LOCUS       pEnCMV-ZC3H13(human)   11018 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020427
VERSION     V020427
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             683..5773
                     /label="ZC3H13(NM_001382211)"
                     /note="ZC3H13(NM_001382211)"
                     /gene="ZC3H13"
     misc_feature    5794..6382
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    6448..6496
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6698..7126
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7140..7469
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7536..8330
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8506..8627
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8676..8692)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8700..8716
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8724..8754)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8769..8790
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9078..9666)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9837..10697)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10698..10802)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccgcca ccatgtcaaa aattagaagg aaggtcacag tggaaaatac
      721 caagactata tctgatagca catcccgaag acccagtgta tttgagaggc ttggacccag
      781 cactggcagt acagcagaga cacagtgccg taactggctg aagactggca actgcctcta
      841 tggaaacaca tgtagattcg tacatggccc ttcacctcgt ggtaaaggtt atagcagcaa
      901 ttatagaagg tcaccagaaa gacctacagg ggatcttaga gaaagaatga agaacaagcg
      961 ccaagacgtg gacactgagc cccagaaacg aaatacagag gagtcatcct cacctgttag
     1021 gaaagaatct tcaagaggga gacataggga aaaggaagac ataaaaatca ctaaggaaag
     1081 aactccagaa agtgaagaag aaaatgtaga atgggaaact aatagagatg attctgacaa
     1141 tggagatatt aattatgatt atgttcatga attgtcattg gaaatgaagc gtcagaagat
     1201 acagagggaa ttaatgaagc tggaacaaga aaacatggag aagagagaag aaattatcat
     1261 taaaaaggag gtttcaccag aagtggttag atcaaaattg tccccgtcac cttctctaag
     1321 aaagtctagc aaatctccga agcgaaaatc aagcccgaag tcgtcttcag ctagcaagaa
     1381 agataggaag acatctgcag tatcttctcc cctgttggac cagcagagaa attcaaaaac
     1441 caaccaaagt aaaaagaaag gaccacgtac tcctagtcca ccccctccta taccagaaga
     1501 tatcgctctg gggaaaaaat acaaagaaaa atataaagta aaagacagga tagaagaaaa
     1561 aacaagagat ggaaaggaca gaggacgaga ttttgaacga caaagagaaa agagagacaa
     1621 gccaaggtct acttccccag caggacagca tcattctcct atatcttcta gacatcactc
     1681 atcttcctca caatcaggat catctattca aagacattct ccttctcctc gtcgaaaaag
     1741 aactccttca ccatcttatc agcggacact aactccacct ttacgacgct ctgcctctcc
     1801 ttatccttca cattctttgt cgtctcccca gagaaagcag agtcctccaa gacatcgctc
     1861 tccaatgcga gagaaaggga gacatgatca tgaacgaact tcacagtctc atgatcgacg
     1921 ccatgaaagg agggaagata ctaggggcaa acgagacaga gaaaaggact caagagaaga
     1981 acgagaatat gaacaggatc agagctcttc tagagaccac agagatgaca gagaacctcg
     2041 agatggtcgg gatcggagag atgccagaga tactagggac cgaagggaac taagagactc
     2101 cagagacatg cgggactcaa gggagatgag agattatagc agagatacca aagagagccg
     2161 tgatcccaga gattctcggt ccactcgtga tgcccatgac tacagggacc gtgaaggtcg
     2221 agatactcat cgaaaggagg atacatatcc agaagaatcc cggagttatg gccgaaacca
     2281 tttgagagaa gaaagttctc gtacggaaat aaggaatgag tccagaaatg agtctcgaag
     2341 tgaaattaga aatgaccgaa tgggccgaag tagggggagg gttcctgagt tacctgaaaa
     2401 gggaagtcga ggctcaagag gttctcaaat tgatagtcac agtagtaata gcaactatca
     2461 tgacagctgg gaaactcgaa gtagctatcc tgaaagagat agatatcctg aaagagacaa
     2521 cagagatcaa gcaagggatt cttcctttga gagaagacat ggagagcgag accgtcgtga
     2581 caacagagag agagctccta tgttctacac ctcctgctgc ccctatcctt tgccaccagt
     2641 gttctcaggt cctgctgggg ggacagagaa gatcagagat caaagaccaa gctcaccaat
     2701 tcgacatcag ggaaggaatg acgagcttga gcgtgatgaa agaagagagg aacgaagagt
     2761 agacagagtg gatgatagga gagatgaaag ggctagagag agagatcggg aacgagaacg
     2821 agacagggag cgggagagag agagggaacg tgaacgggat cgggaaagag aaaaagagag
     2881 agaactagaa agagagcgtg ctagggaacg ggagagagaa agagaaaaag agagagatcg
     2941 tgaaagggat agagaccgag accacgatcg agagcgggaa agagagaggg aacgagacag
     3001 ggaaaaagaa cgggaacgag aaagagaaga gagagagagg gagagagagc gagaacggga
     3061 gagagagcga gagcgagaac gggaacgaga aagagcgaga gaaagggata aagaacgaga
     3121 acgccaaagg gattgggaag acaaagacaa aggacgagat gaccgcagag aaaagcgaga
     3181 agagatccga gaagatagga atccaagaga tggacatgat gaaagaaaat caaagaagcg
     3241 ctatagaaat gaagggagtc ccagccctag acagtccccg aagcgccggc gtgaacattc
     3301 tccggacagt gatgcctaca acagtggaga tgataaaaat gaaaaacaca gactcttgag
     3361 ccaagttgta cgacctcaag aatctcgttc tcttagtccc tcgcacctca cagaagacag
     3421 acagggtaga tggaaagagg aggatcgtaa accagaaagg aaagagagtt caaggcgcta
     3481 cgaagaacag gaactcaagg agaaagtttc ttctgtagat aaacagagag aacagacaga
     3541 aatcctggaa agctcaagaa tgcgtgcaca ggacattata ggacaccacc agtctgaaga
     3601 tcgagagaca tctgatcgag ctcatgatga aaacaagaag aaagcaaaaa ttcaaaagaa
     3661 accaattaag aaaaagaaag aggatgatgt tggaatagag aggggtaaca tagagacaac
     3721 atctgaagat ggtcaagtat tttcaccaaa aaaaggacag aaaaagaaaa gcattgaaaa
     3781 aaaacgtaaa aaatccaaag gtgattctga tatttctgat gaagaagcag cccagcaaag
     3841 taagaagaaa agaggcccac ggactccccc tataacaact aaagaggaat tggttgaaat
     3901 gtgcaatggt aagaatggta ttctagagga ctcccagaaa aaagaagata cagcattcag
     3961 tgactggtct gatgaggatg tccctgaccg tacagaggtg acagaagcag agcatactgc
     4021 caccgccacg actcctggta gtaccccttc tcctctatct tctcttcttc ctcctccacc
     4081 gcctgtggct actgccactg ctacaactgt gcctgcaact cttgctgcca ctactgctgc
     4141 tgccgccacc tctttcagca catctgccat cactatttcc acctctgcca cccccaccaa
     4201 taccaccaat aatacttttg ccaatgaaga ctcacacaga aaatgccaca gaacacgagt
     4261 agaaaaagta gagacgcctc acgtgactat agaagatgca cagcatcgca agcctatgga
     4321 tcaaaagagg agcagcagcc tcgggagcaa tcggagtaac cgtagtcata cgtctggtcg
     4381 tcttcgctcc ccatccaatg attcagccca tcgaagtgga gatgaccaaa gtggtcgaaa
     4441 gagagtactg cacagtggct caagagatag agaaaaaaca aaaagcctgg aaatcacagg
     4501 agagagaaaa tctaggattg atcagttaaa gcgtggagaa cccagtcgaa gtacttcttc
     4561 agatcgccag gattcaagaa gccatagttc aagaagaagt tctccagagt cagatcgaca
     4621 ggtccattca agatctgggt catttgatag cagagacagg cttcaagaac gagatcgata
     4681 tgaacacgac agagagcgcg agagagagag gagagatacg aggcagagag aatgggaccg
     4741 agatgctgat aaagattggc cacgcaacag ggatcgagat agattgcgag aacgagaacg
     4801 agagagagaa cgagacaaaa ggagagactt ggatagggaa agagagagac taatttctga
     4861 ttctgttgaa agggacaggg acagagacag agacagaact tttgagagtt ctcaaataga
