LOCUS       40924_17419        5836 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEnEOiLUCThsp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Fukudome A, Aksoy E, Wu X, Kumar K, Jeong IS, May K, Russell WK, 
            Koiwa H.
  TITLE     Arabidopsis CPL4 is an essential C-terminal domain phosphatase that 
            suppresses xenobiotic stress responses
  JOURNAL   Plant J. 80 (1), 27-39 (2014)
  PUBMED    25041272
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Koiwa H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2013) Department of Horticultural Science, Texas A
REFERENCE   3  (bases 1 to 5836)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5836)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2014"; volume: "80"; issue: "1"; pages: "27-39"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-AUG-2013) Department of Horticultural Science, Texas A"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(63..651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(744..1550)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     protein_bind    1673..1772
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      1791..2455
                     /label=Arabidopsis UBQ1 promoter
                     /note="Arabidopsis UBQ1 promoter"
                     /regulatory_class="promoter"
     regulatory      1798..2551
                     /label=enhanced cauliflower mosaic virus 35S promoter EL2
                     /note="enhanced cauliflower mosaic virus 35S promoter EL2"
                     /regulatory_class="promoter"
     misc_feature    2555..2625
                     /label=Tobacco mosaic virus omega
                     /note="Tobacco mosaic virus omega"
     intron          2642..3236
                     /note="EF1a-intron"
     CDS             3172..4821
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      4834..5082
                     /label=HSP terminator
                     /note="efficient transcription terminator from the
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     terminator      5107..5359
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    complement(5380..5479)
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(5529..5556)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(5648..5734)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
       61 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      121 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      181 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      241 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      301 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      361 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      421 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      481 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      541 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      601 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      661 ttgatctttt ctacggggtc tgacgctcag tggaacgggg cccaatctga ataatgttac
      721 aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
      781 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
      841 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
      901 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
      961 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc
     1021 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     1081 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
     1141 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
     1201 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgtttttcc ggggatcgca
     1261 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     1321 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
     1381 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa gcgatagatt
     1441 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
     1501 atgttggaat ttaatcgcgg cctcgacgtt tcccgttgaa tatggctcat aacacccctt
     1561 gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt
     1621 gcaatgtaac atcagagatt ttgagacacg ggccagagct gcagctggat ggcaaataat
     1681 gattttattt tgactgatag tgacctgttc gttgcaacaa attgataagc aatgctttct
     1741 tataatgcca actttgtaca agaaagctgg gtctagaact agagcttgat gggatatttc
     1801 acaaattgaa catagactac agaattttag aaaacaaact ttctctctct tatctcacct
     1861 ttatctttta gagagaaaaa gttcgatttc cggttgaccg gaatgtatct ttgtttcttt
     1921 tgttttgtaa catatttcgt tttccgattt agatcggatc tccttttccg ttttgtcgga
     1981 ccttcttccg gtttatccgg atctaataat atccatctta gacttagcta agtttggatc
