LOCUS       40924_18661        7817 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter probe vector pEvoGlowRed, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Krol JE, Rogers LM, Krone SM, Top EM.
  TITLE     Dual reporter system for in situ detection of plasmid transfer under
            aerobic and anaerobic conditions
  JOURNAL   Appl. Environ. Microbiol. 76 (13), 4553-4556 (2010)
  PUBMED    20453134
REFERENCE   2  (bases 1 to 7817)
  AUTHORS   Krol JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2010) Biological Sciences, University of Idaho, 
            P.O. Box 445031, Moscow, ID 83844, USA
REFERENCE   3  (bases 1 to 7817)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7817)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2010"; volume: "76"; issue: "13"; 
            pages: "4553-4556"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAR-2010) Biological Sciences, University of Idaho, P.O. Box 
            445031, Moscow, ID 83844, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             413..430
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      497..544
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1420..2079)
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      complement(2080..2849)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     promoter        4242..4344
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             4345..4977
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQF"
     protein_bind    complement(5113..5134)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(5696..6487)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        6871..6889
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6890..6914
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6922..6938
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6956..6974
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             7036..7710
                     /codon_start=1
                     /label=DsRed1
                     /note="wild-type DsRed"
                     /translation="MRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     promoter        complement(7736..7754)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7761..7777)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             7789..7811
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tatggcgtcg ttccagtcgt tcggcatccc gggccagctg gaagtcatca agaaggcgct
       61 ggatcacgtg cgcgtcggcg tggtcatcac cgatcccgcg ctggaagata acccgatcgt
      121 ctacgtgaac cagggcttcg tgcagatgac cggctacgag accgaggaaa tcctgggcaa
      181 gaacgcgcgc ttcctccagg ggaagcacac cgatccggcg gaagtggaca acatccgcac
      241 cgcgctgcaa aataaagaac cggtcaccgt gcagatccag aactacaaga aggacggcac
      301 gatgttctgg aacgaactga acatcgatcc gatggaaatc gaggataaga cgtatttcgt
      361 cggcatccag aacgacatca ccaagcagaa ggaatatgaa aagctgctcg agcaccacca
      421 ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
      481 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
      541 tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca
      601 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
      661 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
      721 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
      781 cccaaaaaac ttgattaggg tgatggttca ccgcccttcc caacagttgc gcagcctgaa
      841 tggcgaatgg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      901 atcagctcat tttttaacca ataggccgac tgcgatgagt ggcagggcgg ggcgtaattt
      961 ttttaaggca gttattggtg cccttaaacg cctggtgcta cgcctgaata agtgataata
     1021 agcggatgaa tggcagaaat tcgaaagcaa attcgacccg gtcgtcggtt cagggcaggg
     1081 tcgttaaata gccgcttatg tctattgctg gtttaccggt ttattgacta ccggaagcag
     1141 tgtgaccgtg tgcttctcaa atgcctgagg ccagtttgct caggctctcc ccgtggaggt
     1201 aataattgac gatatgatca tttattctgc ctcccagagc ctgataaaaa cggtgaatcc
     1261 gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga
