LOCUS       40924_17424        5580 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEnEOimCherryF3SGThsp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Koiwa H.
  TITLE     Arabidopsis thaliana CPL4 is an essential CTD-phosphatase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   Koiwa H.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2013) Department of Horticultural Science, Texas A
REFERENCE   3  (bases 1 to 5580)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5580)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-AUG-2013) Department of Horticultural Science, Texas A"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(63..651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(744..1550)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     protein_bind    1673..1772
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1882..2551
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     regulatory      2555..2625
                     /label=derived from Tobacco Mosaic Virus
                     /note="derived from Tobacco Mosaic Virus"
                     /regulatory_class="other"
     intron          2642..3236
                     /note="derived from EF1a"
     CDS             3249..3956
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    3963..4025
                     /label=3xFLAG tag
                     /note="3xFLAG tag"
     CDS             4026..4139
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             4146..4166
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             4173..4193
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     terminator      4578..4826
                     /label=HSP terminator
                     /note="efficient transcription terminator from the
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     terminator      4851..5103
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    complement(5124..5223)
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(5273..5300)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(5392..5478)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
       61 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      121 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      181 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      241 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      301 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      361 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      421 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      481 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      541 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      601 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      661 ttgatctttt ctacggggtc tgacgctcag tggaacgggg cccaatctga ataatgttac
      721 aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
      781 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
      841 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
      901 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
      961 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc
     1021 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     1081 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
     1141 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
     1201 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgtttttcc ggggatcgca
     1261 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     1321 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
     1381 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa gcgatagatt
     1441 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
     1501 atgttggaat ttaatcgcgg cctcgacgtt tcccgttgaa tatggctcat aacacccctt
     1561 gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt
     1621 gcaatgtaac atcagagatt ttgagacacg ggccagagct gcagctggat ggcaaataat
     1681 gattttattt tgactgatag tgacctgttc gttgcaacaa attgataagc aatgctttct
     1741 tataatgcca actttgtaca agaaagctgg gtctagatta gcttgcatgc ctgcaggtcg
     1801 aagcttgaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
     1861 ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
     1921 cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg
     1981 cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga
     2041 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
     2101 aaccacgtct tcaaagcaag tggattgatg tgataacatg gtggagcacg acactctcgt
     2161 ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca
     2221 acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
     2281 caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa
     2341 ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
     2401 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
     2461 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
     2521 tatataagga agttcatttc atttggagag gtacgtattt ttacaacaat taccaacaac
     2581 aacaacaaac aacaacaaca ttacatttta cattctacaa ctacatctag tcgactctga
     2641 ggtaagcttt ttcgtcacca tcttcgatct gcttctttct cttctgttga tctgtgttga
     2701 atctgtttcg atccttctcg tttgtttgtt gtaatctctt ggattcgatt ctctttgttt
     2761 atagatcgtt tggaatttct gatctgtggt gtaaagtttt atcgatttag gagaacaaag
     2821 ttttacagtg ccatcgtatt gttttttttc ttcatgccag ctgatgataa actatatgat
     2881 cgatctggtt ctgaaatttt tgtattgttc aatggattgt tgattggatc ttttgatgta
     2941 tgtgatatta attgtcgatt tgaattgtga ttaggtaatt ctggagcagc ataaacaatc
     3001 aattgattca ttcttcttga taccaagttg tggaaactct aggattgttt gctgtatatc
     3061 tccagaattg ctgttttgat tgaatttagg tcgcttagct cagttgatag agcaccacat
     3121 tttttgtggt agaaatcggt ttgaatccga tagcggcttt taacaatgat tgttttttgt
     3181 gttcataagt ctactgtttt tcttgattca atatttgatt gttcttttta ttgcagattt
     3241 gtagatctat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     3301 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     3361 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     3421 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     3481 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     3541 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     3601 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     3661 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     3721 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     3781 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     3841 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     3901 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtact
     3961 ctgactacaa agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg
     4021 atgacatgga cgagaagacc accgggtggc ggggcggcca cgttgtggag ggtctcgctg
     4081 gcgagctgga gcagctcagg gcccgcttgg agcaccatcc ccaggggcaa cgcgagccta
     4141 tcgatgaaaa tctgtacttc caggggatcg atgagaacct gtactttcag gggggtagta
     4201 ccccggccgt gacgacctac aagctggtga tcaacggcaa gaccctgaag ggcgagacca
     4261 caaccaaggc cgtggacgct gaaaccgcag agaaggcctt caagcagtac gccaacgaca
     4321 atggcgtgga cggagtgtgg acctacgacg atgccaccaa gaccttcacc gtgaccgagg
     4381 ttaacacccc cgcagtcacc acatacaaac tggtgatcaa cggaaaaacg ctgaagggcg
     4441 aaactacgac caaggctgtg gacgcggaga cggccgaaaa ggcgttcaaa cagtatgcca
     4501 acgataacgg tgtagacggc gtctggacat atgacgacgc gacaaagact ttcactgtca
     4561 ctgaataggc ggccgcatat gaagatgaag atgaaatatt tggtgtgtca aataaaaagc
     4621 ttgtgtgctt aagtttgtgt ttttttcttg gcttgttgtg ttatgaattt gtggcttttt
     4681 ctaatattaa atgaatgtaa gatctcatta taatgaataa acaaatgttt ctataatcca
     4741 ttgtgaatgt tttgttggat ctcttctgca gcatataact actgtatgtg ctatggtatg
     4801 gactatggaa tatgattaaa gataagccgc ggtggagctc gaatttcccc gatcgttcaa
     4861 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     4921 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
     4981 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
     5041 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
     5101 atcgggaatt aggttccggc gccagcctgc ttttttgtac aaagttggca ttataaaaaa
     5161 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     5221 ttggggcccg agcttaagta actaactaac aggaagagtt tgtagaaacg caaaaaggcc
     5281 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     5341 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     5401 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     5461 agcctttcgt tttatttgat gcctggcagt tccctactct cgcttagtag ttagacgtcc
     5521 ccgagatcca tgctagcggt aatacggtta tccacagaat caggggataa cgcaggaaag
//
