LOCUS       V016295                 4881 bp    DNA     circular SYN 17-JUL-2019
DEFINITION  Exported.
ACCESSION   V016295
VERSION     V016295
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     A new toolkit for gene tagging in Candida albicans containing
            recyclable markers
  JOURNAL   PLoS ONE (2019) In press
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Instituto de Biologia Funcional y Genomica
            (IBFG), Consejo Superior de Investigaciones Cientificas, Zacarias
            Gonzalez 2, Salamanca, Salamanca 37007, Spain
REFERENCE   3  (bases 1 to 4881)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 13
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE
            (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAR-2019) Instituto de Biologia Funcional y Genomica (IBFG),
            Consejo Superior de Investigaciones Cientificas, Zacarias Gonzalez
            2, Salamanca, Salamanca 37007, Spain"
FEATURES             Location/Qualifiers
     source          1..4881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             62..772
                     /label="GFP"
                     /note="green fluorescent protein"
     regulatory      776..1031
                     /note="Transcriptional termination from Saccharomyces
                     cerevisiae URA3 gene"
                     /regulatory_class="terminator"
     primer_bind     1041..1057
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    1071..1104
                     /label="loxP"
                     /note="loxP"
     CDS             1452..2345
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     protein_bind    complement(2388..2421)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(2519..2537)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2795..3383)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3557..4414)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4415..4519)
                     /label="AmpR promoter"
     promoter        4865..4881
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acgggtgctg gcgcaggtgc
       61 ttctaaaggt gaagaattat tcactggtgt tgtcccaatt ttggttgaat tagatggtga
      121 tgttaatggt cacaaatttt ctgtctccgg tgaaggtgaa ggtgatgcta cttacggtaa
      181 attgacctta aaatttattt gtactactgg taaattgcca gttccatggc caaccttagt
      241 cactacttta tgttatggtg ttcaatgttt ttcaagatac ccagatcata tgaaacaaca
      301 tgactttttc aagtctgcca tgccagaagg ttatgttcaa gaaagaacta tttttttcaa
      361 agatgacggt aactacaaga ccagagctga agtcaagttt gaaggtgata ccttagttaa
      421 tagaatcgaa ttaaaaggta ttgattttaa agaagatggt aacattttag gtcacaaatt
      481 ggaatacaac tataactctc acaatgttta catcatggct gacaaacaaa agaatggtat
      541 caaagctaac ttcaaaacta gacacaacat tgaagatggt tctgttcaat tagctgacca
      601 ttatcaacaa aatactccaa ttggtgatgg tccagtcttg ttaccagaca accattactt
      661 atccactcaa tctgccttat ccaaagatcc aaacgaaaag agagaccaca tggtcttgtt
      721 agaatttgtt actgctgctg gtattaccca tggtatggat gaattgtaca aatagggcgc
      781 gccattataa gtaaatgcat gtatactaaa ctcacaaatt agagcttcaa tttaattata
      841 tcagttatta cccgggaatc tcggtcgtaa tgatttctat aatgacgaaa aaaaaaaaat
      901 tggaaagaaa aagcttcatg gcctttataa aaaggaacta tccaatacct cgccagaacc
      961 aagtaacagt attttacggg gcacaaatca agaacaataa gacaggactg taaagatgga
     1021 cgcatagatc cggcggccgc tctagaacta gtggatcccc atggtgatcc ataacttcgt
     1081 atagcataca ttatacgaag ttatagatcc ggtacctgga ggatgaggag acagaagtta
     1141 gtagtaacaa tttggggaca gaagaggata cagtgacatc agaggaagaa gacagcgaag
     1201 gtatagtcaa aaagcaatag gacagcaaga caatttattt atttaatagt tttttataaa
     1261 tgtaaaatct atcacaacat gtgtacttaa ttttctgtgg taggggaggc catttcgttg
     1321 aaacgtgtcg cgagcggttg tgagaattga ctcattttga ttattacgca accattgaaa
     1381 ttttttattt ttttttggtg aagatttttc ccacacaact tcttctttta cttaaccacc
     1441 aactaccgat aatggattta gtcaatcatt taccagaccg tttgttattt gctgttccta
     1501 aaaagggcag attatacgaa aaatgctgta acttattgag tggtgccgat atacagttta
     1561 gaagatctaa tagattagat atagcacttt ctacaaactt gccaattgca ttaatcttct
     1621 tgcctgcagc tgatatccca gttttcgttg gagaaggtaa ttgtgacttg ggtataactg
     1681 ggttagacca aatcaaagaa gctgaacaat tcgacaacat cgaggacttg ttggatttga
     1741 aatttggttc atgcaaattg cagatccaag ttccagcaga tggcgagtac gaaaagccag
     1801 aacagcttgt tggaaagaaa attgtgtctt catttacaaa attgagtacc gactatttca
     1861 aacaattgtc agacaaacct actaatatca gatatgtcgg tggttccgtt gaggcttctt
     1921 gtgccttggg tgttgctgat gctattgtcg atttggttga aagtggtgaa actatgaaag
     1981 cagctgggtt aaaggccata gaaaccatat tggagacttc ggctcatttg atttcgtcaa
     2041 agaaaagtaa attcccagaa atggtcaata taatcgtcca aagacttcaa ggtgttttag
     2101 ctgcccagga atatgtcttg tgtaactaca atgccccaaa atccatccaa gcaaaatgtt
     2161 taaccattac tccaggcaga agggccgcta ctgtctctac tttggacaaa cacagcgacg
     2221 acgaagagga ctgggttgcc atctcatcca tggttaatag aaaggaaatc ggtaatgtaa
     2281 tggacgaatt gaagaaagct ggtgcaaccg atatattggt actcgagata tcaaattgta
     2341 gagtttaaaa gaagtgatag tttctcataa atattgctag cccatggata acttcgtata
     2401 gcatacatta tacgaagtta ttagggataa cagggtaatc tgcaggaaaa acgagctcga
     2461 attcatcgat gatatcagat ccactagtgg cctatgcggc cgcggatctg ccggtctccc
     2521 tatagtgagt cgtattaatt tcgataagcc aggttaacct gcattaatga atcggccaac
     2581 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     2641 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     2701 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2761 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2821 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2881 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2941 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
     3001 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3061 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3121 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3181 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     3241 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3301 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3361 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3421 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3481 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3541 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3601 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3661 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     3721 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3781 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3841 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     3901 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     3961 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4021 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4081 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4141 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4201 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4261 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4321 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4381 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4441 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4501 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     4561 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     4621 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     4681 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     4741 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     4801 tggacatatt gtcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca
     4861 tacgatttag gtgacactat a
//