     1321 cgcaacgcgg ggaggcagac aaggtatagg gcggcgaggc ggctacagcc gatagtctgg
     1381 aacagcgcac ttacgggttg ctgcgcaacc caagtgctac cggcgcggca gcgtgacccg
     1441 tgtcggcggc tccaacggct cgccatcgtc cagaaaacac ggctcatcgg gcatcggcag
     1501 gcgctgctgc ccgcgccgtt cccattcctc cgtttcggtc aaggctggca ggtctggttc
     1561 catgcccgga atgccgggct ggctgggcgg ctcctcgccg gggccggtcg gtagttgctg
     1621 ctcgcccgga tacagggtcg ggatgcggcg caggtcgcca tgccccaaca gcgattcgtc
     1681 ctggtcgtcg tgatcaacca ccacggcggc actgaacacc gacaggcgca actggtcgcg
     1741 gggctggccc cacgccacgc ggtcattgac cacgtaggcc gacacggtgc cggggccgtt
     1801 gagcttcacg acggagatcc agcgctcggc caccaagtcc ttgactgcgt attggaccgt
     1861 ccgcaaagaa cgtccgatga gcttggaaag tgtcttctgg ctgaccacca cggcgttctg
     1921 gtggcccatc tgcgccacga ggtgatgcag cagcattgcc gccgtgggtt tcctcgcaat
     1981 aagcccggcc cacgcctcat gcgctttgcg ttccgtttgc acccagtgac cgggcttgtt
     2041 cttggcttga atgccgattt ctctggactg cgtggccatg cttatctcca tgcggtaggg
     2101 tgccgcacgg ttgcggcacc atgcgcaatc agctgcaact tttcggcagc gcgacaacaa
     2161 ttatgcgttg cgtaaaagtg gcagtcaatt acagattttc tttaacctac gcaatgagct
     2221 attgcggggg gtgccgcaat gagctgttgc gtacccccct tttttaagtt gttgattttt
     2281 aagtctttcg catttcgccc tatatctagt tctttggtgc ccaaagaagg gcacccctgc
     2341 ggggttcccc cacgccttcg gcgcggctcc ccctccggca aaaagtggcc cctccggggc
     2401 ttgttgatcg actgcgcggc cttcggcctt gcccaaggtg gcgctgcccc cttggaaccc
     2461 ccgcactcgc cgccgtgagg ctcggggggc aggcgggcgg gcttcgcctt cgactgcccc
     2521 cactcgcata ggcttgggtc gttccaggcg cgtcaaggcc aagccgctgc gcggtcgctg
     2581 cgcgagcctt gacccgcctt ccacttggtg tccaaccggc aagcgaagcg cgcaggccgc
     2641 aggccggagg cttttcccca gagaaaatta aaaaaattga tggggcaagg ccgcaggccg
     2701 cgcagttgga gccggtgggt atgtggtcga aggctgggta gccggtgggc aatccctgtg
     2761 gtcaagctcg tgggcaggcg cagcctgtcc atcagcttgt ccagcagggt tgtccacggg
     2821 ccgagcgaag cgagccagcc ggtggccgct cgcggccatc gtccacatat ccacgggctg
     2881 gcaagggagc gcagcgaccg cgcagggcga agcccggaga gcaagcccgt agggcgccgc
     2941 agccgccgta ggcggtcacg actttgcgaa gcaaagtcta gtgagtatac tcaagcattg
     3001 agtggcccgc cggaggcacc gccttgcgct gcccccgtcg agccggttgg acaccaaaag
     3061 ggaggggcag gcatggcggc atacgcgatc atgcgatgca agaagctggc gaaaatgggc
     3121 aacgtggcgg ccagtctcaa gcacgcctac cgcgagcgcg agacgcccaa cgctgacgcc
     3181 agcaggacgc cagagaacga gcactgggcg gccagcagca ccgatgaagc gatgggccga
     3241 ctgcgcgagt tgctgccaga gaagcggcgc aaggacgctg tgttggcggt cgagtacgtc
     3301 atgacggcca gcccggaatg gtggaagtcg gccagccaag aacagcaggc ggcgttcttc
     3361 gagaaggcgc acaagtggct ggcggacaag tacggggcgg atcgcatcgt gacggccagc
     3421 atccaccgtg acgaaaccag cccgcacatg accgcgttcg tggtgccgct gacgcaggac
     3481 ggcaggctgt cggccaagga gttcatcggc aacaaagcgc agatgacccg cgaccagacc
     3541 acgtttgcgg ccgctgtggc cgatctaggg ctgcaacggg gcatcgaggg cagcaaggca
     3601 cgtcacacgc gcattcaggc gttctacgag gccctggagc ggccaccagt gggccacgtc
     3661 accatcagcc cgcaagcggt cgagccacgc gcctatgcac cgcagggatt ggccgaaaag
     3721 ctgggaatct caaagcgcgt tgagacgccg gaagccgtgg ccgaccggct gacaaaagcg
     3781 gttcggcagg ggtatgagcc tgccctacag gccgccgcag gagcgcgtga gatgcgcaag
     3841 aaggccgatc aagcccaaga gacggcccga gaccttcggg agcgcctgaa gcccgttctg
     3901 gacgccctgg ggccgttgaa tcgggatatg caggccaagg ccgccgcgat catcaaggcc
     3961 gtgggcgaaa agctgctgac ggaacagcgg gaagtccagc gccagaaaca ggcccagcgc
     4021 cagcaggaac gcgggcgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     4081 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcgaa
     4141 taaatacctg tgacggaaga tcacttcgca gaataaataa atcctggtgt ccctgttgat
     4201 accgggaagc cctgggccaa cttttggcga aaatgagacg ttgatcggca cgtaagaggt
     4261 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga
