LOCUS       62056_10510        4889 bp DNA     circular SYN 30-SEP-2020
DEFINITION  Cloning vector pFA-3xFLAG-MNase-SAT1, complete sequence.
ACCESSION   MT223485
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Tebbji F, Khemiri I, Sellam A.
  TITLE     High resolution genome-wide occupancy in Candida spp. using ChEC-seq
  JOURNAL   bioRxiv (2020) In press
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Sellam A, Tebbji F, Khemiri I.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 4889)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-MAR-2020) Microbiology, Infectious Disease "
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             63..128
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags, followed by an 
                     enterokinase cleavage site"
     CDS             135..581
                     /label=MNase
                     /note="micrococcal nuclease from Staphylococcus aureus"
     CDS             join(1101..1110,1765..2327)
                     /codon_start=1
                     /transl_table=11
                     /product="Sat1"
                     /label=Sat1
                     /note="streptomycin acetyltransferase"
                     /protein_id="QOE54956.1"
                     /translation="MDGEEVAALVIDNGSHMKISVIPEQVAETLDAENHFIVREVFDVH
                     LSDQGFELSTRSVSPYRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEH
                     IVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDL
                     FTYKTRPQVSNETAMYWYWFSGAQDDA"
     promoter        complement(2527..2545)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2803..3391)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3565..4422)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4423..4527)
                     /label=AmpR promoter
     promoter        4873..4889
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 aagattataa ggaccatgac ggagattaca aagatcacga cattgactac aaagatgacg
      121 acgataaagc tgccgccaca tctaccaaga aacttcataa ggaaccagct acattgatta
      181 aggcaataga tggggacacc gtaaaactta tgtacaaagg gcagccaatg accttcagat
      241 tgttgttggt tgacactcct gaaacaaaac acccaaagaa aggagtggaa aaatatggtc
      301 ctgaggcttc cgctttcaca aagaaaatgg tagaaaacgc aaagaagatc gaagtcgagt
      361 tcgacaaagg tcaacgtacc gataaatatg gaagaggact tgcatacatt tacgccgatg
      421 gaaaaatggt gaatgaagca ttggtgagac aagggttagc aaaagttgca tatgtctaca
      481 agccaaataa cacacacgag cagcatttac gtaaatcaga ggcacaggca aagaaagaga
      541 aattaaacat ttggagtgag gataatgctg attcagggca atagtagagg cgcgccgtcg
      601 agcgtcaaaa ctagagaata ataaagaaaa cgatcttttc aaaaagaaaa aaccttttag
      661 ttttcctttg ttgttgttgt gggtgtgtgc tatttatatt atatagttta ctcataatac
      721 cataaaatat tcggtttgat taggttattt taataagcta atttgtttct aatcgtgtaa
      781 tttatgctgt gtatattaag tagtgtgtgc actgcccaaa aatgtttgtt gtttatagtc
      841 ggttaaagag aaaaaagaaa aaaagatcca tacacacacg ttaattagtt gttcaacgta
      901 atacactcat attttgttct tatttgcttt cggtcgctgt tctcaccaag atttattgcc
      961 aacgaaacaa ttttttttta tatattttca gatttttctt tttttccttt cctttccttt
     1021 tctaattttc actcctggtt ttctttcttt cttagaaaca ttatctcgat attaatatta
     1081 aaaaaatata atcattcaaa atggacggtg gtatgtttta gtttagcttc aattctaatt
     1141 gattgattaa tcagttgatt ggtttcaata tgacaaatgg gtagggtggg aaaacttcat
     1201 tttcaattca gatcaaactt ttttgttgtc gacataatat ttctcgtttg ggatgttact
     1261 gtcacattaa taatacacac acatcagctt ataattttga aagtaattta tcagatatgt
     1321 tgtgacgatc aatggaaatg gctaacttca atgtatctgt tcttcccctt tttcaaagtt
     1381 cacgtttttt gattgattga ttgatctgtc ggcagtggtt tcaaaaccat tcggtgagta
     1441 atcctatcaa tcaatgttac gacaaaaggc tcaatattca aaattgcaat gttttatgtt
     1501 ttcctacgtg tacttgtgca aggcaattga ttcaacattg cttttggtgt ttgacgagtt
     1561 tctagtttgg acttgtgttg ttatctgggc tatacagatt tcccggctca ctatgaattt
     1621 tttttttcga cgctcagtgc acacaactat aaacaacaca aacacaaaca cagcaagaaa
     1681 aaaaaaaaac gaacattgaa ttgaaaccaa gccaactgaa aaattcctta tttaaatgac
     1741 tgtcatacta acccattttt atagaagaag ttgctgcttt agttatcgat aacggttctc
     1801 atatgaaaat ttcggtgatc cctgagcagg tggcggaaac attggatgct gagaaccatt
     1861 tcattgttcg tgaagtgttc gatgtgcacc tatccgacca aggctttgaa ctatctacca
     1921 gaagtgtgag cccctaccgg aaggattaca tctcggatga tgactctgat gaagactctg
     1981 cttgctatgg cgcattcatc gaccaagagc ttgtcgggaa gattgaactc aactcaacat
     2041 ggaacgatct agcctctatc gaacacattg ttgtgtcgca cacgcaccga ggcaaaggag
     2101 tcgcgcacag tctcatcgaa tttgcgaaaa agtgggcact aagcagacag ctccttggca
     2161 tacgattaga gacacaaacg aacaatgtac ctgcctgcaa tttgtacgca aaatgtggct
     2221 ttactctcgg cggcattgac ctcttcacgt ataaaactag acctcaagtc tcgaacgaaa
     2281 cagcgatgta ctggtactgg ttctcgggag cacaggatga cgcctaacat atgtgaagtg
     2341 tgaaggggga gattttcact ttattagatt tgtatatatg tataataaat aaataaataa
     2401 gttaaataaa taattagata agggtggtaa ttattactat ttacaatcaa aggtggtcct
     2461 gagctcgaat tcatcgatga tatcagatcc actagtggcc tatgcggccg cggatctgcc
     2521 ggtctcccta tagtgagtcg tattaatttc gataagccag gttaacctgc attaatgaat
     2581 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     2641 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2701 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2761 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2821 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2881 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2941 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
     3001 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3061 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3121 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3181 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3241 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3301 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3361 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3421 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3481 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3541 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3601 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3661 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     3721 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3781 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3841 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3901 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3961 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     4021 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     4081 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     4141 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     4201 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     4261 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     4321 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     4381 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     4441 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     4501 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     4561 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     4621 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     4681 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     4741 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     4801 gcaccatatg gacatattgt cgttagaacg cggctacaat taatacataa ccttatgtat
     4861 catacacata cgatttaggt gacactata
//
