LOCUS       40924_19101        7531 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Functional Analysis vector pFA-HAH1-MET3prom, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7531)
  AUTHORS   Paul S, Ganesan K.
  TITLE     Single-Transformation Gene Function Test by Conditional Expression 
            of Both Alleles of Candida albicans Genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7531)
  AUTHORS   Paul S, Ganesan K.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2010) Institute of Microbial Technology, Sector 
            39-A, Chandigarh, UT 160036, India
REFERENCE   3  (bases 1 to 7531)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7531)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-NOV-2010) Institute of Microbial Technology, Sector 39-A, 
            Chandigarh, UT 160036, India"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            47..915
                     /gene="HIS1"
                     /label=HIS1
     repeat_region   562..915
     gene            921..2871
                     /gene="ARG4"
                     /label=ARG4
                     /note="derived from Candida albicans ARG4"
     CDS             1378..2784
                     /codon_start=1
                     /gene="ARG4"
                     /product="argininosuccinate lyase"
                     /EC_number="4.3.2.1"
                     /label=ARG4
                     /protein_id="AEQ75403.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELNLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF
                     DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG
                     ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDALGGTAKSAVLK
                     QLENLKSILS"
     gene            2876..3729
                     /gene="HIS1"
                     /label=HIS1
     repeat_region   2876..3229
     regulatory      3734..5132
                     /label=MET3 promoter from Candida albicans
                     /note="MET3 promoter from Candida albicans"
                     /regulatory_class="promoter"
     promoter        complement(5169..5187)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(5445..6033)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6207..7064)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7065..7169)
                     /label=AmpR promoter
     promoter        7515..7531
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcctg gaggatgagg
       61 agacagaagt tagtagtaac aatttgggga cagaagagga tacagtgaca tcagaggaag
      121 aagacagcga aggtatagtc aaaaagcaat aggacagcaa gacaatttat ttatttaata
      181 gttttttata aatgtaaaat ctatcacaac atgtgtactt aattttctgt ggtaggggag
      241 gccatttcgt tgaaacgtgt cgcgagcggt tgtgagaatt gactcatttt gattattacg
      301 caaccattga aattttttat ttttttttgg tgaagatttt tcccacacaa cttcttcttt
      361 tacttaacca ccaactaccg ataatggatt tagtcaatca tttaccagac cgtttgttat
      421 ttgctgttcc taaaaagggc agattatacg aaaaatgctg taacttattg agtggtgccg
      481 atatacagtt tagaagatct aatagattag atatagcact ttctacaaac ttgccaattg
      541 cattaatctt cttgcctgca gctgatatcc cagttttcgt tggagaaggt aattgtgact
      601 tgggtataac tgggttagac caaatcaaag aagctgaaca attcgacaac atcgaggact
      661 tgttggattt gaaatttggt tcatgcaaat tgcagatcca agttccagca gatggcgagt
      721 acgaaaagcc agaacagctt gttggaaaga aaattgtgtc ttcatttaca aaattgagta
      781 ccgactattt caaacaattg tcagacaaac ctactaatat cagatatgtc ggtggttccg
      841 ttgaggcttc ttgtgccttg ggtgttgctg atgctattgt cgatttggtt gaaagtggtg
      901 aaactatgaa agcaggatcc cccctttagt aagatttttc aagagtagtc tcaaataaac
      961 agaatgaagg attgaaggtt ttattcaata aataattgga cagaaagtta ttgcacagtt
     1021 ttaacaacaa caatttcaaa atttcaattt atgacatatt gaccgacata attcagtcat
