LOCUS       40924_19096        6668 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Functional Analysis vector pFA-HAH1-MAL2prom, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Paul S, Ganesan K.
  TITLE     Single-Transformation Gene Function Test by Conditional Expression 
            of Both Alleles of Candida albicans Genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Paul S, Ganesan K.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2010) Institute of Microbial Technology, Sector 
            39-A, Chandigarh, UT 160036, India
REFERENCE   3  (bases 1 to 6668)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6668)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-NOV-2010) Institute of Microbial Technology, Sector 39-A, 
            Chandigarh, UT 160036, India"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            47..915
                     /gene="HIS1"
                     /label=HIS1
     repeat_region   562..915
     gene            921..2871
                     /gene="ARG4"
                     /label=ARG4
                     /note="derived from Candida albicans ARG4"
     CDS             1378..2784
                     /codon_start=1
                     /gene="ARG4"
                     /product="argininosuccinate lyase"
                     /EC_number="4.3.2.1"
                     /label=ARG4
                     /protein_id="AEQ75401.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELNLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF
                     DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG
                     ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDALGGTAKSAVLK
                     QLENLKSILS"
     gene            2876..3729
                     /gene="HIS1"
                     /label=HIS1
     repeat_region   2876..3229
     regulatory      3734..4269
                     /label=MAL2 promoter from Candida albicans
                     /note="MAL2 promoter from Candida albicans"
                     /regulatory_class="promoter"
     promoter        complement(4306..4324)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4582..5170)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5344..6201)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6202..6306)
                     /label=AmpR promoter
     promoter        6652..6668
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcctg gaggatgagg
       61 agacagaagt tagtagtaac aatttgggga cagaagagga tacagtgaca tcagaggaag
      121 aagacagcga aggtatagtc aaaaagcaat aggacagcaa gacaatttat ttatttaata
      181 gttttttata aatgtaaaat ctatcacaac atgtgtactt aattttctgt ggtaggggag
      241 gccatttcgt tgaaacgtgt cgcgagcggt tgtgagaatt gactcatttt gattattacg
      301 caaccattga aattttttat ttttttttgg tgaagatttt tcccacacaa cttcttcttt
      361 tacttaacca ccaactaccg ataatggatt tagtcaatca tttaccagac cgtttgttat
      421 ttgctgttcc taaaaagggc agattatacg aaaaatgctg taacttattg agtggtgccg
      481 atatacagtt tagaagatct aatagattag atatagcact ttctacaaac ttgccaattg
      541 cattaatctt cttgcctgca gctgatatcc cagttttcgt tggagaaggt aattgtgact
      601 tgggtataac tgggttagac caaatcaaag aagctgaaca attcgacaac atcgaggact
      661 tgttggattt gaaatttggt tcatgcaaat tgcagatcca agttccagca gatggcgagt
      721 acgaaaagcc agaacagctt gttggaaaga aaattgtgtc ttcatttaca aaattgagta
      781 ccgactattt caaacaattg tcagacaaac ctactaatat cagatatgtc ggtggttccg
      841 ttgaggcttc ttgtgccttg ggtgttgctg atgctattgt cgatttggtt gaaagtggtg
      901 aaactatgaa agcaggatcc cccctttagt aagatttttc aagagtagtc tcaaataaac
      961 agaatgaagg attgaaggtt ttattcaata aataattgga cagaaagtta ttgcacagtt
     1021 ttaacaacaa caatttcaaa atttcaattt atgacatatt gaccgacata attcagtcat
     1081 ttctaatatt caaataacaa gacaccttgt ataaatgaat ccacaagcgt atatgaacaa
     1141 ccaaatttta tgactaactg tgggtcgtgt acttttgacg attgagtcat ttttaccaat
     1201 taggtaattg aattttacta atttgtcatt aaccgcgctg gccatcccaa caacaacaac
     1261 aacaatcaat ttaaaaactt gacaaggcaa aaaaaaaaaa tttcatcttt gaaaaatttt
     1321 tcttcctttt cttcttctgt tctttacata acagctatca agaataatca attaataatg
     1381 tcacaacaac aagataaaca acctagtgaa aataaattat ggggtggtcg tttcaccggt
     1441 gccactgatc cattgatgga tttgtataat gcttcattac cttatgataa agttatgtat
     1501 gatgctgatt taactggtac taaagtatac acacaaggat taaacaaatt aggattaatt
     1561 accactgaag aattgaattt gattcatcaa ggattagaac aaatcagaca agaatggcat
     1621 gataataaat tcattattaa ggctggtgat gaggatattc atactgctaa tgaacgtcga
     1681 ttaggagaaa tcattgggaa aaacatttct ggtaaagttc acactggtag atcaagaaat
     1741 gatcaagttg ctaccgatat gagaattttc gttcgtgaat cattacttaa tttaagtaaa
     1801 attttacatc aattcatcac ggcaattctt gaacgagcac ataaagaaat cgatgtatta
     1861 atgcctggtt atactcattt acaaaaagct caacctatta gatgggccca ttggttaagt
     1921 tcatatgcaa cttatttcac cgaagattat aaacgtttac aagaaattat aactcgtgtt
     1981 aaccaatctc cattaggatc tggtgcttta gcaggtcatc cttatggtat tgatcgtgaa
     2041 tttttagcta aaggattagg atttgatggt gtcattggga attctttaac tgcagtttca
     2101 gatcgtgatt ttgttgttga aagtttattt tggagtacat tatttatgaa tcatatttct
     2161 cgattctcag aagatctaat tatttattct agtggtgaat ttgggttcat aaaattagct
