LOCUS       V039033                 8194 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039033
VERSION     V039033
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..2850
                     /label="ZDHHC5(NM_015457)"
                     /note="ZDHHC5(NM_015457)"
                     /gene="ZDHHC5"
     CDS             2857..2946
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     misc_feature    2970..3558
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3624..3672
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3874..4302
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4316..4645
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4712..5506
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5682..5803
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5852..5868)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5876..5892
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5900..5930)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5945..5966
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6254..6842)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7013..7873)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7874..7978)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgcc cgcagagtct
      721 ggaaagagat tcaaacccag caagtatgtc ccggtctctg cagccgccat cttcctagtg
      781 ggagctacga cactcttctt tgcctttacg tgtccaggac taagcctgta tgtgtcacct
      841 gcagtgccca tctacaatgc aattatgttt ctctttgtgt tggccaactt cagcatggcc
      901 accttcatgg acccagggat tttccctcga gctgaggagg atgaggacaa ggaagatgat
      961 ttccgagctc ccctttacaa aacagtggag ataaagggca tccaggtgcg catgaaatgg
     1021 tgtgccacct gccgctttta ccgtccccct cgatgttccc actgcagtgt ctgtgacaac
     1081 tgtgtggagg aatttgatca tcactgcccc tgggtgaata actgtattgg tcgccggaac
     1141 taccgttatt ttttcctttt cctcctttcc ctgacagccc acattatggg tgtgtttggc
     1201 tttggcctcc tttatgtcct ctaccacata gaggaactct caggggtccg cacggctgtc
     1261 acaatggcag taatgtgtgt ggctggctta ttcttcatcc ctgtagctgg cctcacggga
     1321 tttcacgtgg ttctggtggc caggggacgc acaaccaatg aacaggttac gggtaaattc
     1381 cggggaggtg tgaacccctt caccaatggc tgctgtaaca atgtcagccg tgttctctgc
     1441 agttctccag cacccaggta tttggggaga ccaaagaaag agaagacaat tgtaatcaga
     1501 cctcccttcc ttcgaccaga agtttcagat gggcagataa ctgtgaagat catggataat
     1561 ggcatccagg gagagctgag gagaacaaag tctaagggaa gcctggagat aacagagagc
     1621 cagtctgcag atgctgaacc tccacctcct cctaagccag acctgagccg ttacacaggg
     1681 ttgcgaacac acctcggctt ggctactaat gaggatagta gcttattggc caaggacagc
     1741 cccccgacac ctaccatgta caagtatcgg ccgggttaca gtagcagcag tacgtcagct
     1801 gccatgccgc attcctccag cgccaagttg agtcgtgggg acagcttgaa ggagccaacc
     1861 tcaattgcag agagcagccg tcaccccagc taccgctcag agcccagctt ggaaccagag
     1921 agcttccgtt ctcctacctt tggcaaaagt tttcacttcg atccactatc cagtggctca
     1981 cgctcctcca gcctcaagtc agcccagggc acaggctttg agctgggcca gttgcaatcc
     2041 attcgttcag agggcaccac ctccacctcc tataagagcc tggccaacca gacacgcaat
     2101 ggaagcctat cttatgacag cttgctcaca ccttcagaca gccctgattt tgagtcagtg
     2161 caggcagggc ctgagccaga cccaccttta ggctatacct ctcccttcct gtcagccagg
     2221 ctggcccagc aacgggaagc tgagaggcac ccacgtttgg tgccaactgg cccaacacac
     2281 cgagagccct caccagtccg ttacgacaat ctgtcgcgcc acattgtggc ctctctccag
     2341 gaacgagaga agttgctgcg ccagtcaccc ccactcccgg gccgtgagga agaaccaggc
     2401 ttgggggact caggcattca gtcaacacca ggctcgggcc atgcccctcg tactagttcc
     2461 tcctcagatg attcaaagag atcacctttg ggcaagactc cactgggacg cccagctgtc
     2521 ccccgttttg gcaagccaga tgggctaagg ggccggggag tagggtcccc tgaaccaggc
     2581 ccaacagccc catacctggg ccgatcgatg tcttacagca gccaaaaagc ccaacctggt
     2641 gtctctgaga cagaagaagt ggccttgcag ccattactga cacccaaaga tgaagtacag
     2701 ctgaagacca cctacagcaa atccaacggg cagcccaaga gcttaggctc agcctcccct
     2761 ggcccaggcc agccacctct cagtagcccc acgaggggag gagtcaagaa ggtgtcaggg
     2821 gttggtggta ccacctatga gatttcggtg ctcgaggaac aaaaactaat atcagaggaa
     2881 gatttggaac agaaactaat cagtgaagaa gatttagagc aaaagttaat ttccgaggag
     2941 gacttatagt gagggcccga tatctcgaca atcaacctct ggattacaaa atttgtgaaa
     3001 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     3061 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3121 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     3181 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     3241 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     3301 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     3361 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     3421 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     3481 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     3541 tttgggccgc ctccccgcct ggaaacgggg gaggctaact gaaacacgga aggagacaat
     3601 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg
     3661 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     3721 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     3781 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcag
     3841 atctgcgcag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     3901 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     3961 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4021 taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4081 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4141 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4201 tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt
     4261 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     4321 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4381 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4441 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     4501 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4561 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     4621 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     4681 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     4741 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     4801 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     4861 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     4921 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     4981 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     5041 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     5101 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     5161 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     5221 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     5281 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     5341 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     5401 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     5461 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     5521 ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     5581 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     5641 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     5701 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5761 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     5821 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5881 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     5941 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6001 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6061 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6121 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6181 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6241 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6301 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6361 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6421 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     6481 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6541 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6601 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6661 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     6721 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6781 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6841 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6901 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6961 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7021 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7081 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7141 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7201 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7261 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7321 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7381 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7441 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7501 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7561 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7621 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7681 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7741 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7801 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7861 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7921 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7981 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     8041 gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     8101 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     8161 cttgaccgac aattgcatga agaatctgct tagg
//