     1081 ttctaatatt caaataacaa gacaccttgt ataaatgaat ccacaagcgt atatgaacaa
     1141 ccaaatttta tgactaactg tgggtcgtgt acttttgacg attgagtcat ttttaccaat
     1201 taggtaattg aattttacta atttgtcatt aaccgcgctg gccatcccaa caacaacaac
     1261 aacaatcaat ttaaaaactt gacaaggcaa aaaaaaaaaa tttcatcttt gaaaaatttt
     1321 tcttcctttt cttcttctgt tctttacata acagctatca agaataatca attaataatg
     1381 tcacaacaac aagataaaca acctagtgaa aataaattat ggggtggtcg tttcaccggt
     1441 gccactgatc cattgatgga tttgtataat gcttcattac cttatgataa agttatgtat
     1501 gatgctgatt taactggtac taaagtatac acacaaggat taaacaaatt aggattaatt
     1561 accactgaag aattgaattt gattcatcaa ggattagaac aaatcagaca agaatggcat
     1621 gataataaat tcattattaa ggctggtgat gaggatattc atactgctaa tgaacgtcga
     1681 ttaggagaaa tcattgggaa aaacatttct ggtaaagttc acactggtag atcaagaaat
     1741 gatcaagttg ctaccgatat gagaattttc gttcgtgaat cattacttaa tttaagtaaa
     1801 attttacatc aattcatcac ggcaattctt gaacgagcac ataaagaaat cgatgtatta
     1861 atgcctggtt atactcattt acaaaaagct caacctatta gatgggccca ttggttaagt
     1921 tcatatgcaa cttatttcac cgaagattat aaacgtttac aagaaattat aactcgtgtt
     1981 aaccaatctc cattaggatc tggtgcttta gcaggtcatc cttatggtat tgatcgtgaa
     2041 tttttagcta aaggattagg atttgatggt gtcattggga attctttaac tgcagtttca
     2101 gatcgtgatt ttgttgttga aagtttattt tggagtacat tatttatgaa tcatatttct
     2161 cgattctcag aagatctaat tatttattct agtggtgaat ttgggttcat aaaattagct
     2221 gatgcttatt ctactggttc ttcattgatg ccacaaaaga aaaaccctga ctcattagaa
     2281 ttattacgtg ggaaaagtgg tagagtattt ggtcagttat ctggattttt aatgtcgatc
     2341 aaatctattc catctactta taataaagat atgcaagagg ataaagaacc attatttgat
     2401 gcattgacta cagtagaaca ttcaatttta attgctactg gtgtgatttc tactttactg
     2461 attgataaac aaaatatgga aaaagcttta actatggata tgttggctac tgatttagct
     2521 gattatttag ttagaaaagg agttccattt agagaaactc atcatatatc tggtgaatgt
     2581 gttagaaaag ctgaagaaga aaaattatct ggaattgatc aattatcttt tgaacaattt
     2641 caacaaattg attctcgttt tgaaaaagac gttatggaaa cttttgattt tgaagctagt
     2701 gtggaaagaa gagatgctct tggtggtact gctaaaagtg ccgttttaaa acaattagaa
     2761 aatttaaaat caatcttaag ttaatttata aatagtcata taataatcac agtattgttt
     2821 aacttacata aaatataatc aattaacaca gagatacctt gtactacaat agtttctgat
     2881 atcccagttt tcgttggaga aggtaattgt gacttgggta taactgggtt agaccaaatc
     2941 aaagaagctg aacaattcga caacatcgag gacttgttgg atttgaaatt tggttcatgc
     3001 aaattgcaga tccaagttcc agcagatggc gagtacgaaa agccagaaca gcttgttgga
     3061 aagaaaattg tgtcttcatt tacaaaattg agtaccgact atttcaaaca attgtcagac
     3121 aaacctacta atatcagata tgtcggtggt tccgttgagg cttcttgtgc cttgggtgtt
     3181 gctgatgcta ttgtcgattt ggttgaaagt ggtgaaacta tgaaagcagc tgggttaaag
     3241 gccatagaaa ccatattgga gacttcggct catttgattt cgtcaaaaaa aagtaaattc
     3301 ccagaaatgg tcaatataat cgtccaaaga cttcaaggtg ttttagctgc ccaggaatat
     3361 gtcttgtgta actacaatgc cccaaaatcc atccaagcaa aatgtttaac cattactcca
     3421 ggcagaaggg ccgctactgt ctctactttg gacaaacaca gcgacgacga agaggactgg
     3481 gttgccatct catccatggt taatagaaag gaaatcggta atgtaatgga cgaattgaag
     3541 aaagctggtg caaccgatat attggtactc gagatatcaa attgtagagt ttaaaagaag
     3601 tgatagtttc tcataaatat tatacatata tacttataga tctacttcaa tttgactcaa
     3661 tctgtcttat accatcttta tctaccacct tgatgtacac acctttaaag tcaataggca
     3721 ttctcgttta aacaatgact attggtagcg tgttctattt ttcttatgcg ctgaaaaact
     3781 acgaacaatt gtctattcca agcctgtggc tttttttggt gattcacaat acataaattg
     3841 gcaaatacct cttctataga ctttaacctt tgatgaaatg gtatggataa accacttttg
     3901 tggtcagggt atatatattt ttattccaat ctggccttta taaattcaaa tttccaaggg
     3961 gactctgcta aaccttctga tataactata actctaataa tggctgtaaa aagtgtgaaa
     4021 aaaagatgat attcacaaaa aagataaagg ggatttcata ttgggtatct agatttaaac
