LOCUS       40924_19106        6162 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Functional Analysis vector pFA-HAH1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Paul S, Ganesan K.
  TITLE     Single-Transformation Gene Function Test by Conditional Expression 
            of Both Alleles of Candida albicans Genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Paul S, Ganesan K.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2010) Institute of Microbial Technology, Sector 
            39-A, Chandigarh, UT 160036, India
REFERENCE   3  (bases 1 to 6162)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6162)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-NOV-2010) Institute of Microbial Technology, Sector 39-A, 
            Chandigarh, UT 160036, India"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            47..915
                     /gene="HIS1"
                     /label=HIS1
     repeat_region   562..915
     gene            921..2871
                     /gene="ARG4"
                     /label=ARG4
                     /note="derived from Candida albicans ARG4"
     CDS             1378..2784
                     /codon_start=1
                     /gene="ARG4"
                     /product="argininosuccinate lyase"
                     /EC_number="4.3.2.1"
                     /label=ARG4
                     /protein_id="AEQ75399.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELNLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF
                     DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG
                     ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDALGGTAKSAVLK
                     QLENLKSILS"
     gene            2876..3729
                     /gene="HIS1"
                     /label=HIS1
     repeat_region   2876..3229
     promoter        complement(3800..3818)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4076..4664)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4838..5695)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5696..5800)
                     /label=AmpR promoter
     promoter        6146..6162
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcctg gaggatgagg
       61 agacagaagt tagtagtaac aatttgggga cagaagagga tacagtgaca tcagaggaag
      121 aagacagcga aggtatagtc aaaaagcaat aggacagcaa gacaatttat ttatttaata
      181 gttttttata aatgtaaaat ctatcacaac atgtgtactt aattttctgt ggtaggggag
      241 gccatttcgt tgaaacgtgt cgcgagcggt tgtgagaatt gactcatttt gattattacg
      301 caaccattga aattttttat ttttttttgg tgaagatttt tcccacacaa cttcttcttt
      361 tacttaacca ccaactaccg ataatggatt tagtcaatca tttaccagac cgtttgttat
      421 ttgctgttcc taaaaagggc agattatacg aaaaatgctg taacttattg agtggtgccg
      481 atatacagtt tagaagatct aatagattag atatagcact ttctacaaac ttgccaattg
      541 cattaatctt cttgcctgca gctgatatcc cagttttcgt tggagaaggt aattgtgact
      601 tgggtataac tgggttagac caaatcaaag aagctgaaca attcgacaac atcgaggact
      661 tgttggattt gaaatttggt tcatgcaaat tgcagatcca agttccagca gatggcgagt
      721 acgaaaagcc agaacagctt gttggaaaga aaattgtgtc ttcatttaca aaattgagta
      781 ccgactattt caaacaattg tcagacaaac ctactaatat cagatatgtc ggtggttccg
      841 ttgaggcttc ttgtgccttg ggtgttgctg atgctattgt cgatttggtt gaaagtggtg
      901 aaactatgaa agcaggatcc cccctttagt aagatttttc aagagtagtc tcaaataaac
      961 agaatgaagg attgaaggtt ttattcaata aataattgga cagaaagtta ttgcacagtt
     1021 ttaacaacaa caatttcaaa atttcaattt atgacatatt gaccgacata attcagtcat
     1081 ttctaatatt caaataacaa gacaccttgt ataaatgaat ccacaagcgt atatgaacaa
     1141 ccaaatttta tgactaactg tgggtcgtgt acttttgacg attgagtcat ttttaccaat
     1201 taggtaattg aattttacta atttgtcatt aaccgcgctg gccatcccaa caacaacaac
     1261 aacaatcaat ttaaaaactt gacaaggcaa aaaaaaaaaa tttcatcttt gaaaaatttt
     1321 tcttcctttt cttcttctgt tctttacata acagctatca agaataatca attaataatg
     1381 tcacaacaac aagataaaca acctagtgaa aataaattat ggggtggtcg tttcaccggt
     1441 gccactgatc cattgatgga tttgtataat gcttcattac cttatgataa agttatgtat
     1501 gatgctgatt taactggtac taaagtatac acacaaggat taaacaaatt aggattaatt
     1561 accactgaag aattgaattt gattcatcaa ggattagaac aaatcagaca agaatggcat
     1621 gataataaat tcattattaa ggctggtgat gaggatattc atactgctaa tgaacgtcga
     1681 ttaggagaaa tcattgggaa aaacatttct ggtaaagttc acactggtag atcaagaaat
     1741 gatcaagttg ctaccgatat gagaattttc gttcgtgaat cattacttaa tttaagtaaa
     1801 attttacatc aattcatcac ggcaattctt gaacgagcac ataaagaaat cgatgtatta
     1861 atgcctggtt atactcattt acaaaaagct caacctatta gatgggccca ttggttaagt
     1921 tcatatgcaa cttatttcac cgaagattat aaacgtttac aagaaattat aactcgtgtt
