LOCUS       V015354                 4363 bp    DNA     circular SYN 17-JUL-2019
DEFINITION  Exported.
ACCESSION   V015354
VERSION     V015354
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     A new toolkit for gene tagging in Candida albicans containing
            recyclable markers
  JOURNAL   PLoS ONE (2019) In press
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Instituto de Biologia Funcional y Genomica
            (IBFG), Consejo Superior de Investigaciones Cientificas, Zacarias
            Gonzalez 2, Salamanca, Salamanca 37007, Spain
REFERENCE   3  (bases 1 to 4363)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 13
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE
            (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAR-2019) Instituto de Biologia Funcional y Genomica (IBFG),
            Consejo Superior de Investigaciones Cientificas, Zacarias Gonzalez
            2, Salamanca, Salamanca 37007, Spain"
FEATURES             Location/Qualifiers
     source          1..4363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             45..71
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             75..101
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             105..131
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     regulatory      135..390
                     /note="Transcriptional termination from Saccharomyces
                     cerevisiae URA3 gene"
                     /regulatory_class="terminator"
     primer_bind     400..416
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    430..463
                     /label="loxP"
                     /note="loxP"
     CDS             893..1702
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     protein_bind    complement(1853..1886)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(1984..2002)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2260..2848)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3022..3879)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3880..3984)
                     /label="AmpR promoter"
     promoter        4330..4348
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaagcttcgt acgctgcagg tcgacgggtc gacggatccc cgggtaccca tacgatgttc
       61 ctgactatgc gggctatccc tatgacgtcc cggactatgc aggatatcca tatgacgttc
      121 cagattacgc ttagggcgcg ccattataag taaatgcatg tatactaaac tcacaaatta
      181 gagcttcaat ttaattatat cagttattac ccgggaatct cggtcgtaat gatttctata
      241 atgacgaaaa aaaaaaaatt ggaaagaaaa agcttcatgg cctttataaa aaggaactat
      301 ccaatacctc gccagaacca agtaacagta ttttacgggg cacaaatcaa gaacaataag
      361 acaggactgt aaagatggac gcatagatcc ggcggccgct ctagaactag tggatccaga
      421 tcttgatcca taacttcgta tagcatacat tatacgaagt tatagatcca gtactaatag
      481 gaattgattt ggatggtata aacggaaaca aaaaaaagag ctggtactac tttctttaaa
      541 attattttat tatttgattt tatttaatag tatatattat attttgaacg tagattattt
      601 tgttgaaagt tgctgtagtg ccattgattc gtaacactaa ttctgtatta gtcattcctc
      661 ttgtttgata gtatccaaaa aaacggctat ttttttgcaa tcttatttcc tgcatattat
      721 acagataaca taatgaaaga aaaaatcttt ttttttgttc ttcaatgatg atttcaacca
      781 ttcttttaaa cattgatcaa ttcctgagca acaaccccat acacactggt ttatatatcg
      841 ccccttttac agttgaagaa agaaatagaa atagaaatag caaacaaaag atatgacagt
      901 caacactaag acctatagtg agagagcaga aactcatgcc tcaccagtag cacaacgatt
      961 atttcgatta atggaactga agaaaaccaa tttatgtgca tcaattgatg ttgataccac
     1021 taaggaattc cttgaattaa ttgataaatt gggtccttat gtatgcttaa tcaagactca
     1081 tattgatata atcaatgatt tttcctatga atccactatt gaaccattat tagaactttc
     1141 acgtaaacat caatttatga tttttgaaga tagaaaattt gctgatattg gtaataccgt
     1201 gaagaaacaa tatattggtg gagtttataa aattagtagt tgggcagata ttactaatgc
     1261 tcatggtgtc actgggaatg gagtagttga aggattaaaa cagggagcta aagaaaccac
     1321 caccaaccaa gagccaagag ggttattgat gttagctgaa ttatcatcag tgggatcatt
     1381 agcatatgga gaatattctc aaaaaactgt tgaaattgct aaatccgata aggaatttgt
     1441 tattggattt attgcccaac gtgatatggg tggacaagaa gaaggatttg attggcttat
     1501 tatgacacct ggagttggat tagatgataa aggtgatgga ttaggacaac aatatagaac
     1561 tgttgatgaa gttgttagca ctggaactga tattatcatt gttggtagag gattgtttgg
     1621 taaaggaaga gatccagata ttgaaggtaa aaggtataga gatgctggtt ggaatgctta
     1681 tttgaaaaaa actggccaat tataaatgtg aagggggaga ttttcacttt attagatttg
     1741 tatatatgta taataaataa ataaataagt taaataaata attaaataag ggtggtaatt
     1801 attactattt acaatcaaag gtggtccttc tagaagatct gctagcccat ggataacttc
     1861 gtatagcata cattatacga agttattagg gataacaggg taatctgcag gaaaaacgag
     1921 ctcgaattca tcgatgatat cagatccact agtggcctat gcggccgcgg atctgccggt
     1981 ctccctatag tgagtcgtat taatttcgat aagccaggtt aacctgcatt aatgaatcgg
     2041 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     2101 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2161 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2221 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2281 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2341 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2401 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     2461 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2521 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2581 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2641 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2701 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     2761 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2821 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2881 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     2941 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3001 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     3061 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     3121 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     3181 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3241 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3301 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3361 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     3421 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     3481 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     3541 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     3601 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     3661 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     3721 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     3781 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     3841 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     3901 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     3961 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     4021 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     4081 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     4141 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     4201 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     4261 ccatatggac atattgtcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat
     4321 acacatacga tttaggtgac actatagaac gcggccgcca gct
//
