LOCUS       V014064                 6947 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014064
VERSION     V014064
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             683..1606
                     /gene="ZDHHC7"
                     /label="Palmitoyltransferase ZDHHC7"
                     /note="Palmitoyltransferase ZDHHC7 from Homo sapiens.
                     Accession#: Q9NXF8"
     CDS             1613..1639
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1643..1669
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1673..1699
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     misc_feature    1723..2311
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    2377..2425
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      2627..3055
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3069..3398
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3465..4256
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4435..4568
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4605..4621)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4629..4645)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4653..4683)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4698..4719)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5007..5595)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5769..6626)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6627..6731)
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccgcca ccatgcagcc atcaggacac aggctccggg acgtcgagca
      721 tcatcctctc ctggctgaaa atgacaacta tgactcttca tcgtcctcct cctccgaggc
      781 tgacgtggct gaccgggtct ggttcatccg tgacggctgc ggcatgatct gtgctgtcat
      841 gacgtggctt ctggtcgcct atgcagactt cgtggtgact ttcgtcatgc tgctgccttc
      901 caaagacttc tggtactctg tggtcaacgg ggtcatcttt aactgcttgg ccgtgcttgc
      961 cctgtcatcc cacctgagaa ccatgctcac cgaccctggg gcagtaccca aaggaaacgc
     1021 tacgaaagaa tacatggaga gcttgcagct gaagcccggg gaagtcatct acaagtgccc
     1081 caagtgctgc tgtattaaac ccgagcgcgc ccaccactgc agtatttgca aaagatgtat
     1141 tcggaaaatg gatcatcact gcccgtgggt gaacaattgt gtaggagaaa agaatcaaag
     1201 attttttgtg ctcttcacta tgtatatagc tctgtcttca gtccatgctc tgatcctttg
     1261 tggatttcag ttcatctcct gtgtccgagg gcagtggact gaatgcagtg atttttcacc
     1321 tccgataact gtaatcctgt tgatcttcct gtgccttgag ggtcttctgt ttttcacttt
     1381 cactgcagtt atgtttggca cccaaatcca ctccatatgc aacgacgaga cggagatcga
     1441 gcgattgaaa agtgagaagc ccacatggga gcggaggctg cgatgggaag ggatgaagtc
     1501 cgtctttggg gggcccccct cactcctctg gatgaatccc tttgtgggct tccgatttag
     1561 gcgactgccc acgagaccca gaaaaggcgg cccggagttc tcagtgctcg agtacccata
     1621 cgacgtacct gattatgcaa cttatcctta tgatgtacct gattatgcta gctatcctta
     1681 tgatgtccca gactacgcat agtgagggcc cgatatctcg acaatcaacc tctggattac
     1741 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     1801 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     1861 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     1921 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     1981 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     2041 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     2101 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     2161 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     2221 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     2281 agtcggatct ccctttgggc cgcctccccg cctggaaacg ggggaggcta actgaaacac
     2341 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa
     2401 cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     2461 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
     2521 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
     2581 cctgccatag cagatctgcg cagctggggc tctagggggt atccccacgc gccctgtagc
     2641 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     2701 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     2761 ccccgtcaag ctctaaatcg gggcatccct ttagggttcc gatttagtgc tttacggcac
     2821 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     2881 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     2941 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgggg
     3001 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     3061 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     3121 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     3181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     3241 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     3301 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     3361 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     3421 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     3481 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     3541 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     3601 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     3661 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     3721 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     3781 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     3841 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     3901 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     3961 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     4021 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     4081 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     4141 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     4201 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     4261 cgggactctg gggttcgcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     4321 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     4381 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     4441 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4501 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     4561 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     4621 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     4681 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     4741 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     4801 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     4861 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4921 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4981 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5041 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5101 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5161 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     5221 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5281 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5341 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5401 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     5461 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5521 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5581 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5641 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     5701 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     5761 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     5821 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     5881 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     5941 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6001 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6061 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6121 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6181 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6241 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6301 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6361 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     6421 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     6481 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     6541 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     6601 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6661 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     6721 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
     6781 ccgatcccct atggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     6841 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     6901 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagg
//
