LOCUS       V015792                 6421 bp    DNA     circular SYN 17-JUL-2019
DEFINITION  Exported.
ACCESSION   V015792
VERSION     V015792
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     A new toolkit for gene tagging in Candida albicans containing
            recyclable markers
  JOURNAL   PLoS ONE (2019) In press
REFERENCE   2  (bases 1 to 6421)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Instituto de Biologia Funcional y Genomica
            (IBFG), Consejo Superior de Investigaciones Cientificas, Zacarias
            Gonzalez 2, Salamanca, Salamanca 37007, Spain
REFERENCE   3  (bases 1 to 6421)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 12
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE
            (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAR-2019) Instituto de Biologia Funcional y Genomica (IBFG),
            Consejo Superior de Investigaciones Cientificas, Zacarias Gonzalez
            2, Salamanca, Salamanca 37007, Spain"
FEATURES             Location/Qualifiers
     source          1..6421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             62..772
                     /label="GFP"
                     /note="green fluorescent protein"
     regulatory      2210..2465
                     /note="Transcriptional termination from Saccharomyces
                     cerevisiae URA3 gene"
                     /regulatory_class="terminator"
     primer_bind     2475..2491
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    2505..2538
                     /product="loxP"
     CDS             2968..3777
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     protein_bind    complement(3928..3961)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(4059..4077)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4335..4923)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5097..5954)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5955..6059)
                     /label="AmpR promoter"
     promoter        6405..6421
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acgggtgctg gcgcaggtgc
       61 ttctaaaggt gaagaattat tcactggtgt tgtcccaatt ttggttgaat tagatggtga
      121 tgttaatggt cacaaatttt ctgtctccgg tgaaggtgaa ggtgatgcta cttacggtaa
      181 attgacctta aaatttattt gtactactgg taaattgcca gttccatggc caaccttagt
      241 cactacttta tgttatggtg ttcaatgttt ttcaagatac ccagatcata tgaaacaaca
      301 tgactttttc aagtctgcca tgccagaagg ttatgttcaa gaaagaacta tttttttcaa
      361 agatgacggt aactacaaga ccagagctga agtcaagttt gaaggtgata ccttagttaa
      421 tagaatcgaa ttaaaaggta ttgattttaa agaagatggt aacattttag gtcacaaatt
      481 ggaatacaac tataactctc acaatgttta catcatggct gacaaacaaa agaatggtat
      541 caaagctaac ttcaaaacta gacacaacat tgaagatggt tctgttcaat tagccgacca
      601 ttatcaacaa aatactccaa ttggtgatgg tccagtcttg ttaccagaca accattactt
      661 atccactcaa tctgccttat ccaaagatcc aaacgaaaag agagaccaca tggtcttgtt
      721 agaatttgtt actgctgctg gtattaccca tggtatggat gaattgtaca aaagatcctc
      781 taaaggtgaa gaattattca ctggtgttgt cccaattttg gttgaattag atggtgatgt
      841 taatggtcac aaattttctg tctccggtga aggtgaaggt gatgctactt acggtaaatt
      901 gaccttaaaa tttatttgta ctactggtaa attgccagtt ccatggccaa ccttagtcac
      961 tactttatgt tatggtgttc aatgtttttc aagataccca gatcatatga aacaacatga
     1021 ctttttcaag tctgccatgc cagaaggtta tgttcaagaa agaactattt ttttcaaaga
     1081 tgacggtaac tacaagacca gagctgaagt caagtttgaa ggtgatacct tagttaatag
     1141 aatcgaatta aaaggtattg attttaaaga agatggtaac attttaggtc acaaattgga
     1201 atacaactat aactctcaca atgtttacat catggctgac aaacaaaaga atggtatcaa
     1261 agctaacttc aaaactagac acaacattga agatggttct gttcaattag ctgaccatta
     1321 tcaacaaaat actccaattg gtgatggtcc agtcttgtta ccagacaacc attacttatc
     1381 cactcaatct gccttatcca aagatccaaa cgaaaagaga gaccacatgg tcttgttaga
     1441 atttgttact gctgctggta ttacccatgg tatggatgaa ttgtacaaaa gatcctctaa
     1501 aggtgaagaa ttattcactg gtgttgtccc aattttggtt gaattagatg gtgatgttaa
     1561 tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat gctacttacg gtaaattgac
     1621 cttaaaattt atttgtacta ctggtaaatt gccagttcca tggccaacct tagtcactac
     1681 tttatgttat ggtgttcaat gtttttcaag atacccagat catatgaaac aacatgactt
     1741 tttcaagtct gccatgccag aaggttatgt tcaagaaaga actatttttt tcaaagatga
     1801 cggtaactac aagaccagag ctgaagtcaa gtttgaaggt gataccttag ttaatagaat
     1861 cgaattaaaa ggtattgatt ttaaagaaga tggtaacatt ttaggtcaca aattggaata
     1921 caactataac tctcacaatg tttacatcat ggctgacaaa caaaagaatg gtatcaaagc
     1981 taacttcaaa actagacaca acattgaaga tggttctgtt caattagctg accattatca
     2041 acaaaatact ccaattggtg atggtccagt cttgttacca gacaaccatt acttatccac
     2101 tcaatctgcc ttatccaaag atccaaacga aaagagagac cacatggtct tgttagaatt
