LOCUS       V015277                 6315 bp    DNA     circular SYN 17-JUL-2019
DEFINITION  Exported.
ACCESSION   V015277
VERSION     V015277
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     A new toolkit for gene tagging in Candida albicans containing
            recyclable markers
  JOURNAL   PLoS ONE (2019) In press
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Instituto de Biologia Funcional y Genomica
            (IBFG), Consejo Superior de Investigaciones Cientificas, Zacarias
            Gonzalez 2, Salamanca, Salamanca 37007, Spain
REFERENCE   3  (bases 1 to 6315)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 12
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE
            (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAR-2019) Instituto de Biologia Funcional y Genomica (IBFG),
            Consejo Superior de Investigaciones Cientificas, Zacarias Gonzalez
            2, Salamanca, Salamanca 37007, Spain"
FEATURES             Location/Qualifiers
     source          1..6315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             62..772
                     /label="GFP"
                     /note="green fluorescent protein"
     regulatory      2210..2465
                     /note="Transcriptional termination from Saccharomyces
                     cerevisiae URA3 gene"
                     /regulatory_class="terminator"
     primer_bind     2475..2491
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    2505..2538
                     /product="loxP"
     CDS             2886..3779
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     protein_bind    complement(3822..3855)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(3953..3971)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4229..4817)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4991..5848)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5849..5953)
                     /label="AmpR promoter"
     promoter        6299..6315
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acgggtgctg gcgcaggtgc
       61 ttctaaaggt gaagaattat tcactggtgt tgtcccaatt ttggttgaat tagatggtga
      121 tgttaatggt cacaaatttt ctgtctccgg tgaaggtgaa ggtgatgcta cttacggtaa
      181 attgacctta aaatttattt gtactactgg taaattgcca gttccatggc caaccttagt
      241 cactacttta tgttatggtg ttcaatgttt ttcaagatac ccagatcata tgaaacaaca
      301 tgactttttc aagtctgcca tgccagaagg ttatgttcaa gaaagaacta tttttttcaa
      361 agatgacggt aactacaaga ccagagctga agtcaagttt gaaggtgata ccttagttaa
      421 tagaatcgaa ttaaaaggta ttgattttaa agaagatggt aacattttag gtcacaaatt
      481 ggaatacaac tataactctc acaatgttta catcatggct gacaaacaaa agaatggtat
      541 caaagctaac ttcaaaacta gacacaacat tgaagatggt tctgttcaat tagccgacca
      601 ttatcaacaa aatactccaa ttggtgatgg tccagtcttg ttaccagaca accattactt
      661 atccactcaa tctgccttat ccaaagatcc aaacgaaaag agagaccaca tggtcttgtt
      721 agaatttgtt actgctgctg gtattaccca tggtatggat gaattgtaca aaagatcctc
      781 taaaggtgaa gaattattca ctggtgttgt cccaattttg gttgaattag atggtgatgt
      841 taatggtcac aaattttctg tctccggtga aggtgaaggt gatgctactt acggtaaatt
      901 gaccttaaaa tttatttgta ctactggtaa attgccagtt ccatggccaa ccttagtcac
      961 tactttatgt tatggtgttc aatgtttttc aagataccca gatcatatga aacaacatga
     1021 ctttttcaag tctgccatgc cagaaggtta tgttcaagaa agaactattt ttttcaaaga
     1081 tgacggtaac tacaagacca gagctgaagt caagtttgaa ggtgatacct tagttaatag
     1141 aatcgaatta aaaggtattg attttaaaga agatggtaac attttaggtc acaaattgga
     1201 atacaactat aactctcaca atgtttacat catggctgac aaacaaaaga atggtatcaa
     1261 agctaacttc aaaactagac acaacattga agatggttct gttcaattag ctgaccatta
     1321 tcaacaaaat actccaattg gtgatggtcc agtcttgtta ccagacaacc attacttatc
     1381 cactcaatct gccttatcca aagatccaaa cgaaaagaga gaccacatgg tcttgttaga
     1441 atttgttact gctgctggta ttacccatgg tatggatgaa ttgtacaaaa gatcctctaa
     1501 aggtgaagaa ttattcactg gtgttgtccc aattttggtt gaattagatg gtgatgttaa
     1561 tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat gctacttacg gtaaattgac
     1621 cttaaaattt atttgtacta ctggtaaatt gccagttcca tggccaacct tagtcactac
     1681 tttatgttat ggtgttcaat gtttttcaag atacccagat catatgaaac aacatgactt
     1741 tttcaagtct gccatgccag aaggttatgt tcaagaaaga actatttttt tcaaagatga
     1801 cggtaactac aagaccagag ctgaagtcaa gtttgaaggt gataccttag ttaatagaat
     1861 cgaattaaaa ggtattgatt ttaaagaaga tggtaacatt ttaggtcaca aattggaata
     1921 caactataac tctcacaatg tttacatcat ggctgacaaa caaaagaatg gtatcaaagc
     1981 taacttcaaa actagacaca acattgaaga tggttctgtt caattagctg accattatca
     2041 acaaaatact ccaattggtg atggtccagt cttgttacca gacaaccatt acttatccac
     2101 tcaatctgcc ttatccaaag atccaaacga aaagagagac cacatggtct tgttagaatt
