt attctactgg ttcttcattg
     1441 atgccacaaa agaaaaaccc tgactcatta gaattattac gtgggaaaag tggtagagta
     1501 tttggtcagt tatctggatt tttaatgtcg atcaaatcta ttccatctac ttataataaa
     1561 gatatgcaag aggataaaga accattattt gatgcattga ctacagtgga acattcaatt
     1621 ttaattgcta ctggtgtgat ttctacttta ctgattgata aacaaaatat ggaaaaagct
     1681 ttaactatgg atatgttggc tactgattta gctgattatt tagttagaaa aggagttcca
     1741 tttagagaaa ctcatcatat atctggtgaa tgtgttagaa aagctgaaga agaaaaatta
     1801 tctggaattg atcaattatc ttttgaacaa tttcaacaaa ttgattctcg ttttgaaaaa
     1861 gacgttatgg aaacttttga ttttgaagct agtgtggaaa gaagagatgc tattggtggt
     1921 actgctaaaa gtgccgtttt aaaacaatta gaaaatttaa aatcaatttt aagttaattt
     1981 ataaatagtc atataataat cacagtattg tttaacttac ataaaatata atcaattaac
     2041 acagagatac cttgtactcc aatactagca ttccaaccag catttctata cctgctagcc
     2101 catggataac ttcgtatagc atacattata cgaagttatt agggataaca gggtaatctg
     2161 caggaaaaac gagctcgaat tcatcgatga tatcagatcc actagtggcc tatgcggccg
     2221 cggatctgcc ggtctcccta tagtgagtcg tattaatttc gataagccag gttaacctgc
     2281 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     2341 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2401 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2461 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2521 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2581 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2641 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2701 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2761 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2821 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2881 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2941 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3001 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3061 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3121 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3181 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3241 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3301 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3361 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3421 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3481 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3541 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3601 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3661 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3721 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3781 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3841 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3901 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3961 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     4021 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4081 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4141 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4201 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4261 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     4321 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     4381 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     4441 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     4501 tactgagagt gcaccatatg gacatattgt cgttagaacg cggctacaat taatacataa
     4561 ccttatgtat catacacata cgatttaggt gacactata
//