     4081 atcgtatttc aacaatgaaa ctagaatcaa tacgaaatcc agtgcacaac tgtgttcatt
     4141 cccttgggaa ttatactgag taatgggaag aatgtgcaaa aaatacccgt gtcttatata
     4201 atttccattt gtgtagggct taaaagtgga caacttcagg ttacgaaacc acgaaaatgt
     4261 ctaaacgttt gatcaaattc gtaactcatg gaaaatcaca gcaacaaatt aaggatggat
     4321 atatccggta cttacaaata aaaacactaa agaagtataa attttgaact gaaattctat
     4381 agaaaatctt ttttttttgg gtaaaattat gcgattgtgg ctcatagtaa cgtaatcata
     4441 cagttcaaca aacatcattt tgtcaaagga tttctaatcc cattcttttt ctacttggct
     4501 tttgagtata tatatatata catcctttgt tcatcatcat gttcgattga atttttttct
     4561 tggttgttat tagattgtct acttgagttt ttcttggtgc aaattgtggt gtttttctaa
     4621 caaatttttc aacatttaaa gtatttctgg aacttttcta caaggaacga catagatcca
     4681 ttaatgcgcc cctctaaaac aatacccata atgtcttctt aagtatgcat ctgcttataa
     4741 atgtttctgg atttacttta gttagaatgt cgacacgaca atgaactatt gctgtggatc
     4801 acgtgcgatc actgaattat gagtttcttt ttatttttct ttttgggctt ttttttgcaa
     4861 tttttttttt ccacctttgc taattgcgta gtcacatata atgtatacaa tttttgcaac
     4921 tctgacacta taattgatcc aataataaag tatataatag atcatcaagt atacgtaatc
     4981 tccccaaaaa aaaaattgca taaaataaaa tctataaacc ttttttagat ttttagttgt
     5041 tccttttttc tttctattct tcaacttcca agtgttgtca ctttcttcct gttaactata
     5101 tttaaaagta aataccctcc ccagaaaaca tgactagtgg cctatgcggc cgcggatctg
     5161 ccggtctccc tatagtgagt cgtattaatt tcgataagcc aggttaacct gcattaatga
     5221 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     5281 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5341 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     5401 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5461 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5521 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5581 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     5641 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5701 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5761 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5821 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     5881 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     5941 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6001 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6061 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6121 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6181 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6241 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6301 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6361 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     6421 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6481 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     6541 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     6601 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     6661 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     6721 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     6781 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     6841 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     6901 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     6961 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7021 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7081 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7141 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     7201 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     7261 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     7321 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     7381 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     7441 gtgcaccata tggacatatt gtcgttagaa cgcggctaca attaatacat aaccttatgt
     7501 atcatacaca tacgatttag gtgacactat a
//
