LOCUS       62056_10540        5587 bp DNA     circular SYN 17-JUL-2019
DEFINITION  Cloning vector pFA-GFP-LAL, complete sequence.
ACCESSION   MK652105
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F, 
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     A new toolkit for gene tagging in Candida albicans containing 
            recyclable markers
  JOURNAL   PLoS ONE (2019) In press
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F, 
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Instituto de Biologia Funcional y Genomica 
            (IBFG), Consejo Superior de Investigaciones Cientificas, Zacarias 
            Gonzalez 2, Salamanca, Salamanca 37007, Spain
REFERENCE   3  (bases 1 to 5587)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE 
            (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-MAR-2019) Instituto de Biologia Funcional y Genomica (IBFG), 
            Consejo Superior de Investigaciones Cientificas, Zacarias Gonzalez 
            2, Salamanca, Salamanca 37007, Spain"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 13
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             62..772
                     /label=GFP
                     /note="green fluorescent protein"
     regulatory      776..1031
                     /note="Transcriptional termination from Saccharomyces
                     cerevisiae URA3 gene"
                     /regulatory_class="terminator"
     primer_bind     1041..1057
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1059..1092
                     /label=loxP
                     /note="loxP"
     gene            1093..3093
                     /gene="ARG4"
                     /label=ARG4
     CDS             1559..2965
                     /codon_start=1
                     /transl_table=11
                     /gene="ARG4"
                     /product="ARG4"
                     /label=ARG4
                     /note="argininosuccinate lyase; derived from Candida
                     albicans ARG4"
                     /protein_id="QDK59730.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELHLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF
                     DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG
                     ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDAIGGTAKSAVLK
                     QLENLKSILS"
     protein_bind    complement(3094..3127)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(3225..3243)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3501..4089)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4263..5120)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5121..5225)
                     /label=AmpR promoter
     promoter        5571..5587
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acgggtgctg gcgcaggtgc
       61 ttctaaaggt gaagaattat tcactggtgt tgtcccaatt ttggttgaat tagatggtga
      121 tgttaatggt cacaaatttt ctgtctccgg tgaaggtgaa ggtgatgcta cttacggtaa
      181 attgacctta aaatttattt gtactactgg taaattgcca gttccatggc caaccttagt
      241 cactacttta tgttatggtg ttcaatgttt ttcaagatac ccagatcata tgaaacaaca
      301 tgactttttc aagtctgcca tgccagaagg ttatgttcaa gaaagaacta tttttttcaa
      361 agatgacggt aactacaaga ccagagctga agtcaagttt gaaggtgata ccttagttaa
      421 tagaatcgaa ttaaaaggta ttgattttaa agaagatggt aacattttag gtcacaaatt
      481 ggaatacaac tataactctc acaatgttta catcatggct gacaaacaaa agaatggtat
      541 caaagctaac ttcaaaacta gacacaacat tgaagatggt tctgttcaat tagctgacca
      601 ttatcaacaa aatactccaa ttggtgatgg tccagtcttg ttaccagaca accattactt
      661 atccactcaa tctgccttat ccaaagatcc aaacgaaaag agagaccaca tggtcttgtt
      721 agaatttgtt actgctgctg gtattaccca tggtatggat gaattgtaca aatagggcgc
      781 gccattataa gtaaatgcat gtatactaaa ctcacaaatt agagcttcaa tttaattata
      841 tcagttatta cccgggaatc tcggtcgtaa tgatttctat aatgacgaaa aaaaaaaaat
      901 tggaaagaaa aagcttcatg gcctttataa aaaggaacta tccaatacct cgccagaacc
      961 aagtaacagt attttacggg gcacaaatca agaacaataa gacaggactg taaagatgga
     1021 cgcatagatc cggcggccgc tctagaacta gtggatccat aacttcgtat agcatacatt
     1081 atacgaagtt atagatccgt accccccttt agtaagattt ttcaagagta gtctcaaata
     1141 aacagaatga aggattgaag gttttattca ataaataatt ggacagaaag ttattgtaca
     1201 gttttaacaa caacaatttc aaaatttcaa tttatgacat attgaccgac ataattcagt
     1261 catttctaat attcaaataa caagacacct tgtataaatg aatccacaat cgtatatgaa
     1321 caaccaaatt ttatgactaa ctttgggtcg tgtacttttg acgattgagt catttttacc
     1381 aattaggtaa ttgaattttg ttaatttgtc attaaccgcg ctggccatcc caacaataac
     1441 aacaatcaat ttaaaaactt gacaaggcaa aaaaaaaaaa attttatctt tgaaaaattt