     4321 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     4381 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     4441 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     4501 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     4561 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     4621 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     4681 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     4741 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     4801 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     4861 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     4921 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagttttta
     4981 tgcatgcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttatagtcat
     5041 gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga
     5101 tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt
     5161 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     5221 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct
     5281 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc
     5341 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt
     5401 cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa
     5461 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga
     5521 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt
     5581 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac
     5641 gcagacgcgc cgagacagaa cttaatgaat tccgcgaacc ccagagtccc gctcagaaga
     5701 actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa
     5761 gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca
     5821 acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa
     5881 agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat
     5941 cctcgccgtc gggcatccgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct
     6001 gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc
     6061 gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca
     6121 gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca
     6181 ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa
     6241 cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct
     6301 cgtcttggag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc
     6361 cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt
     6421 catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt
     6481 caatcatgcg aaacgatcct catcctgtct cttgatcaga tcttgatccc ctgcgccatc
     6541 agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag
     6601 agggcgcccc agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct
     6661 atcgccatgt aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg
     6721 tccagatagc ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt
     6781 tctacgtgtt ccgcttcctt tagcagccct tgcgccctga gtgcttgcgg cagcgtgaag
     6841 ctagcggaat tgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag
     6901 cggataacaa ttcccctcta gcaggaaaca gctatgacca tgattacgcc aagctattta
     6961 ggtgacacta tagaatactc aagcttgcat gcctgcaggt cgactctaga ggatccccca
     7021 gaggagaaat taagcatgcg gtcttccaag aatgttatca aggagttcat gaggtttaag
     7081 gttcgcatgg aaggaacggt caatgggcac gagtttgaaa tagaaggcga aggagagggg
     7141 aggccatacg aaggccacaa taccgtaaag cttaaggtaa ccaagggggg acctttgcca
     7201 tttgcttggg atattttgtc accacaattt cagtatggaa gcaaggtata tgtcaagcac
     7261 cctgccgaca taccagacta taaaaagctg tcatttcctg aaggatttaa atgggaaagg
     7321 gtcatgaact ttgaagacgg tggcgtcgtt actgtaaccc aggattccag tttgcaggat
     7381 ggctgtttca tctacaaggt caagttcatt ggcgtgaact ttccttccga tggacctgtt
     7441 atgcaaaaga agacaatggg ctgggaagcc agcactgagc gtttgtatcc tcgtgatggc
     7501 gtgttgaaag gagagattca taaggctctg aagctgaaag acggtggtca ttacctagtt
     7561 gaattcaaaa gtatttacat ggcaaagaag cctgtgcagc taccagggta ctactatgtt
     7621 gactccaaac tggatataac aagccacaac gaagactata caatcgttga gcagtatgaa
     7681 agaaccgagg gacgccacca tctgttcctt taaggggtac cgagctcgaa ttcgccctat
     7741 agtgagtcgt attacaattc actggccgtc gttttaccta gaaataattt tgtttaactt
     7801 taagaaggag atataca
//