     2221 gatgcttatt ctactggttc ttcattgatg ccacaaaaga aaaaccctga ctcattagaa
     2281 ttattacgtg ggaaaagtgg tagagtattt ggtcagttat ctggattttt aatgtcgatc
     2341 aaatctattc catctactta taataaagat atgcaagagg ataaagaacc attatttgat
     2401 gcattgacta cagtagaaca ttcaatttta attgctactg gtgtgatttc tactttactg
     2461 attgataaac aaaatatgga aaaagcttta actatggata tgttggctac tgatttagct
     2521 gattatttag ttagaaaagg agttccattt agagaaactc atcatatatc tggtgaatgt
     2581 gttagaaaag ctgaagaaga aaaattatct ggaattgatc aattatcttt tgaacaattt
     2641 caacaaattg attctcgttt tgaaaaagac gttatggaaa cttttgattt tgaagctagt
     2701 gtggaaagaa gagatgctct tggtggtact gctaaaagtg ccgttttaaa acaattagaa
     2761 aatttaaaat caatcttaag ttaatttata aatagtcata taataatcac agtattgttt
     2821 aacttacata aaatataatc aattaacaca gagatacctt gtactacaat agtttctgat
     2881 atcccagttt tcgttggaga aggtaattgt gacttgggta taactgggtt agaccaaatc
     2941 aaagaagctg aacaattcga caacatcgag gacttgttgg atttgaaatt tggttcatgc
     3001 aaattgcaga tccaagttcc agcagatggc gagtacgaaa agccagaaca gcttgttgga
     3061 aagaaaattg tgtcttcatt tacaaaattg agtaccgact atttcaaaca attgtcagac
     3121 aaacctacta atatcagata tgtcggtggt tccgttgagg cttcttgtgc cttgggtgtt
     3181 gctgatgcta ttgtcgattt ggttgaaagt ggtgaaacta tgaaagcagc tgggttaaag
     3241 gccatagaaa ccatattgga gacttcggct catttgattt cgtcaaaaaa aagtaaattc
     3301 ccagaaatgg tcaatataat cgtccaaaga cttcaaggtg ttttagctgc ccaggaatat
     3361 gtcttgtgta actacaatgc cccaaaatcc atccaagcaa aatgtttaac cattactcca
     3421 ggcagaaggg ccgctactgt ctctactttg gacaaacaca gcgacgacga agaggactgg
     3481 gttgccatct catccatggt taatagaaag gaaatcggta atgtaatgga cgaattgaag
     3541 aaagctggtg caaccgatat attggtactc gagatatcaa attgtagagt ttaaaagaag
     3601 tgatagtttc tcataaatat tatacatata tacttataga tctacttcaa tttgactcaa
     3661 tctgtcttat accatcttta tctaccacct tgatgtacac acctttaaag tcaataggca
     3721 ttctcgttta aacttttgtc tagtaccatc tgtaccatta cacttaaatt atctttatat
     3781 ctgtctaact cgactgtctg gatttcattg atgtagtcgt atgcatcgtt agttccaaaa
     3841 aatattgtca tcaatttgat attggtttcc gactctaaaa tttttggaag aatttgtcta
     3901 gcgtgctctg agttgtagcc actgaaacca cggttaataa catccaattt tcggatatac
     3961 acattctgta atgctggatg aaagccatac tgggtacaac taaactgggt gatggagtca
     4021 ccgaacaaca caaatttacc gtattccatg attgctatgg ttgagaattt tttttttttc
     4081 ttgtcccacg ccatttttca aattatgcag ttgagaatgt tagtttttgt gtacaccccg
     4141 ttcgctgaat atttcggaat aattcaaaga ttggggagtg ggggaggcga tagacgaaga
     4201 cacggtataa aaatgggcaa aattttcccc aactttttgc agtggtttaa ctaataatca
     4261 actacaatga ctagtggcct atgcggccgc ggatctgccg gtctccctat agtgagtcgt
     4321 attaatttcg ataagccagg ttaacctgca ttaatgaatc ggccaacgcg cggggagagg
     4381 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     4441 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     4501 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4561 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4621 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4681 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4741 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag
     4801 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4861 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4921 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4981 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     5041 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     5101 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     5161 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     5221 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     5281 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     5341 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     5401 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     5461 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     5521 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     5581 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     5641 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     5701 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     5761 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     5821 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5881 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     5941 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     6001 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     6061 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     6121 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     6181 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     6241 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     6301 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     6361 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     6421 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     6481 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     6541 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgg acatattgtc
     6601 gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg
     6661 acactata
//