     4921 gtctgtgaaa cgctgtgaag caaaactgga aggtgaacat gaaagggatc tagaaagcac
     4981 ttcccgagac tctctagcct tggataaaga gagaatggat aaagatctgg gatctgtgca
     5041 gggatttgaa gaaacaaata aatccgagag aactgagagt ctggaagcag gagatgacga
     5101 gtccaagtta gatgatgcac attcattagg ctctggtgct ggagaaggat acgagccaat
     5161 cagtgatgac gaactagatg aaattctggc aggtgatgca gaaaagaggg aggaccaaca
     5221 ggatgaggag aagatgccag atcccttaga tgtgatagat gtggattggt ctggtcttat
     5281 gccaaagcat ccaaaagaac cacgagagcc tggggctgca ctcttaaaat tcacacctgg
     5341 agctgttatg ctaagagttg ggatttctaa aaagttggca ggttctgaac tctttgccaa
     5401 agtcaaagaa acatgtcaga gacttttaga aaaacccaaa gatgcagaca atctctttga
     5461 acatgaattg ggggctctca atatggctgc attactacga aaagaagaaa gagcaagtct
     5521 tcttagtaat cttggcccat gttgtaaggc gttgtgcttc agacgggatt ctgcaattcg
     5581 aaagcagctt gttaaaaatg agaagggcac cataaaacaa gcttacacga gtgctccaat
     5641 ggtagacaat gaattacttc gattgagtct tcggttattt aagcggaaga ctacttgcca
     5701 tgctccagga catgaaaaga ctgaagataa taaactttca cagtccagta tccaacagga
     5761 actgtgtgtg tcttaagggc ccgatatctc gacaatcaac ctctggatta caaaatttgt
     5821 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     5881 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     5941 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6001 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6061 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6121 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6181 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     6241 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     6301 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     6361 tccctttggg ccgcctcccc gcctggaaac gggggaggct aactgaaaca cggaaggaga
     6421 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt
     6481 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     6541 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     6601 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     6661 gcagatctgc gcagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     6721 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6781 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6841 gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6901 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6961 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7021 acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc
     7081 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     7141 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7201 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7261 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     7321 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7381 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     7441 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     7501 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     7561 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     7621 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     7681 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     7741 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     7801 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     7861 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     7921 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     7981 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     8041 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     8101 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     8161 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     8221 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     8281 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     8341 ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     8401 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     8461 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     8521 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     8581 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     8641 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     8701 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     8761 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     8821 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8881 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8941 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     9001 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9061 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9121 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9181 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9241 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     9301 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9361 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9421 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9481 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     9541 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9601 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9661 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9721 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     9781 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9841 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9901 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9961 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    10021 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    10081 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    10141 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    10201 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    10261 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    10321 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    10381 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    10441 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    10501 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    10561 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    10621 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    10681 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    10741 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    10801 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
    10861 tatggtcgac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
    10921 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
    10981 aaggcttgac cgacaattgc atgaagaatc tgcttagg
//
