LOCUS       V025381                 7537 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025381
VERSION     V025381
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..2196
                     /label="XIAP(NM_001167)"
                     /note="XIAP(NM_001167)"
                     /gene="XIAP"
     CDS             2203..2229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2233..2259
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2263..2289
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    2313..2901
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    2967..3015
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3217..3645
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3659..3988
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4055..4849
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5025..5146
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5195..5211)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5219..5235
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5243..5273)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5288..5309
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5597..6185)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6356..7216)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7217..7321)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgac ttttaacagt
      721 tttgaaggat ctaaaacttg tgtacctgca gacatcaata aggaagaaga atttgtagaa
      781 gagtttaata gattaaaaac ttttgctaat tttccaagtg gtagtcctgt ttcagcatca
      841 acactggcac gagcagggtt tctttatact ggtgaaggag ataccgtgcg gtgctttagt
      901 tgtcatgcag ctgtagatag atggcaatat ggagactcag cagttggaag acacaggaaa
      961 gtatccccaa attgcagatt tatcaacggc ttttatcttg aaaatagtgc cacgcagtct
     1021 acaaattctg gtatccagaa tggtcagtac aaagttgaaa actatctggg aagcagagat
     1081 cattttgcct tagacaggcc atctgagaca catgcagact atcttttgag aactgggcag
     1141 gttgtagata tatcagacac catatacccg aggaaccctg ccatgtatag tgaagaagct
     1201 agattaaagt cctttcagaa ctggccagac tatgctcacc taaccccaag agagttagca
     1261 agtgctggac tctactacac aggtattggt gaccaagtgc agtgcttttg ttgtggtgga
     1321 aaactgaaaa attgggaacc ttgtgatcgt gcctggtcag aacacaggcg acactttcct
     1381 aattgcttct ttgttttggg ccggaatctt aatattcgaa gtgaatctga tgctgtgagt
     1441 tctgatagga atttcccaaa ttcaacaaat cttccaagaa atccatccat ggcagattat
     1501 gaagcacgga tctttacttt tgggacatgg atatactcag ttaacaagga gcagcttgca
     1561 agagctggat tttatgcttt aggtgaaggt gataaagtaa agtgctttca ctgtggagga
     1621 gggctaactg attggaagcc cagtgaagac ccttgggaac aacatgctaa atggtatcca
     1681 gggtgcaaat atctgttaga acagaaggga caagaatata taaacaatat tcatttaact
     1741 cattcacttg aggagtgtct ggtaagaact actgagaaaa caccatcact aactagaaga
     1801 attgatgata ccatcttcca aaatcctatg gtacaagaag ctatacgaat ggggttcagt
     1861 ttcaaggaca ttaagaaaat aatggaggaa aaaattcaga tatctgggag caactataaa
     1921 tcacttgagg ttctggttgc agatctagtg aatgctcaga aagacagtat gcaagatgag
     1981 tcaagtcaga cttcattaca gaaagagatt agtactgaag agcagctaag gcgcctgcaa
     2041 gaggagaagc tttgcaaaat ctgtatggat agaaatattg ctatcgtttt tgttccttgt
     2101 ggacatctag tcacttgtaa acaatgtgct gaagcagttg acaagtgtcc catgtgctac
     2161 acagtcatta ctttcaagca aaaaattttt atgtctctcg agtacccata cgacgtacct
     2221 gattatgcaa cttatcctta tgatgtacct gattatgcta gctatcctta tgatgtccca
     2281 gactacgcat agtgagggcc cgatatctcg acaatcaacc tctggattac aaaatttgtg
     2341 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     2401 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     2461 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     2521 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     2581 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     2641 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     2701 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     2761 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     2821 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     2881 ccctttgggc cgcctccccg cctggaaacg ggggaggcta actgaaacac ggaaggagac
     2941 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg
     3001 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
     3061 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
     3121 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
     3181 cagatctgcg cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     3241 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     3301 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     3361 ctctaaatcg gggcatccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     3421 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     3481 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     3541 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgggg atttcggcct
     3601 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     3661 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3721 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3781 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3841 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3901 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     3961 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     4021 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     4081 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     4141 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     4201 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     4261 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     4321 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     4381 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     4441 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     4501 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     4561 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     4621 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     4681 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     4741 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     4801 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     4861 gggttcgcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     4921 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     4981 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     5041 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5101 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     5161 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     5221 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     5281 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     5341 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     5401 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     5461 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     5521 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     5581 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     5641 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     5701 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5761 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     5821 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5881 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5941 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6001 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     6061 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6121 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6181 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6241 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6301 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6361 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     6421 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6481 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     6541 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6601 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6661 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     6721 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6781 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6841 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     6901 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     6961 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7021 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7081 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7141 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7201 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7261 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7321 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     7381 atggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     7441 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     7501 aggcttgacc gacaattgca tgaagaatct gcttagg
//
