LOCUS       V044753                11751 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044753
VERSION     V044753
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..6431
                     /label="Wdr90(NM_001163766)"
                     /note="Wdr90(NM_001163766)"
                     /gene="Wdr90"
     CDS             6438..6503
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    6527..7115
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    7181..7229
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7431..7859
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7873..8202
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8269..9063
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9239..9360
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(9409..9425)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9433..9449
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9457..9487)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9502..9523
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9811..10399)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10570..11430)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11431..11535)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatggc cggaggtagc ggcgcgcgcg
      781 agacgcgctg tcgtcagggc tcgcgtcccg ggagtcccgg gtccgaggtc cccgccgcgt
      841 cagtgactgg acctcgtgca gaagcccgtc ccgcgggcgg aggtggaggg tcgcggcgcg
      901 ctgccccaga tcggtgctca ggcggcgtcc cttctgcagc ctggcagcac cctttcctca
      961 atgtattcag acatttcagg gtggatgagt ggaaacggtc cagcaaagag ggagatgtgg
     1021 ctgtggtgac ggacaaggtc ctgaagagcg ctgtgtatcg catccgtggg tctgtatctg
     1081 ccagcaacta catccagctc cccagaacta gcacccagtc tctggggctg actgggcggt
     1141 acctgtatgt gcttttccgg cctgtgccta ccaagcactt tgtcatccac ctggacgtgt
     1201 ccaccgagga cggccaggtc atccgtgtgt ctttctccaa cctctttaag gagttcaagt
     1261 cctccacaac gtggcttcag ttcccctttg tcttcgagac taagacaccc aggagaggtg
     1321 gtgacatttg gtccaagagg gacggcttga atgaaggcct aaaacgggat gacctggcag
     1381 gtgtagctct tcctcgtgcc cgctggacct gcctacagct ggacctgcgt gacatcctga
     1441 tgttctacct gggcaggcac tatagtcatc tcaagagcat caggctgtgt gccagcctgc
     1501 tagtcagaaa cctctacacc agtgatctgt gctttgaccc tgctgtcact gtcactgagg
     1561 cccggcgtgc aaagttgtct gttaacccta tgcctcgaga aatggctttc ccggtgccaa
     1621 aaggggagag ctggcatgac aattacatcc acgttcggtt tccaagtgac tgttccaagg
     1681 tgcctgatga gcaggatgag aagagctgtt cccccccaga agcagtcttc ctaggtcgta
     1741 tgtcacggca cctgcctcat ccaggggtct tgggcaaacc tttgctgagc agtaagtccc
     1801 ctgtggccca ggcatgtagc tctgcattgc catgccaggt cctttcagca tctagtcgcc
     1861 ttccagaggt cagccggacc tacaggtatc gagaagtctc cagtgtaagt gcctccagca
     1921 tccagagcca acgtccttca gttcgcgatg aggttcccga tgcacacacg gtgtccggcg
     1981 agagacacgt tcttgctgac agatcatctg gcgtgcccat ggcccttgag gacattggtt
     2041 cctgtaggct tttcctccca gacccagtcc tgaggctcaa gggcgtcatc ggttttgggg
     2101 gtcacagcac ccaatgggcc ctgtggacca aagatggtgt ggctgttgtt tacccctgcc
     2161 atgcagtcat aattgtcctg cagattgaca ctggacagca gaggtttttc cttggccata
     2221 ccgacaaggt ctctgccctg gcgctcaatg gcagtgatac gctgctagcc tcagctcagg
     2281 tgcagcctcc cagcatggtg cgtctctggg acttccagac tgggagttgc ctgtctctct
     2341 tcagaagccc actgcacacc atctgctccc tcagcttctc tggcagcggg gcactcctct
     2401 gcggtgtggg caaggacaga cacgggagga cggtggtggt ggcctggagt acagagcagg
     2461 cgggcctcgg cggtgaggtc gtggttcttg caaaagtgca cactgacttc gatattcggg
     2521 ccttccaggt ggcctttttt gatgaaacca ggatggcatc atgtggtcgg ggcagcgtga
     2581 ggctctggcg gctgcggggt ggtgttcttc ggtcctgcgc tgtggacctg ggggagtact
     2641 gttcactgga gctcactgac cttgcctttg cacaggcgtt ggatggccac tgtgccccct
     2701 cggccggcac actctttgtg tgcagccata gtggtcacat cctggagatt gaccaccagc
     2761 gcatgtcggt gcagcacgtc cgccgcttgc tgcctgcacg gtcccctggt gctcccctcg
     2821 ccgagaagca gaactttagc gtggggtccg gcatcgctat cagtagcctc agtgtctcta
     2881 ctgccacatg tgctgtgggc tctgaggatg gctacttgcg actctggccc ctggactttt
     2941 cctccgtgct cctagaggcc gaacacgacg gccctgtcag ttcggtctcc ttcagtcccg
     3001 atggcctgcg tgtgctgtcc accaccactt caggccacct gggcttcctg gatgtcccct
     3061 cccgggagta tactgtgttg gcacgctccc acatggcccc agtgttggcg ctttctacag
     3121 aaccgaaccg gggacagatg gccactgtgt cccttgacca cactgtccgt atctgggacc
     3181 tggctaccct acagcagctg tatgacttct cgtcctccga ggacacccct tgtgctgttg
     3241 ctttccatcc cacaatgcca aacttcttct gtggcttcag cagtggggcc gtgcgttcct
     3301 tcagcttgga gagctctgga gtcctggtag aacacacgcg tcaccgaggg gccatcacca
     3361 gcctggtcat cacccttgac ggcagattcc tgttcagctc ctgctctcag ggctccctag
     3421 tccagtacag ctgtgctgac tctcagtgct gcgtcctacg tgtggcagcc aacatggtct