     2161 tgttactgct gctggtatta cccatggtat ggatgaattg tacaaataag gcgcgccatt
     2221 ataagtaaat gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt
     2281 attacccggg aatctcggtc gtaatgattt ctataatgac gaaaaaaaaa aaattggaaa
     2341 gaaaaagctt catggccttt ataaaaagga actatccaat acctcgccag aaccaagtaa
     2401 cagtatttta cggggcacaa atcaagaaca ataagacagg actgtaaaga tggacgcata
     2461 gatccggcgg ccgctctaga actagtggat ccagatcttg atccataact tcgtatagca
     2521 tacattatac gaagttatag atccagtact aataggaatt gatttggatg gtataaacgg
     2581 aaacaaaaaa aagagctggt actactttct ttaaaattat tttattattt gattttattt
     2641 aatagtatat attatatttt gaacgtagat tattttgttg aaagttgctg tagtgccatt
     2701 gattcgtaac actaattctg tattagtcat tcctcttgtt tgatagtatc caaaaaaacg
     2761 gctatttttt tgcaatctta tttcctgcat attatacaga taacataatg aaagaaaaaa
     2821 tctttttttt tgttcttcaa tgatgatttc aaccattctt ttaaacattg atcaattcct
     2881 gagcaacaac cccatacaca ctggtttata tatcgcccct tttacagttg aagaaagaaa
     2941 tagaaataga aatagcaaac aaaagatatg acagtcaaca ctaagaccta tagtgagaga
     3001 gcagaaactc atgcctcacc agtagcacaa cgattatttc gattaatgga actgaagaaa
     3061 accaatttat gtgcatcaat tgatgttgat accactaagg aattccttga attaattgat
     3121 aaattgggtc cttatgtatg cttaatcaag actcatattg atataatcaa tgatttttcc
     3181 tatgaatcca ctattgaacc attattagaa ctttcacgta aacatcaatt tatgattttt
     3241 gaagatagaa aatttgctga tattggtaat accgtgaaga aacaatatat tggtggagtt
     3301 tataaaatta gtagttgggc agatattact aatgctcatg gtgtcactgg gaatggagta
     3361 gttgaaggat taaaacaggg agctaaagaa accaccacca accaagagcc aagagggtta
     3421 ttgatgttag ctgaattatc atcagtggga tcattagcat atggagaata ttctcaaaaa
     3481 actgttgaaa ttgctaaatc cgataaggaa tttgttattg gatttattgc ccaacgtgat
     3541 atgggtggac aagaagaagg atttgattgg cttattatga cacctggagt tggattagat
     3601 gataaaggtg atggattagg acaacaatat agaactgttg atgaagttgt tagcactgga
     3661 actgatatta tcattgttgg tagaggattg tttggtaaag gaagagatcc agatattgaa
     3721 ggtaaaaggt atagagatgc tggttggaat gcttatttga aaaaaactgg ccaattataa
     3781 atgtgaaggg ggagattttc actttattag atttgtatat atgtataata aataaataaa
     3841 taagttaaat aaataattaa ataagggtgg taattattac tatttacaat caaaggtggt
     3901 ccttctagaa gatctgctag cccatggata acttcgtata gcatacatta tacgaagtta
     3961 ttagggataa cagggtaatc tgcaggaaaa acgagctcga attcatcgat gatatcagat
     4021 ccactagtgg cctatgcggc cgcggatctg ccggtctccc tatagtgagt cgtattaatt
     4081 tcgataagcc aggttaacct gcattaatga atcggccaac gcgcggggag aggcggtttg
     4141 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     4201 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4261 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4321 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4381 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4441 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4501 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     4561 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4621 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4681 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4741 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     4801 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4861 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4921 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4981 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5041 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5101 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5161 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5221 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5281 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5341 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5401 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     5461 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5521 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5581 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5641 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5701 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5761 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5821 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     5881 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     5941 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6001 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6061 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     6121 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     6181 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     6241 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     6301 tatgcggcat cagagcagat tgtactgaga gtgcaccata tggacatatt gtcgttagaa
     6361 cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat
     6421 a
//
