LOCUS       40924_17429        4729 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEnEOmCherryF3SG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Koiwa H.
  TITLE     Arabidopsis thaliana CPL4 is an essential CTD-phosphatase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Koiwa H.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2013) Department of Horticultural Science, Texas A
REFERENCE   3  (bases 1 to 4729)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4729)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-AUG-2013) Department of Horticultural Science, Texas A"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(63..651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(744..1550)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     protein_bind    1673..1772
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1882..2551
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     regulatory      2555..2625
                     /label=derived from Tobacco Mosaic Virus
                     /note="derived from Tobacco Mosaic Virus"
                     /regulatory_class="other"
     CDS             2646..3353
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    3360..3422
                     /label=3xFLAG tag
                     /note="3xFLAG tag"
     CDS             3423..3536
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             3543..3563
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             3570..3590
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     terminator      4000..4252
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    complement(4273..4372)
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(4422..4449)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(4541..4627)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
       61 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      121 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      181 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      241 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      301 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      361 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      421 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      481 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      541 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      601 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      661 ttgatctttt ctacggggtc tgacgctcag tggaacgggg cccaatctga ataatgttac
      721 aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
      781 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
      841 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
      901 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
      961 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc
     1021 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     1081 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
     1141 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
     1201 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgtttttcc ggggatcgca
     1261 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     1321 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
     1381 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa gcgatagatt
     1441 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
     1501 atgttggaat ttaatcgcgg cctcgacgtt tcccgttgaa tatggctcat aacacccctt
     1561 gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt
     1621 gcaatgtaac atcagagatt ttgagacacg ggccagagct gcagctggat ggcaaataat
     1681 gattttattt tgactgatag tgacctgttc gttgcaacaa attgataagc aatgctttct
     1741 tataatgcca actttgtaca agaaagctgg gtctagatta gcttgcatgc ctgcaggtcg
     1801 aagcttgaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
     1861 ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
     1921 cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg
     1981 cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga
     2041 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
     2101 aaccacgtct tcaaagcaag tggattgatg tgataacatg gtggagcacg acactctcgt
     2161 ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca
     2221 acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
     2281 caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa
     2341 ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
     2401 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
     2461 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
     2521 tatataagga agttcatttc atttggagag gtacgtattt ttacaacaat taccaacaac
     2581 aacaacaaac aacaacaaca ttacatttta cattctacaa ctacatctag tcgacccaca
     2641 gatctatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
     2701 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     2761 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     2821 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
     2881 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     2941 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     3001 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     3061 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     3121 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     3181 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     3241 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     3301 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtactctg
     3361 actacaaaga ccatgacggt gattataaag atcatgacat cgattacaag gatgacgatg
     3421 acatggacga gaagaccacc gggtggcggg gcggccacgt tgtggagggt ctcgctggcg
     3481 agctggagca gctcagggcc cgcttggagc accatcccca ggggcaacgc gagcctatcg
     3541 atgaaaatct gtacttccag gggatcgatg agaacctgta ctttcagggg ggtagtaccc
     3601 cggccgtgac gacctacaag ctggtgatca acggcaagac cctgaagggc gagaccacaa
     3661 ccaaggccgt ggacgctgaa accgcagaga aggccttcaa gcagtacgcc aacgacaatg
     3721 gcgtggacgg agtgtggacc tacgacgatg ccaccaagac cttcaccgtg accgaggtta
     3781 acacccccgc agtcaccaca tacaaactgg tgatcaacgg aaaaacgctg aagggcgaaa
     3841 ctacgaccaa ggctgtggac gcggagacgg ccgaaaaggc gttcaaacag tatgccaacg
     3901 ataacggtgt agacggcgtc tggacatatg acgacgcgac aaagactttc actgtcactg
     3961 aataggcggc cgccaccgcg gtggagctcg aatttccccg atcgttcaaa catttggcaa
     4021 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     4081 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     4141 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     4201 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta
     4261 ggttccggcg ccagcctgct tttttgtaca aagttggcat tataaaaaag cattgcttat
     4321 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tggggcccga
     4381 gcttaagtaa ctaactaaca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga
     4441 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc
     4501 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
     4561 cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt
     4621 ttatttgatg cctggcagtt ccctactctc gcttagtagt tagacgtccc cgagatccat
     4681 gctagcggta atacggttat ccacagaatc aggggataac gcaggaaag
//