     3481 gtcaggatgg tcgccccaac cccaatattc tggcagtcag tggagacagc tgccggctgg
     3541 cctttgtggg cccctccaag tgcatggtga caattgtgga gtcggcctct ttggatgagc
     3601 tactccgtgt tgatgtcagc accctcaacc tggccagcaa ccacctggac tgggctgtgg
     3661 ctatctgctt cagccccggg aactcaggcc atctgctggt gtccacatcc tctaacaagg
     3721 ttgtggtact ggatgctgtg tcaggacaca ccctccggga gttatctagt gtccgctcca
     3781 gagcctgctg ctccctggct ctcagtgagg atgctcattt gctgctggca gccactgacc
     3841 ggaccattac agtatgggat tatccaacac aggcgaaccc cagctgccag gtatacattg
     3901 gccactcaga gcctgtgcac gctgtagcct tcacacctga tcagctgcag gtcatcagtg
     3961 tgggggatgc tatcttcctc tgggacatcc tggctacccc tgagagagat ggaggtgatc
     4021 atgaagcacc cccagggcat gaggccggct ctagttcggg gcagctggat gacctggcat
     4081 ccggggccag tggactccct cggcagcaag tgcccatacc gtttcaggca ttaccgcctc
     4141 cactgagtag ccatgacagg ctccttgacg gcagtactgg caccttctcc acgtctgatg
     4201 aggaaggact ctgtgaggag aaccatgttt ctgaggcgct cctccaaggc caggccccga
     4261 ccccacatgt gctcgtggac agggaagcca gtggtgctgg ggatgcccct cgggagactg
     4321 cagggagctc ctggacacca gctgagcgtc ccagtcccca cagccacatg agtgaatgga
     4381 gcctgcggag cgggaaggct gggctcccaa cccgcccaga ttcctacaag cacttcacac
     4441 ctcgatataa gacctcacca cgggttaaga gtgtctactt ccctcccatt ggcagggagc
     4501 ggctgcgact gaaggccatt gtgggctaca atgggaatgg gcgggccaac atggtctgga
     4561 ggcccgacac aggcttcttt gcctacactt gtggtcgcct ggtggtagtg gaggacctgc
     4621 attctggcac ccagaggcac tggcttggcc attctcagga gatctctact ctggcactca
     4681 accaggatgg ccagatcttg gcctctgcct cctgctgcgg caacacagct gcccgttgcc
     4741 agatccgaat ttgggatgtt cccaagggcc tttgtcggca cctcctttcc catcatgaca
     4801 cagctgtgca agctctggct ttctcaccag atgatgagtt ccttgtaacg ctgggggact
     4861 atgctgacag gaaccttgcc ctgtggagca tggccaccta tgaactcctg tcatccactc
     4921 gcctcctgga gcctgtgcat ggtgtggcct ttaacccatg ggatgccaat gagctcatct
     4981 gtgtgggcac aaatgccatc accttctggc tcctgcagca tcatggggca gacacctgct
     5041 tccaggtaca ccgggaacca atccccgagg aactgggggc gtccgagttg acctcactct
     5101 gctatggggc cagccctctg ctctactgcg gctccagctc tggccaggtc tgtgtctggg
     5161 acactggcac tggccactgc ttcctggcct gggaggctga cgatggggag atcggggtgc
     5221 tgctgtgttc aggctctaga ctgatcagtg ggagcaacac aaaaaggctg cgcctttggg
     5281 ctgtgggggt tgtgccagag ctaaggcgca aaggctccag tgccagatcc agttctgttt
     5341 tcatggagcg tgagctgacc ctggatgggg ctgttgtgag tgccagtttt gacagtggca
     5401 tggacatggg tgtagtgggt accacagctg gcaccatctg gtacatcagc tggacagagg
     5461 gcactagcac ccgcctcatc agtggccaca ggaccaaggt aaacgaggtg gtcttcagcc
     5521 ccggtgagtc tcactgtgcc acctgtggtg aggatgggag cgtgagggtg tggtctttgg
     5581 ccagcatgga gctggtgatc cagtttcagg tgctgaacca gagctgtctc tgccttgcgt
     5641 ggacgccccc gtcctgtgaa ctcccagagc agcagcaggt ggtggccggc tacagcgatg
     5701 gcacacttcg agtcttcagt atctctcgta cagcaatgga actcaagatg cacccccacc
     5761 ggaccgctct gacagccatc gccttctcca ccgacggtca gaccatccta tctggagata
     5821 aggacggact tgtggctata agccacccct gcacaggaat gaccttccgt gtgctcagtg
     5881 accaccgggg tgctccaatt tctgctatcc agagcacgag caaagaatat ggagacctag
     5941 gggtagaggg tgtagaactg tggctggctg caagtgggga ccaacgtgtc agcatctggg
     6001 tctctgactg gctccgggac cgctgtgaac tcctggaatg gttgagcttt cctgcacctg
     6061 ctgtctcaga ggctccaggc ctcctgcccc cctctcttgc tgccttctgc ccttgggata
     6121 aggcgatact ggtgtgtgtg ggcctgggtg cacatgagga ggtcatcttc tacagcctcc
     6181 gtcagaagca ggtgatacag aagacacctc tgcccttctt tgccatgtct ttgagcctgt
     6241 ccccaggatc tcagctcatg gtggttggtt ttgctgagtg catgatgagg ctcctagact
     6301 gtgcgtcagg gactgcccag gacttggaag gccatgatga ctcggtccac ctgtgtaggt
     6361 tcactccatc tggcagactg ctcttcacag ctgctcacaa cgagatcctg gtgtgggaag
     6421 tcaccgatcc cggatccgac tacaaagacc atgacggtga ttataaagat catgacatcg
     6481 actacaagga tgacgatgac aagtagtgag ggcccgatat ctcgacaatc aacctctgga
     6541 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     6601 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     6661 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     6721 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     6781 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     6841 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     6901 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     6961 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7021 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7081 gacgagtcgg atctcccttt gggccgcctc cccgcctgga aacgggggag gctaactgaa
     7141 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     7201 aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     7261 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     7321 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     7381 aggccctgcc atagcagatc tgcgcagctg gggctctagg gggtatcccc acgcgccctg
     7441 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     7501 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     7561 ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta gtgctttacg
     7621 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     7681 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     7741 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     7801 ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     7861 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     7921 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     7981 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     8041 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8101 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     8161 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     8221 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     8281 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     8341 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     8401 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     8461 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     8521 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     8581 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     8641 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     8701 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     8761 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     8821 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     8881 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     8941 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     9001 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     9061 tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     9121 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     9181 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     9241 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     9301 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     9361 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     9421 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     9481 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     9541 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     9601 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     9661 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     9721 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9781 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9841 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9901 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9961 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag
    10021 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    10081 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    10141 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    10201 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
    10261 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    10321 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    10381 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    10441 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    10501 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    10561 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    10621 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    10681 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    10741 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    10801 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    10861 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    10921 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    10981 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    11041 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    11101 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    11161 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    11221 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    11281 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    11341 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    11401 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    11461 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    11521 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
    11581 tctcccgatc ccctatggtc gactctcagt acaatctgct ctgatgccgc atagttaagc
    11641 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
    11701 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag g
//
