LOCUS       40924_17414        4715 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEnEOi9mycThsp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Koiwa H.
  TITLE     Arabidopsis thaliana CPL4 is an essential CTD-phosphatase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Koiwa H.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-AUG-2013) Department of Horticultural Science, Texas A
REFERENCE   3  (bases 1 to 4715)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4715)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-AUG-2013) Department of Horticultural Science, Texas A"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(63..651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(744..1550)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     protein_bind    1673..1772
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1884..2551
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     regulatory      2555..2625
                     /label=derived from Tobacco Mosaic Virus Omega sequence
                     /note="derived from Tobacco Mosaic Virus Omega sequence"
                     /regulatory_class="other"
     intron          2646..3240
                     /note="derived from EF1a"
     CDS             3307..3336
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             3343..3372
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3379..3408
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3427..3456
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3463..3492
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3499..3528
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3547..3576
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3583..3612
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3619..3648
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      3713..3961
                     /label=HSP terminator
                     /note="efficient transcription terminator from the
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     terminator      3986..4238
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    complement(4259..4358)
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(4408..4435)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(4527..4613)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
       61 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      121 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      181 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      241 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      301 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      361 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      421 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      481 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      541 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      601 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      661 ttgatctttt ctacggggtc tgacgctcag tggaacgggg cccaatctga ataatgttac
      721 aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
      781 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
      841 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
      901 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
      961 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc
     1021 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     1081 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
     1141 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
     1201 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgtttttcc ggggatcgca
     1261 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     1321 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
     1381 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa gcgatagatt
     1441 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
     1501 atgttggaat ttaatcgcgg cctcgacgtt tcccgttgaa tatggctcat aacacccctt
     1561 gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt
     1621 gcaatgtaac atcagagatt ttgagacacg ggccagagct gcagctggat ggcaaataat
     1681 gattttattt tgactgatag tgacctgttc gttgcaacaa attgataagc aatgctttct
     1741 tataatgcca actttgtaca agaaagctgg gtctagatta gcttgcatgc ctgcaggtcg
     1801 aagcttgcca acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca
     1861 gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc
     1921 ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt
     1981 ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc
     2041 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
     2101 ccaaccacgt cttcaaagca agtggattga tgtgaacatg gtggagcacg acactctcgt
     2161 ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca
     2221 acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
     2281 caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa
     2341 ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
     2401 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
     2461 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
     2521 tatataagga agttcatttc atttggagag gtacgtattt ttacaacaat taccaacaac
     2581 aacaacaaac aacaacaaca ttacatttta cattctacaa ctacatctag tcgatcgact
     2641 ctgaggtaag ctttttcgtc accatcttcg atctgcttct ttctcttctg ttgatctgtg
     2701 ttgaatctgt ttcgatcctt ctcgtttgtt tgttgtaatc tcttggattc gattctcttt
     2761 gtttatagat cgtttggaat ttctgatctg tggtgtaaag ttttatcgat ttaggagaac
     2821 aaagttttac agtgccatcg tattgttttt tttcttcatg ccagctgatg ataaactata
     2881 tgatcgatct ggttctgaaa tttttgtatt gttcaatgga ttgttgattg gatcttttga
     2941 tgtatgtgat attaattgtc gatttgaatt gtgattaggt aattctggag cagcataaac
     3001 aatcaattga ttcattcttc ttgataccaa gttgtggaaa ctctaggatt gtttgctgta
     3061 tatctccaga attgctgttt tgattgaatt taggtcgctt agctcagttg atagagcacc
     3121 acattttttg tggtagaaat cggtttgaat ccgatagcgg cttttaacaa tgattgtttt
     3181 ttgtgttcat aagtctactg tttttcttga ttcaatattt gattgttctt tttattgcag
     3241 atttgtttga cagtctctaa gtcgaccatg gaggagtact cggaattccg ctctagaact
     3301 agtggtgaac aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa gctaatctcc
     3361 gaggaagact tgaacggtga acaaaaatta atctcagaag aagacttgaa cggatcctct
     3421 agaggtgaac aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa gctaatctcc
     3481 gaggaagact tgaacggtga acaaaaatta atctcagaag aagacttgaa cggatcctct
     3541 agaggtgaac aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa gctaatctcc
     3601 gaggaagact tgaacggtga acaaaaatta atctcagaag aagacttgaa cggatccact
     3661 agtggatccc ccgggctgac gcgtgaaagg ccttagttag atagcggccg catatgaaga
     3721 tgaagatgaa atatttggtg tgtcaaataa aaagcttgtg tgcttaagtt tgtgtttttt
     3781 tcttggcttg ttgtgttatg aatttgtggc tttttctaat attaaatgaa tgtaagatct
     3841 cattataatg aataaacaaa tgtttctata atccattgtg aatgttttgt tggatctctt
     3901 ctgcagcata taactactgt atgtgctatg gtatggacta tggaatatga ttaaagataa
     3961 gccgcggtgg agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag
     4021 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     4081 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     4141 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     4201 taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattaggtt ccggcgccag
     4261 cctgcttttt tgtacaaagt tggcattata aaaaagcatt gcttatcaat ttgttgcaac
     4321 gaacaggtca ctatcagtca aaataaaatc attatttggg gcccgagctt aagtaactaa
     4381 ctaacaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
     4441 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
     4501 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
     4561 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
     4621 gcagttccct actctcgctt agtagttaga cgtccccgag atccatgcta gcggtaatac
     4681 ggttatccac agaatcaggg gataacgcag gaaag
//
