LOCUS       V015463                 4987 bp    DNA     circular SYN 17-JUL-2019
DEFINITION  Exported.
ACCESSION   V015463
VERSION     V015463
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     A new toolkit for gene tagging in Candida albicans containing
            recyclable markers
  JOURNAL   PLoS ONE (2019) In press
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Instituto de Biologia Funcional y Genomica
            (IBFG), Consejo Superior de Investigaciones Cientificas, Zacarias
            Gonzalez 2, Salamanca, Salamanca 37007, Spain
REFERENCE   3  (bases 1 to 4987)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 13
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE
            (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAR-2019) Instituto de Biologia Funcional y Genomica (IBFG),
            Consejo Superior de Investigaciones Cientificas, Zacarias Gonzalez
            2, Salamanca, Salamanca 37007, Spain"
FEATURES             Location/Qualifiers
     source          1..4987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             62..772
                     /label="GFP"
                     /note="green fluorescent protein"
     regulatory      776..1031
                     /note="Transcriptional termination from Saccharomyces
                     cerevisiae URA3 gene"
                     /regulatory_class="terminator"
     primer_bind     1041..1057
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    1071..1104
                     /label="loxP"
                     /note="loxP"
     CDS             1534..2343
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     protein_bind    complement(2494..2527)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(2625..2643)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2901..3489)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3663..4520)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4521..4625)
                     /label="AmpR promoter"
     promoter        4971..4987
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acgggtgctg gcgcaggtgc
       61 ttctaaaggt gaagaattat tcactggtgt tgtcccaatt ttggttgaat tagatggtga
      121 tgttaatggt cacaaatttt ctgtctccgg tgaaggtgaa ggtgatgcta cttacggtaa
      181 attgacctta aaatttattt gtactactgg taaattgcca gttccatggc caaccttagt
      241 cactacttta tgttatggtg ttcaatgttt ttcaagatac ccagatcata tgaaacaaca
      301 tgactttttc aagtctgcca tgccagaagg ttatgttcaa gaaagaacta tttttttcaa
      361 agatgacggt aactacaaga ccagagctga agtcaagttt gaaggtgata ccttagttaa
      421 tagaatcgaa ttaaaaggta ttgattttaa agaagatggt aacattttag gtcacaaatt
      481 ggaatacaac tataactctc acaatgttta catcatggct gacaaacaaa agaatggtat
      541 caaagctaac ttcaaaacta gacacaacat tgaagatggt tctgttcaat tagctgacca
      601 ttatcaacaa aatactccaa ttggtgatgg tccagtcttg ttaccagaca accattactt
      661 atccactcaa tctgccttat ccaaagatcc aaacgaaaag agagaccaca tggtcttgtt
      721 agaatttgtt actgctgctg gtattaccca tggtatggat gaattgtaca aatagggcgc
      781 gccattataa gtaaatgcat gtatactaaa ctcacaaatt agagcttcaa tttaattata
      841 tcagttatta cccgggaatc tcggtcgtaa tgatttctat aatgacgaaa aaaaaaaaat
      901 tggaaagaaa aagcttcatg gcctttataa aaaggaacta tccaatacct cgccagaacc
      961 aagtaacagt attttacggg gcacaaatca agaacaataa gacaggactg taaagatgga
     1021 cgcatagatc cggcggccgc tctagaacta gtggatccag atcttgatcc ataacttcgt
     1081 atagcataca ttatacgaag ttatagatcc agtactaata ggaattgatt tggatggtat
     1141 aaacggaaac aaaaaaaaga gctggtacta ctttctttaa aattatttta ttatttgatt
     1201 ttatttaata gtatatatta tattttgaac gtagattatt ttgttgaaag ttgctgtagt
     1261 gccattgatt cgtaacacta attctgtatt agtcattcct cttgtttgat agtatccaaa
     1321 aaaacggcta tttttttgca atcttatttc ctgcatatta tacagataac ataatgaaag
     1381 aaaaaatctt tttttttgtt cttcaatgat gatttcaacc attcttttaa acattgatca
     1441 attcctgagc aacaacccca tacacactgg tttatatatc gcccctttta cagttgaaga
     1501 aagaaataga aatagaaata gcaaacaaaa gatatgacag tcaacactaa gacctatagt
     1561 gagagagcag aaactcatgc ctcaccagta gcacaacgat tatttcgatt aatggaactg
     1621 aagaaaacca atttatgtgc atcaattgat gttgatacca ctaaggaatt ccttgaatta
     1681 attgataaat tgggtcctta tgtatgctta atcaagactc atattgatat aatcaatgat
     1741 ttttcctatg aatccactat tgaaccatta ttagaacttt cacgtaaaca tcaatttatg
     1801 atttttgaag atagaaaatt tgctgatatt ggtaataccg tgaagaaaca atatattggt
     1861 ggagtttata aaattagtag ttgggcagat attactaatg ctcatggtgt cactgggaat
     1921 ggagtagttg aaggattaaa acagggagct aaagaaacca ccaccaacca agagccaaga
     1981 gggttattga tgttagctga attatcatca gtgggatcat tagcatatgg agaatattct
     2041 caaaaaactg ttgaaattgc taaatccgat aaggaatttg ttattggatt tattgcccaa
     2101 cgtgatatgg gtggacaaga agaaggattt gattggctta ttatgacacc tggagttgga
     2161 ttagatgata aaggtgatgg attaggacaa caatatagaa ctgttgatga agttgttagc
     2221 actggaactg atattatcat tgttggtaga ggattgtttg gtaaaggaag agatccagat
     2281 attgaaggta aaaggtatag agatgctggt tggaatgctt atttgaaaaa aactggccaa
     2341 ttataaatgt gaagggggag attttcactt tattagattt gtatatatgt ataataaata
     2401 aataaataag ttaaataaat aattaaataa gggtggtaat tattactatt tacaatcaaa
     2461 ggtggtcctt ctagaagatc tgctagccca tggataactt cgtatagcat acattatacg
     2521 aagttattag ggataacagg gtaatctgca ggaaaaacga gctcgaattc atcgatgata
     2581 tcagatccac tagtggccta tgcggccgcg gatctgccgg tctccctata gtgagtcgta
     2641 ttaatttcga taagccaggt taacctgcat taatgaatcg gccaacgcgc ggggagaggc
     2701 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2761 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2821 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2881 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2941 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3001 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3061 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     3121 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3181 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3241 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3301 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     3361 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3421 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3481 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3541 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3601 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3661 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3721 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3781 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3841 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3901 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3961 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4021 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4081 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4141 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4201 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4261 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4321 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4381 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4441 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4501 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4561 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4621 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     4681 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     4741 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     4801 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     4861 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgga catattgtcg
     4921 ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga
     4981 cactata
//