     2161 tgttactgct gctggtatta cccatggtat ggatgaattg tacaaataag gcgcgccatt
     2221 ataagtaaat gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt
     2281 attacccggg aatctcggtc gtaatgattt ctataatgac gaaaaaaaaa aaattggaaa
     2341 gaaaaagctt catggccttt ataaaaagga actatccaat acctcgccag aaccaagtaa
     2401 cagtatttta cggggcacaa atcaagaaca ataagacagg actgtaaaga tggacgcata
     2461 gatccggcgg ccgctctaga actagtggat ccccatggtg atccataact tcgtatagca
     2521 tacattatac gaagttatag atccggtacc tggaggatga ggagacagaa gttagtagta
     2581 acaatttggg gacagaagag gatacagtga catcagagga agaagacagc gaaggtatag
     2641 tcaaaaagca ataggacagc aagacaattt atttatttaa tagtttttta taaatgtaaa
     2701 atctatcaca acatgtgtac ttaattttct gtggtagggg aggccatttc gttgaaacgt
     2761 gtcgcgagcg gttgtgagaa ttgactcatt ttgattatta cgcaaccatt gaaatttttt
     2821 attttttttt ggtgaagatt tttcccacac aacttcttct tttacttaac caccaactac
     2881 cgataatgga tttagtcaat catttaccag accgtttgtt atttgctgtt cctaaaaagg
     2941 gcagattata cgaaaaatgc tgtaacttat tgagtggtgc cgatatacag tttagaagat
     3001 ctaatagatt agatatagca ctttctacaa acttgccaat tgcattaatc ttcttgcctg
     3061 cagctgatat cccagttttc gttggagaag gtaattgtga cttgggtata actgggttag
     3121 accaaatcaa agaagctgaa caattcgaca acatcgagga cttgttggat ttgaaatttg
     3181 gttcatgcaa attgcagatc caagttccag cagatggcga gtacgaaaag ccagaacagc
     3241 ttgttggaaa gaaaattgtg tcttcattta caaaattgag taccgactat ttcaaacaat
     3301 tgtcagacaa acctactaat atcagatatg tcggtggttc cgttgaggct tcttgtgcct
     3361 tgggtgttgc tgatgctatt gtcgatttgg ttgaaagtgg tgaaactatg aaagcagctg
     3421 ggttaaaggc catagaaacc atattggaga cttcggctca tttgatttcg tcaaagaaaa
     3481 gtaaattccc agaaatggtc aatataatcg tccaaagact tcaaggtgtt ttagctgccc
     3541 aggaatatgt cttgtgtaac tacaatgccc caaaatccat ccaagcaaaa tgtttaacca
     3601 ttactccagg cagaagggcc gctactgtct ctactttgga caaacacagc gacgacgaag
     3661 aggactgggt tgccatctca tccatggtta atagaaagga aatcggtaat gtaatggacg
     3721 aattgaagaa agctggtgca accgatatat tggtactcga gatatcaaat tgtagagttt
     3781 aaaagaagtg atagtttctc ataaatattg ctagcccatg gataacttcg tatagcatac
     3841 attatacgaa gttattaggg ataacagggt aatctgcagg aaaaacgagc tcgaattcat
     3901 cgatgatatc agatccacta gtggcctatg cggccgcgga tctgccggtc tccctatagt
     3961 gagtcgtatt aatttcgata agccaggtta acctgcatta atgaatcggc caacgcgcgg
     4021 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     4081 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     4141 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     4201 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     4261 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     4321 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     4381 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
     4441 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     4501 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4561 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     4621 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     4681 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4741 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4801 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4861 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4921 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     4981 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     5041 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     5101 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     5161 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     5221 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     5281 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     5341 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     5401 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     5461 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     5521 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     5581 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     5641 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5701 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5761 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5821 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5881 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5941 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     6001 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     6061 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     6121 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     6181 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatggaca
     6241 tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat
     6301 ttaggtgaca ctata
//
