LOCUS       V015835                 4257 bp    DNA     circular SYN 17-JUL-2019
DEFINITION  Exported.
ACCESSION   V015835
VERSION     V015835
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     A new toolkit for gene tagging in Candida albicans containing
            recyclable markers
  JOURNAL   PLoS ONE (2019) In press
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Instituto de Biologia Funcional y Genomica
            (IBFG), Consejo Superior de Investigaciones Cientificas, Zacarias
            Gonzalez 2, Salamanca, Salamanca 37007, Spain
REFERENCE   3  (bases 1 to 4257)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 13
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE
            (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAR-2019) Instituto de Biologia Funcional y Genomica (IBFG),
            Consejo Superior de Investigaciones Cientificas, Zacarias Gonzalez
            2, Salamanca, Salamanca 37007, Spain"
FEATURES             Location/Qualifiers
     source          1..4257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             45..71
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             75..101
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             105..131
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     regulatory      135..390
                     /note="Transcriptional termination from Saccharomyces
                     cerevisiae URA3 gene"
                     /regulatory_class="terminator"
     primer_bind     400..416
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    430..463
                     /label="loxP"
                     /note="loxP"
     CDS             811..1704
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     protein_bind    complement(1747..1780)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(1878..1896)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2154..2742)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2916..3773)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3774..3878)
                     /label="AmpR promoter"
     promoter        4224..4242
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaagcttcgt acgctgcagg tcgacgggtc gacggatccc cgggtaccca tacgatgttc
       61 ctgactatgc gggctatccc tatgacgtcc cggactatgc aggatatcca tatgacgttc
      121 cagattacgc ttagggcgcg ccattataag taaatgcatg tatactaaac tcacaaatta
      181 gagcttcaat ttaattatat cagttattac ccgggaatct cggtcgtaat gatttctata
      241 atgacgaaaa aaaaaaaatt ggaaagaaaa agcttcatgg cctttataaa aaggaactat
      301 ccaatacctc gccagaacca agtaacagta ttttacgggg cacaaatcaa gaacaataag
      361 acaggactgt aaagatggac gcatagatcc ggcggccgct ctagaactag tggatcccca
      421 tggtgatcca taacttcgta tagcatacat tatacgaagt tatagatccg gtacctggag
      481 gatgaggaga cagaagttag tagtaacaat ttggggacag aagaggatac agtgacatca
      541 gaggaagaag acagcgaagg tatagtcaaa aagcaatagg acagcaagac aatttattta
      601 tttaatagtt ttttataaat gtaaaatcta tcacaacatg tgtacttaat tttctgtggt
      661 aggggaggcc atttcgttga aacgtgtcgc gagcggttgt gagaattgac tcattttgat
      721 tattacgcaa ccattgaaat tttttatttt tttttggtga agatttttcc cacacaactt
      781 cttcttttac ttaaccacca actaccgata atggatttag tcaatcattt accagaccgt
      841 ttgttatttg ctgttcctaa aaagggcaga ttatacgaaa aatgctgtaa cttattgagt
      901 ggtgccgata tacagtttag aagatctaat agattagata tagcactttc tacaaacttg
      961 ccaattgcat taatcttctt gcctgcagct gatatcccag ttttcgttgg agaaggtaat
     1021 tgtgacttgg gtataactgg gttagaccaa atcaaagaag ctgaacaatt cgacaacatc
     1081 gaggacttgt tggatttgaa atttggttca tgcaaattgc agatccaagt tccagcagat
     1141 ggcgagtacg aaaagccaga acagcttgtt ggaaagaaaa ttgtgtcttc atttacaaaa
     1201 ttgagtaccg actatttcaa acaattgtca gacaaaccta ctaatatcag atatgtcggt
     1261 ggttccgttg aggcttcttg tgccttgggt gttgctgatg ctattgtcga tttggttgaa
     1321 agtggtgaaa ctatgaaagc agctgggtta aaggccatag aaaccatatt ggagacttcg
     1381 gctcatttga tttcgtcaaa gaaaagtaaa ttcccagaaa tggtcaatat aatcgtccaa
     1441 agacttcaag gtgttttagc tgcccaggaa tatgtcttgt gtaactacaa tgccccaaaa
     1501 tccatccaag caaaatgttt aaccattact ccaggcagaa gggccgctac tgtctctact
     1561 ttggacaaac acagcgacga cgaagaggac tgggttgcca tctcatccat ggttaataga
     1621 aaggaaatcg gtaatgtaat ggacgaattg aagaaagctg gtgcaaccga tatattggta
     1681 ctcgagatat caaattgtag agtttaaaag aagtgatagt ttctcataaa tattgctagc
     1741 ccatggataa cttcgtatag catacattat acgaagttat tagggataac agggtaatct
     1801 gcaggaaaaa cgagctcgaa ttcatcgatg atatcagatc cactagtggc ctatgcggcc
     1861 gcggatctgc cggtctccct atagtgagtc gtattaattt cgataagcca ggttaacctg
     1921 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     1981 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2041 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2101 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2161 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2221 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2281 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2341 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2401 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2461 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     2521 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     2581 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     2641 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     2701 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     2761 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     2821 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     2881 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     2941 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     3001 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     3061 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     3121 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     3181 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     3241 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     3301 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     3361 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     3421 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     3481 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     3541 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     3601 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     3661 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     3721 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     3781 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     3841 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     3901 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     3961 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     4021 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     4081 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     4141 gtactgagag tgcaccatat ggacatattg tcgttagaac gcggctacaa ttaatacata
     4201 accttatgta tcatacacat acgatttagg tgacactata gaacgcggcc gccagct
//
