LOCUS       V015324                 4644 bp    DNA     circular SYN 30-SEP-2020
DEFINITION  Exported.
ACCESSION   V015324
VERSION     V015324
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Tebbji F, Khemiri I, Sellam A.
  TITLE     High resolution genome-wide occupancy in Candida spp. using ChEC-seq
  JOURNAL   bioRxiv (2020) In press
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Sellam S, Tebbji F, Khemiri I.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4644)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv
            (2020) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-MAR-2020) Microbiology, Infectious Disease "
FEATURES             Location/Qualifiers
     source          1..4644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             63..128
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
     CDS             135..581
                     /label="MNase"
                     /note="micrococcal nuclease from Staphylococcus aureus"
     CDS             1180..2073
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     promoter        complement(2282..2300)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2558..3146)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3320..4177)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4178..4282)
                     /label="AmpR promoter"
     promoter        4628..4644
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 aagattataa ggaccatgac ggagattaca aagatcacga cattgactac aaagatgacg
      121 acgataaagc tgccgccaca tctaccaaga aacttcataa ggaaccagct acattgatta
      181 aggcaataga tggggacacc gtaaaactta tgtacaaagg gcagccaatg accttcagat
      241 tgttgttggt tgacactcct gaaacaaaac acccaaagaa aggagtggaa aaatatggtc
      301 ctgaggcttc cgctttcaca aagaaaatgg tagaaaacgc aaagaagatc gaagtcgagt
      361 tcgacaaagg tcaacgtacc gataaatatg gaagaggact tgcatacatt tacgccgatg
      421 gaaaaatggt gaatgaagca ttggtgagac aagggttagc aaaagttgca tatgtctaca
      481 agccaaataa cacacacgag cagcatttac gtaaatcaga ggcacaggca aagaaagaga
      541 aattaaacat ttggagtgag gataatgctg attcagggca atagtagagg cgcgccatta
      601 taagtaaatg catgtatact aaactcacaa attagagctt caatttaatt atatcagtta
      661 ttacccggga atctcggtcg taatgatttc tataatgacg aaaaaaaaaa aattggaaag
      721 aaaaagcttc atggccttta taaaaaggaa ctatccaata cctcgccaga accaagtaac
      781 agtattttac ggggcacaaa tcaagaacaa taagacagga ctgtaaagat ggacgcatag
      841 atcctggagg atgaggagac agaagttagt agtaacaatt tggggacaga agaggataca
      901 gtgacatcag aggaagaaga cagcgaaggt atagtcaaaa agcaatagga cagcaagaca
      961 atttatttat ttaatagttt tttataaatg taaaatctat cacaacatgt gtacttaatt
     1021 ttctgtggta ggggaggcca tttcgttgaa acgtgtcgcg agcggttgtg agaattgact
     1081 cattttgatt attacgcaac cattgaaatt ttttattttt ttttggtgaa gatttttccc
     1141 acacaacttc ttcttttact taaccaccaa ctaccgataa tggatttagt caatcattta
     1201 ccagaccgtt tgttatttgc tgttcctaaa aagggcagat tatacgaaaa atgctgtaac
     1261 ttattgagtg gtgccgatat acagtttaga agatctaata gattagatat agcactttct
     1321 acaaacttgc caattgcatt aatcttcttg cctgcagctg atatcccagt tttcgttgga
     1381 gaaggtaatt gtgacttggg tataactggg ttagaccaaa tcaaagaagc tgaacaattc
     1441 gacaacatcg aggacttgtt ggatttgaaa tttggttcat gcaaattgca gatccaagtt
     1501 ccagcagatg gcgagtacga aaagccagaa cagcttgttg gaaagaaaat tgtgtcttca
     1561 tttacaaaat tgagtaccga ctatttcaaa caattgtcag acaaacctac taatatcaga
     1621 tatgtcggtg gttccgttga ggcttcttgt gccttgggtg ttgctgatgc tattgtcgat
     1681 ttggttgaaa gtggtgaaac tatgaaagca gctgggttaa aggccataga aaccatattg
     1741 gagacttcgg ctcatttgat ttcgtcaaag aaaagtaaat tcccagaaat ggtcaatata
     1801 atcgtccaaa gacttcaagg tgttttagct gcccaggaat atgtcttgtg taactacaat
     1861 gccccaaaat ccatccaagc aaaatgttta accattactc caggcagaag ggccgctact
     1921 gtctctactt tggacaaaca cagcgacgac gaagaggact gggttgccat ctcatccatg
     1981 gttaatagaa aggaaatcgg taatgtaatg gacgaattga agaaagctgg tgcaaccgat
     2041 atattggtac tcgagatatc aaattgtaga gtttaaaaga agtgatagtt tctcataaat
     2101 attatacata tatacttata gatctacttc aatttgactc aatctgtctt ataccatctt
     2161 tatctaccac cttgatgtac acacctttaa agtcaatagg cattctcgtt taaacgagct
     2221 cgaattcatc gatgatatca gatccactag tggcctatgc ggccgcggat ctgccggtct
     2281 ccctatagtg agtcgtatta atttcgataa gccaggttaa cctgcattaa tgaatcggcc
     2341 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     2401 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     2461 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2521 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2581 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2641 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2701 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     2761 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2821 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2881 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2941 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     3001 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3061 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3121 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3181 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     3241 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3301 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3361 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     3421 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3481 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3541 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3601 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3661 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3721 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3781 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3841 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3901 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3961 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4021 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4081 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4141 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4201 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4261 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     4321 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     4381 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     4441 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     4501 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     4561 atatggacat attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac
     4621 acatacgatt taggtgacac tata
//
