LOCUS       62056_10565        4963 bp DNA     circular SYN 17-JUL-2019
DEFINITION  Cloning vector pFA-HA-LAL, complete sequence.
ACCESSION   MK652120
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F, 
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     A new toolkit for gene tagging in Candida albicans containing 
            recyclable markers
  JOURNAL   PLoS ONE (2019) In press
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F, 
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Instituto de Biologia Funcional y Genomica 
            (IBFG), Consejo Superior de Investigaciones Cientificas, Zacarias 
            Gonzalez 2, Salamanca, Salamanca 37007, Spain
REFERENCE   3  (bases 1 to 4963)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE 
            (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-MAR-2019) Instituto de Biologia Funcional y Genomica (IBFG), 
            Consejo Superior de Investigaciones Cientificas, Zacarias Gonzalez 
            2, Salamanca, Salamanca 37007, Spain"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 13
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             45..71
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             75..101
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             105..131
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     regulatory      135..390
                     /note="Transcriptional termination from Saccharomyces
                     cerevisiae URA3 gene"
                     /regulatory_class="terminator"
     primer_bind     400..416
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    418..451
                     /label=loxP
                     /note="loxP"
     gene            452..2452
                     /gene="ARG4"
                     /label=ARG4
     CDS             918..2324
                     /codon_start=1
                     /transl_table=11
                     /gene="ARG4"
                     /product="ARG4"
                     /label=ARG4
                     /note="argininosuccinate lyase; derived from Candida
                     albicans ARG4"
                     /protein_id="QDK59766.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELHLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF
                     DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG
                     ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDAIGGTAKSAVLK
                     QLENLKSILS"
     protein_bind    complement(2453..2486)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(2584..2602)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2860..3448)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3622..4479)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4480..4584)
                     /label=AmpR promoter
     promoter        4930..4948
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaagcttcgt acgctgcagg tcgacgggtc gacggatccc cgggtaccca tacgatgttc
       61 ctgactatgc gggctatccc tatgacgtcc cggactatgc aggatatcca tatgacgttc
      121 cagattacgc ttagggcgcg ccattataag taaatgcatg tatactaaac tcacaaatta
      181 gagcttcaat ttaattatat cagttattac ccgggaatct cggtcgtaat gatttctata
      241 atgacgaaaa aaaaaaaatt ggaaagaaaa agcttcatgg cctttataaa aaggaactat
      301 ccaatacctc gccagaacca agtaacagta ttttacgggg cacaaatcaa gaacaataag
      361 acaggactgt aaagatggac gcatagatcc ggcggccgct ctagaactag tggatccata
      421 acttcgtata gcatacatta tacgaagtta tagatccgta ccccccttta gtaagatttt
      481 tcaagagtag tctcaaataa acagaatgaa ggattgaagg ttttattcaa taaataattg
      541 gacagaaagt tattgtacag ttttaacaac aacaatttca aaatttcaat ttatgacata
      601 ttgaccgaca taattcagtc atttctaata ttcaaataac aagacacctt gtataaatga
      661 atccacaatc gtatatgaac aaccaaattt tatgactaac tttgggtcgt gtacttttga
      721 cgattgagtc atttttacca attaggtaat tgaattttgt taatttgtca ttaaccgcgc
      781 tggccatccc aacaataaca acaatcaatt taaaaacttg acaaggcaaa aaaaaaaaaa
      841 ttttatcttt gaaaaatttt tcttcctttt cttcttctgt tctttacata acagctatca
      901 agaataatca attaataatg tcacaacaac aagataaaca acctagtgaa aataaattat
      961 ggggtggtcg tttcaccggt gccactgatc cattgatgga tttatataat gcttcattac
     1021 cttatgataa agttatgtat gatgctgatt taactggtac taaagtatac acacaaggat
     1081 taaacaaatt aggattaatt accactgaag aattgcattt gattcatcaa ggattagaac
     1141 aaatcagaca agaatggcat gataataaat tcattattaa ggcaggtgat gaggatattc
     1201 atactgctaa tgaacgtcga ttaggagaaa tcattgggaa aaacatttct ggtaaagttc
     1261 acactggtag atcaagaaat gatcaagttg ctaccgatat gagaattttc gttcgtgaat
     1321 cattacttaa tttaagtaaa attttacatc aattcatcac ggcaattctt gaacgagcac
     1381 ataaagaaat cgatgtatta atgcctggtt atactcattt acaaaaagct caacctatta
     1441 gatgggccca ttggttaagt tcatatgcaa cttatttcac cgaagattat aaacgtttac
     1501 aagaaattat aactcgtgtt aaccaatctc cattaggatc tggtgcttta gcaggtcatc
     1561 cttatggtat tgatcgtgaa tttttagcta aaggattagg atttgatggt gtcattggga
     1621 attctttaac tgcagtttca gatcgtgatt ttgttgttga aagtttattt tggagtacat
     1681 tatttatgaa tcatatttct cgattctcag aagatctaat tatttattct agtggtgaat
     1741 ttgggttcat aaaattagct gatgcttatt ctactggttc ttcattgatg ccacaaaaga
     1801 aaaaccctga ctcattagaa ttattacgtg ggaaaagtgg tagagtattt ggtcagttat
     1861 ctggattttt aatgtcgatc aaatctattc catctactta taataaagat atgcaagagg
     1921 ataaagaacc attatttgat gcattgacta cagtggaaca ttcaatttta attgctactg
     1981 gtgtgatttc tactttactg attgataaac aaaatatgga aaaagcttta actatggata
     2041 tgttggctac tgatttagct gattatttag ttagaaaagg agttccattt agagaaactc
     2101 atcatatatc tggtgaatgt gttagaaaag ctgaagaaga aaaattatct ggaattgatc
     2161 aattatcttt tgaacaattt caacaaattg attctcgttt tgaaaaagac gttatggaaa
     2221 cttttgattt tgaagctagt gtggaaagaa gagatgctat tggtggtact gctaaaagtg
     2281 ccgttttaaa acaattagaa aatttaaaat caattttaag ttaatttata aatagtcata
     2341 taataatcac agtattgttt aacttacata aaatataatc aattaacaca gagatacctt
     2401 gtactccaat actagcattc caaccagcat ttctatacct gctagcccat ggataacttc
     2461 gtatagcata cattatacga agttattagg gataacaggg taatctgcag gaaaaacgag
     2521 ctcgaattca tcgatgatat cagatccact agtggcctat gcggccgcgg atctgccggt
     2581 ctccctatag tgagtcgtat taatttcgat aagccaggtt aacctgcatt aatgaatcgg
     2641 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     2701 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2761 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2821 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2881 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2941 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3001 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     3061 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3121 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3181 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3241 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3301 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3361 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3421 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3481 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3541 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3601 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     3661 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     3721 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     3781 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3841 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3901 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3961 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     4021 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     4081 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     4141 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     4201 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     4261 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     4321 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     4381 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     4441 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     4501 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     4561 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     4621 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     4681 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     4741 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     4801 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     4861 ccatatggac atattgtcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat
     4921 acacatacga tttaggtgac actatagaac gcggccgcca gct
//
