LOCUS       62056_10495        5232 bp DNA     circular SYN 30-SEP-2020
DEFINITION  Cloning vector pFA-3xFLAG-MNase-CaARG4, complete sequence.
ACCESSION   MT181238
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Tebbji F, Khemiri I, Sellam A.
  TITLE     High resolution genome-wide occupancy in Candida spp. using ChEC-seq
  JOURNAL   bioRxiv (2020) In press
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Sellam A, Tebbji F, Khemiri I.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 5232)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-MAR-2020) Microbiology, Infectious Disease "
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             63..128
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags, followed by an 
                     enterokinase cleavage site"
     CDS             135..581
                     /label=MNase
                     /note="micrococcal nuclease from Staphylococcus aureus"
     CDS             1302..2708
                     /codon_start=1
                     /transl_table=11
                     /product="CaArg4"
                     /label=CaArg4
                     /note="CaArg4; argininosuccinate lyase"
                     /protein_id="QOE54958.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELNLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF
                     DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG
                     ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDALGGTAKSAVLK
                     QLENLKSILS"
     promoter        complement(2870..2888)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3146..3734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3908..4765)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4766..4870)
                     /label=AmpR promoter
     promoter        5216..5232
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 aagattataa ggaccatgac ggagattaca aagatcacga cattgactac aaagatgacg
      121 acgataaagc tgccgccaca tctaccaaga aacttcataa ggaaccagct acattgatta
      181 aggcaataga tggggacacc gtaaaactta tgtacaaagg gcagccaatg accttcagat
      241 tgttgttggt tgacactcct gaaacaaaac acccaaagaa aggagtggaa aaatatggtc
      301 ctgaggcttc cgctttcaca aagaaaatgg tagaaaacgc aaagaagatc gaagtcgagt
      361 tcgacaaagg tcaacgtacc gataaatatg gaagaggact tgcatacatt tacgccgatg
      421 gaaaaatggt gaatgaagca ttggtgagac aagggttagc aaaagttgca tatgtctaca
      481 agccaaataa cacacacgag cagcatttac gtaaatcaga ggcacaggca aagaaagaga
      541 aattaaacat ttggagtgag gataatgctg attcagggca atagtagagg cgcgccatta
      601 taagtaaatg catgtatact aaactcacaa attagagctt caatttaatt atatcagtta
      661 ttacccggga atctcggtcg taatgatttc tataatgacg aaaaaaaaaa aattggaaag
      721 aaaaagcttc atggccttta taaaaaggaa ctatccaata cctcgccaga accaagtaac
      781 agtattttac ggggcacaaa tcaagaacaa taagacagga ctgtaaagat ggacgcatag
      841 atcccccctt tagtaagatt tttcaagagt agtctcaaat aaacagaatg aaggattgaa
      901 ggttttattc aataaataat tggacagaaa gttattgcac agttttaaca acaacaattt
      961 caaaatttca atttatgaca tattgaccga cataattcag tcatttctaa tattcaaata
     1021 acaagacacc ttgtataaat gaatccacaa gcgtatatga acaaccaaat tttatgacta
     1081 actgtgggtc gtgtactttt gacgattgag tcatttttac caattaggta attgaatttt
     1141 actaatttgt cattaaccgc gctggccatc ccaacaacaa caacaacaat caatttaaaa
     1201 acttgacaag gcaaaaaaaa aaaatttcat ctttgaaaaa tttttcttcc ttttcttctt
     1261 ctgttcttta cataacagct atcaagaata atcaattaat aatgtcacaa caacaagata
     1321 aacaacctag tgaaaataaa ttatggggtg gtcgtttcac cggtgccact gatccattga
     1381 tggatttgta taatgcttca ttaccttatg ataaagttat gtatgatgct gatttaactg
     1441 gtactaaagt atacacacaa ggattaaaca aattaggatt aattaccact gaagaattga
     1501 atttgattca tcaaggatta gaacaaatca gacaagaatg gcatgataat aaattcatta
     1561 ttaaggctgg tgatgaggat attcatactg ctaatgaacg tcgattagga gaaatcattg
     1621 ggaaaaacat ttctggtaaa gttcacactg gtagatcaag aaatgatcaa gttgctaccg
     1681 atatgagaat tttcgttcgt gaatcattac ttaatttaag taaaatttta catcaattca
     1741 tcacggcaat tcttgaacga gcacataaag aaatcgatgt attaatgcct ggttatactc
     1801 atttacaaaa agctcaacct attagatggg cccattggtt aagttcatat gcaacttatt
     1861 tcaccgaaga ttataaacgt ttacaagaaa ttataactcg tgttaaccaa tctccattag
     1921 gatctggtgc tttagcaggt catccttatg gtattgatcg tgaattttta gctaaaggat
     1981 taggatttga tggtgtcatt gggaattctt taactgcagt ttcagatcgt gattttgttg
     2041 ttgaaagttt attttggagt acattattta tgaatcatat ttctcgattc tcagaagatc
     2101 taattattta ttctagtggt gaatttgggt tcataaaatt agctgatgct tattctactg
     2161 gttcttcatt gatgccacaa aagaaaaacc ctgactcatt agaattatta cgtgggaaaa
     2221 gtggtagagt atttggtcag ttatctggat ttttaatgtc gatcaaatct attccatcta
     2281 cttataataa agatatgcaa gaggataaag aaccattatt tgatgcattg actacagtag
     2341 aacattcaat tttaattgct actggtgtga tttctacttt actgattgat aaacaaaata
     2401 tggaaaaagc tttaactatg gatatgttgg ctactgattt agctgattat ttagttagaa
     2461 aaggagttcc atttagagaa actcatcata tatctggtga atgtgttaga aaagctgaag
     2521 aagaaaaatt atctggaatt gatcaattat cttttgaaca atttcaacaa attgattctc
     2581 gttttgaaaa agacgttatg gaaacttttg attttgaagc tagtgtggaa agaagagatg
     2641 ctcttggtgg tactgctaaa agtgccgttt taaaacaatt agaaaattta aaatcaatct
     2701 taagttaatt tataaatagt catataataa tcacagtatt gtttaactta cataaaatat
     2761 aatcaattaa cacagagata ccttgtacta caatagttta aacgagctcg aattcatcga
     2821 tgatatcaga tccactagtg gcctatgcgg ccgcggatct gccggtctcc ctatagtgag
     2881 tcgtattaat ttcgataagc caggttaacc tgcattaatg aatcggccaa cgcgcgggga
     2941 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3001 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3061 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3121 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3181 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3241 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3301 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     3361 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3421 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3481 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3541 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     3601 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3661 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3721 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3781 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3841 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3901 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3961 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4021 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4081 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4141 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4201 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4261 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4321 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4381 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4441 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4501 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4561 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4621 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4681 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4741 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4801 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4861 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     4921 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     4981 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     5041 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     5101 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atggacatat
     5161 tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac atacgattta
     5221 ggtgacacta ta
//