     2041 tgttttttgg ttagctcttg tcaatcgcct catcatcagc aagaaggtga aatttttgga
     2101 caaataaatc ttagaatcat gtagtgtctt tggacctcgg gaatgataga accgatttgt
     2161 tatagctact ctatgtatca gaccctgacc aagatccaac aatctcatag gttttgtgca
     2221 tatgaaacct tcgactaacg agaagtggtc ttttaatgag agagatatct aaaatgttat
     2281 cttaaaagcc cactcaaatc tcaaggcata aggtagaaat gcaaatttgg aaagtgggct
     2341 gggccttttg tggtaaagac ctgtaaccta gcccaatatt agcaaaaccc tagacgcgta
     2401 cattgacata tataaacccg cctcctcctt gtttagggtt tctacgtgag agaagtacgt
     2461 atttttacaa caattaccaa caacaacaac aaacaacaac aacattacat tttacattct
     2521 acaactacat ctagtcgact ctgaggtaag ctttttcgtc accatcttcg atctgcttct
     2581 ttctcttctg ttgatctgtg ttgaatctgt ttcgatcctt ctcgtttgtt tgttgtaatc
     2641 tcttggattc gattctcttt gtttatagat cgtttggaat ttctgatctg tggtgtaaag
     2701 ttttatcgat ttaggagaac aaagttttac agtgccatcg tattgttttt tttcttcatg
     2761 ccagctgatg ataaactata tgatcgatct ggttctgaaa tttttgtatt gttcaatgga
     2821 ttgttgattg gatcttttga tgtatgtgat attaattgtc gatttgaatt gtgattaggt
     2881 aattctggag cagcataaac aatcaattga ttcattcttc ttgataccaa gttgtggaaa
     2941 ctctaggatt gtttgctgta tatctccaga attgctgttt tgattgaatt taggtcgctt
     3001 agctcagttg atagagcacc acattttttg tggtagaaat cggtttgaat ccgatagcgg
     3061 cttttaacaa tgattgtttt ttgtgttcat aagtctactg tttttcttga ttcaatattt
     3121 gattgttctt tttattgcag atttgtttga cagtctctaa ccatgggatc tatggaagac
     3181 gccaaaaaca taaagaaagg cccggcgcca ttctatcctc tagaggatgg aaccgctgga
     3241 gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca
     3301 gatgcacata tcgaggtgaa catcacgtac gcggaatact tcgaaatgtc cgttcggttg
     3361 gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa
     3421 aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg
     3481 cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgaacat ttcgcagcct
     3541 accgtagtgt ttgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaaatta
     3601 ccaataatcc agaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg
     3661 atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtacca
     3721 gagtcctttg atcgtgacaa aacaattgca ctgataatga attcctctgg atctactggg
     3781 ttacctaagg gtgtggccct tccgcataga actgcctgcg tcagattctc gcatgccaga
     3841 gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc
     3901 catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc
     3961 ttaatgtata gatttgaaga agagctgttt ttacgatccc ttcaggatta caaaattcaa
     4021 agtgcgttgc tagtaccaac cctattttca ttcttcgcca aaagcactct gattgacaaa
     4081 tacgatttat ctaatttaca cgaaattgct tctgggggcg cacctctttc gaaagaagtc
     4141 ggggaagcgg ttgcaaaacg cttccatctt ccagggatac gacaaggata tgggctcact
     4201 gagactacat cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt
     4261 aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc
     4321 gttaatcaga gaggcgaatt atgtgtcaga ggacctatga ttatgtccgg ttatgtaaac
     4381 aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata
     4441 gcttactggg acgaagacga acacttcttc atagttgacc gcttgaagtc tttaattaaa
     4501 tacaaaggat atcaggtggc ccccgctgaa ttggaatcga tattgttaca acaccccaac
     4561 atcttcgacg cgggcgtggc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc
     4621 gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc
     4681 agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg
     4741 aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag
     4801 aagggcggaa agtccaaatt gtaagcggcc gcatatgaag atgaagatga aatatttggt
     4861 gtgtcaaata aaaagcttgt gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat
     4921 gaatttgtgg ctttttctaa tattaaatga atgtaagatc tcattataat gaataaacaa
     4981 atgtttctat aatccattgt gaatgttttg ttggatctct tctgcagcat ataactactg
     5041 tatgtgctat ggtatggact atggaatatg attaaagata agccgcggtg gagctcgaat
     5101 ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     5161 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     5221 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     5281 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     5341 tcatctatgt tactagatcg ggaattaggt tccggcgcca gcctgctttt ttgtacaaag
     5401 ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     5461 aaaataaaat cattatttgg ggcccgagct taagtaacta actaacagga agagtttgta
     5521 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     5581 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     5641 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     5701 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgct
     5761 tagtagttag acgtccccga gatccatgct agcggtaata cggttatcca cagaatcagg
     5821 ggataacgca ggaaag
//