     1981 aaccaatctc cattaggatc tggtgcttta gcaggtcatc cttatggtat tgatcgtgaa
     2041 tttttagcta aaggattagg atttgatggt gtcattggga attctttaac tgcagtttca
     2101 gatcgtgatt ttgttgttga aagtttattt tggagtacat tatttatgaa tcatatttct
     2161 cgattctcag aagatctaat tatttattct agtggtgaat ttgggttcat aaaattagct
     2221 gatgcttatt ctactggttc ttcattgatg ccacaaaaga aaaaccctga ctcattagaa
     2281 ttattacgtg ggaaaagtgg tagagtattt ggtcagttat ctggattttt aatgtcgatc
     2341 aaatctattc catctactta taataaagat atgcaagagg ataaagaacc attatttgat
     2401 gcattgacta cagtagaaca ttcaatttta attgctactg gtgtgatttc tactttactg
     2461 attgataaac aaaatatgga aaaagcttta actatggata tgttggctac tgatttagct
     2521 gattatttag ttagaaaagg agttccattt agagaaactc atcatatatc tggtgaatgt
     2581 gttagaaaag ctgaagaaga aaaattatct ggaattgatc aattatcttt tgaacaattt
     2641 caacaaattg attctcgttt tgaaaaagac gttatggaaa cttttgattt tgaagctagt
     2701 gtggaaagaa gagatgctct tggtggtact gctaaaagtg ccgttttaaa acaattagaa
     2761 aatttaaaat caatcttaag ttaatttata aatagtcata taataatcac agtattgttt
     2821 aacttacata aaatataatc aattaacaca gagatacctt gtactacaat agtttctgat
     2881 atcccagttt tcgttggaga aggtaattgt gacttgggta taactgggtt agaccaaatc
     2941 aaagaagctg aacaattcga caacatcgag gacttgttgg atttgaaatt tggttcatgc
     3001 aaattgcaga tccaagttcc agcagatggc gagtacgaaa agccagaaca gcttgttgga
     3061 aagaaaattg tgtcttcatt tacaaaattg agtaccgact atttcaaaca attgtcagac
     3121 aaacctacta atatcagata tgtcggtggt tccgttgagg cttcttgtgc cttgggtgtt
     3181 gctgatgcta ttgtcgattt ggttgaaagt ggtgaaacta tgaaagcagc tgggttaaag
     3241 gccatagaaa ccatattgga gacttcggct catttgattt cgtcaaaaaa aagtaaattc
     3301 ccagaaatgg tcaatataat cgtccaaaga cttcaaggtg ttttagctgc ccaggaatat
     3361 gtcttgtgta actacaatgc cccaaaatcc atccaagcaa aatgtttaac cattactcca
     3421 ggcagaaggg ccgctactgt ctctactttg gacaaacaca gcgacgacga agaggactgg
     3481 gttgccatct catccatggt taatagaaag gaaatcggta atgtaatgga cgaattgaag
     3541 aaagctggtg caaccgatat attggtactc gagatatcaa attgtagagt ttaaaagaag
     3601 tgatagtttc tcataaatat tatacatata tacttataga tctacttcaa tttgactcaa
     3661 tctgtcttat accatcttta tctaccacct tgatgtacac acctttaaag tcaataggca
     3721 ttctcgttta aacgagctcg aattcatcga tgatatcaga tccactagtg gcctatgcgg
     3781 ccgcggatct gccggtctcc ctatagtgag tcgtattaat ttcgataagc caggttaacc
     3841 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     3901 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3961 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4021 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4081 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4141 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4201 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4261 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4321 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4381 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4441 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4501 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4561 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4621 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4681 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4741 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4801 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4861 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4921 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4981 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5041 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5101 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5161 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     5221 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5281 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5341 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5401 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     5461 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5521 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5581 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5641 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5701 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5761 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5821 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     5881 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     5941 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     6001 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     6061 ttgtactgag agtgcaccat atggacatat tgtcgttaga acgcggctac aattaataca
     6121 taaccttatg tatcatacac atacgattta ggtgacacta ta
//
