LOCUS V019347 7454 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019347 VERSION V019347 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 683..2134 /label="Zdhhc1(NM_001379007)" /note="Zdhhc1(NM_001379007)" /gene="Zdhhc1" CDS 2141..2206 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" misc_feature 2230..2818 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 2884..2932 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 3134..3562 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3576..3905 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3972..4766 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4942..5063 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5112..5128) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5136..5152 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5160..5190) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5205..5226 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5514..6102) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6273..7133) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7134..7238) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccgcca ccatgaacat ctgcaacaaa ccctccaaca agacagcccc 721 cgagaagagt gtgtggacag cgccctcaca ggacagtggg ccttccccag aactgcaggg 781 ccagcgctct cgaaggaatg gatggagctg gccgccccac ccgctccaga ttgtggcctg 841 gctgctctac ctcttcttcg cggtgattgg ctttggggtc cttgttcccc tcttgcctca 901 ccactgggtg cctgctggct atgcttgcat gggggccatc tttgctggcc accttgtggt 961 gcacctgact gctgtctcca tcgatccagc agatgccaat gtgcgggaca agagctactc 1021 tgggcccctg cccatcttca accgcagcca gcatgcgcat gtcattgaag acctgcactg 1081 caacctatgc gacgtggacg tgagtgcgcg atccaaacac tgcagcgcct gcaataagtg 1141 cgtatgcggc ttcgatcacc attgcaagtg gctcaacaac tgcgtgggcg agaggaacta 1201 tcggctcttt ctacacagtg tggcatctgc tttattgggt gtcctgctcc tggtgctggt 1261 ggccacttat gtcttcgtgg agttctttgt caaccccatg cgacttcgta ccaaccaaca 1321 ctttgaagtc ttgaagaacc acacggatgt gtggtttgtg ttcttgcctg ctgcccctgt 1381 ggagacccag gctcctgcca tcctggccct ggccgccctg ctcatccttc tgggcctgct 1441 ttctacagcc ctgcttggcc acctgctctg cttccacatt tatctaatgt ggcacaagct 1501 caccacctat gaatacatcg tgcagcaccg tccagctcag gaagcaaagg agacccacaa 1561 ggagctggag tcatgccccc gcaaggtgcg gtccattcag gagatggagt tctacatgag 1621 gaccttcagc catgtgcgtc cagagccctc tggccaggct aggacagcag cattaaatgc 1681 caatccttcc cagtttcttg ccacccaagg ccaagtggaa ccaccactac cctcctcctc 1741 agacacactg gctctgcctc cacggatcca accccagaaa aagaggaagc ggcgagtgta 1801 tagattgccc aggtctgggg tcttggaccg agagctccca ctgcccaggt tacgggagac 1861 tgggactcct agccgccgct ccagctcttc atctgattcc accagtgcca gcccagtgca 1921 tgctggtggc tctgcaggcg cctactactc ggcatcagct gagtccatgg aagagattcc 1981 agtggcccag acgcgcctgg gcagtgccgc actgggtgcc ccaggagcca ggggtcgaga 2041 gtctgggctg gctctacagg cacgttcgcc tgctgttttt gtgagcccga gcagtggcga 2101 gcccggaaca cctggaggcg gcgacggcct gcctctcgag gactacaaag accatgacgg 2161 tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtagt gagggcccga 2221 tatctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 2281 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 2341 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 2401 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 2461 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 2521 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 2581 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca 2641 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 2701 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 2761 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct 2821 ggaaacgggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta 2881 tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc 2941 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa 3001 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg 3061 ctcgcagcca acgtcggggc ggcaggccct gccatagcag atctgcgcag ctggggctct 3121 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 3181 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 3241 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta 3301 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 3361 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 3421 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 3481 tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt 3541 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt 3601 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 3661 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 3721 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 3781 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 3841 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 3901 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag 3961 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg 4021 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt 4081 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc 4141 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt 4201 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag 4261 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg 4321 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag 4381 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg 4441 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc 4501 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca 4561 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc 4621 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg 4681 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct 4741 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa 4801 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg 4861 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg 4921 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc 4981 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 5041 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg 5101 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 5161 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 5221 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 5281 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 5341 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 5401 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 5461 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 5521 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 5581 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 5641 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 5701 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 5761 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 5821 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 5881 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 5941 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 6001 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 6061 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 6121 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 6181 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 6241 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 6301 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 6361 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 6421 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 6481 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 6541 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 6601 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 6661 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 6721 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 6781 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 6841 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 6901 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 6961 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 7021 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 7081 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 7141 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 7201 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 7261 cctgacgtcg acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct 7321 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg 7381 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga 7441 agaatctgct tagg //