LOCUS       V015645                 4623 bp    DNA     circular SYN 30-SEP-2020
DEFINITION  Exported.
ACCESSION   V015645
VERSION     V015645
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Tebbji F, Khemiri I, Sellam A.
  TITLE     High resolution genome-wide occupancy in Candida spp. using ChEC-seq
  JOURNAL   bioRxiv (2020) In press
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Sellam A, Tebbji F, Khemiri I.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4623)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv
            (2020) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-MAR-2020) Microbiology, Infectious Disease "
FEATURES             Location/Qualifiers
     source          1..4623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             63..128
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
     CDS             135..581
                     /label="MNase"
                     /note="micrococcal nuclease from Staphylococcus aureus"
     CDS             1247..2056
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     promoter        complement(2261..2279)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2537..3125)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3299..4156)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4157..4261)
                     /label="AmpR promoter"
     promoter        4607..4623
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 aagattataa ggaccatgac ggagattaca aagatcacga cattgactac aaagatgacg
      121 acgataaagc tgccgccaca tctaccaaga aacttcataa ggaaccagct acattgatta
      181 aggcaataga tggggacacc gtaaaactta tgtacaaagg gcagccaatg accttcagat
      241 tgttgttggt tgacactcct gaaacaaaac acccaaagaa aggagtggaa aaatatggtc
      301 ctgaggcttc cgctttcaca aagaaaatgg tagaaaacgc aaagaagatc gaagtcgagt
      361 tcgacaaagg tcaacgtacc gataaatatg gaagaggact tgcatacatt tacgccgatg
      421 gaaaaatggt gaatgaagca ttggtgagac aagggttagc aaaagttgca tatgtctaca
      481 agccaaataa cacacacgag cagcatttac gtaaatcaga ggcacaggca aagaaagaga
      541 aattaaacat ttggagtgag gataatgctg attcagggca atagtagagg cgcgccatta
      601 taagtaaatg catgtatact aaactcacaa attagagctt caatttaatt atatcagtta
      661 ttacccggga atctcggtcg taatgatttc tataatgacg aaaaaaaaaa aattggaaag
      721 aaaaagcttc atggccttta taaaaaggaa ctatccaata cctcgccaga accaagtaac
      781 agtattttac ggggcacaaa tcaagaacaa taagacagga ctgtaaagat ggacgcatag
      841 atccggatgg tataaacgga aacaaaaaaa agagctggta ctactttctt taaaattatt
      901 ttattatttg attttattta atagtatata ttatattttg aacgtagatt attttgttga
      961 aagttgctgt agtgccattg attcgtaaca ctaattctgt attagtcatt cctcttgttt
     1021 gatagtatcc aaaaaaacgg ctattttttt gcaatcttat ttcctgcata ttatacagat
     1081 aacataatga aagaaaaaat cttttttttt gttcttcaat gatgatttca accattcttt
     1141 taaacattga tcaattcctg agcaacaacc ccatacacac tggtttatat accgcccctt
     1201 ttacagttga agaaagaaat agaaatagaa atagcaaaca aaagatatga cagtcaacac
     1261 taagacctat agtgagagag cagaaactca tgcctcacca gtagcacagc gattatttcg
     1321 attaatggaa ctgaagaaaa ccaatttatg tgcatcaatt gacgttgata ccactaagga
     1381 attccttgaa ttaattgata aattaggtcc ttatgtatgc ttaatcaaga ctcatattga
     1441 tataatcaat gatttttcct atgaatccac tattgaacca ttattagaac tttcacgtaa
     1501 acatcaattt atgatttttg aagatagaaa atttgctgat attggtaata ccgtaaagaa
     1561 acaatatatt ggtggagttt ataaaattag tagttgggca gatattacca atgctcatgg
     1621 tgtcactggg aatggagtgg ttgaaggatt aaaacaggga gctaaagaaa ccaccaccaa
     1681 ccaagagcca agagggttat tgatgttagc tgaattatca tcagtgggat cattagcata
     1741 tggagaatat tctcaaaaaa ctgttgaaat tgctaaatcc gataaggaat ttgttattgg
     1801 atttattgcc caacgtgata tgggtggcca agaagaagga tttgattggc ttattatgac
     1861 acctggagtt ggattagatg ataaaggtga tggattagga caacaatata gaactgttga
     1921 tgaagttgtt agcactggaa ctgatattat cattgttggt agaggattgt ttggtaaagg
     1981 aagagatcca gatattgaag gtaaaaggta tagaaatgct ggttggaatg cttatttgaa
     2041 aaagactggc caattataaa tgtgaagggg gagattttca ctttattaga tttgtatata
     2101 tgtagaataa ataaataaat aagttaaata aataattaaa taagggtggt aattattact
     2161 atttacaatc aaaggtggtc cttctagttt aaacgagctc gaattcatcg atgatatcag
     2221 atccactagt ggcctatgcg gccgcggatc tgccggtctc cctatagtga gtcgtattaa
     2281 tttcgataag ccaggttaac ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2341 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     2401 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     2461 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2521 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2581 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2641 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2701 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     2761 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2821 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2881 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2941 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     3001 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3061 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3121 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3181 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3241 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3301 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3361 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3421 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3481 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3541 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     3601 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3661 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3721 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3781 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3841 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3901 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3961 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4021 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4081 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4141 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4201 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     4261 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     4321 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     4381 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     4441 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     4501 actatgcggc atcagagcag attgtactga gagtgcacca tatggacata ttgtcgttag
     4561 aacgcggcta caattaatac ataaccttat gtatcataca catacgattt aggtgacact
     4621 ata
//