     1501 ttcttccttt tcttcttctg ttctttacat aacagctatc aagaataatc aattaataat
     1561 gtcacaacaa caagataaac aacctagtga aaataaatta tggggtggtc gtttcaccgg
     1621 tgccactgat ccattgatgg atttatataa tgcttcatta ccttatgata aagttatgta
     1681 tgatgctgat ttaactggta ctaaagtata cacacaagga ttaaacaaat taggattaat
     1741 taccactgaa gaattgcatt tgattcatca aggattagaa caaatcagac aagaatggca
     1801 tgataataaa ttcattatta aggcaggtga tgaggatatt catactgcta atgaacgtcg
     1861 attaggagaa atcattggga aaaacatttc tggtaaagtt cacactggta gatcaagaaa
     1921 tgatcaagtt gctaccgata tgagaatttt cgttcgtgaa tcattactta atttaagtaa
     1981 aattttacat caattcatca cggcaattct tgaacgagca cataaagaaa tcgatgtatt
     2041 aatgcctggt tatactcatt tacaaaaagc tcaacctatt agatgggccc attggttaag
     2101 ttcatatgca acttatttca ccgaagatta taaacgttta caagaaatta taactcgtgt
     2161 taaccaatct ccattaggat ctggtgcttt agcaggtcat ccttatggta ttgatcgtga
     2221 atttttagct aaaggattag gatttgatgg tgtcattggg aattctttaa ctgcagtttc
     2281 agatcgtgat tttgttgttg aaagtttatt ttggagtaca ttatttatga atcatatttc
     2341 tcgattctca gaagatctaa ttatttattc tagtggtgaa tttgggttca taaaattagc
     2401 tgatgcttat tctactggtt cttcattgat gccacaaaag aaaaaccctg actcattaga
     2461 attattacgt gggaaaagtg gtagagtatt tggtcagtta tctggatttt taatgtcgat
     2521 caaatctatt ccatctactt ataataaaga tatgcaagag gataaagaac cattatttga
     2581 tgcattgact acagtggaac attcaatttt aattgctact ggtgtgattt ctactttact
     2641 gattgataaa caaaatatgg aaaaagcttt aactatggat atgttggcta ctgatttagc
     2701 tgattattta gttagaaaag gagttccatt tagagaaact catcatatat ctggtgaatg
     2761 tgttagaaaa gctgaagaag aaaaattatc tggaattgat caattatctt ttgaacaatt
     2821 tcaacaaatt gattctcgtt ttgaaaaaga cgttatggaa acttttgatt ttgaagctag
     2881 tgtggaaaga agagatgcta ttggtggtac tgctaaaagt gccgttttaa aacaattaga
     2941 aaatttaaaa tcaattttaa gttaatttat aaatagtcat ataataatca cagtattgtt
     3001 taacttacat aaaatataat caattaacac agagatacct tgtactccaa tactagcatt
     3061 ccaaccagca tttctatacc tgctagccca tggataactt cgtatagcat acattatacg
     3121 aagttattag ggataacagg gtaatctgca ggaaaaacga gctcgaattc atcgatgata
     3181 tcagatccac tagtggccta tgcggccgcg gatctgccgg tctccctata gtgagtcgta
     3241 ttaatttcga taagccaggt taacctgcat taatgaatcg gccaacgcgc ggggagaggc
     3301 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     3361 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3421 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3481 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3541 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3601 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3661 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     3721 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3781 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3841 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3901 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     3961 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     4021 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     4081 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     4141 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     4201 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     4261 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     4321 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     4381 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     4441 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     4501 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     4561 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4621 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4681 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4741 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4801 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4861 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4921 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4981 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     5041 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     5101 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     5161 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     5221 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     5281 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     5341 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     5401 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     5461 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgga catattgtcg
     5521 ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga
     5581 cactata
//
