LOCUS V039022 8146 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V039022 VERSION V039022 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 744..770 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 774..800 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 804..830 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 837..2819 /label="WDR26(NM_025160)" /note="WDR26(NM_025160)" /gene="WDR26" misc_feature 2922..3510 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 3576..3624 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 3826..4254 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4268..4597 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4664..5458 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5634..5755 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5804..5820) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5828..5844 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5852..5882) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5897..5918 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6206..6794) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6965..7825) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7826..7930) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg 721 actctagagc tagcgccacc atgtacccat acgacgtacc tgattatgca acttatcctt 781 atgatgtacc tgattatgct agctatcctt atgatgtccc agactacgca aagcttatgc 841 aggccaatgg ggcaggagga ggaggaggag gtggcggcgg cggcggtgga ggaggagggg 901 gcggcggggg ccagggacag accccggaac tcgcctgctt gtcggcccag aacggggagt 961 cgtccccctc gtcgtcgtcg tccgcggggg acctggccca cgccaatggg ctcctgcctt 1021 ccgccccctc cgccgccagc aacaatagca acagcctgaa tgtcaataac ggggttcccg 1081 gcggggcggc cgccgcatcc tcagccaccg tcgcagctgc ctccgccacc accgccgcct 1141 cctcttcctt ggccacccca gaactgggca gcagcctcaa gaagaagaag cggctctccc 1201 agtcagatga ggatgtcatt aggctaatag gacagcactt gaatggctta gggctcaacc 1261 agactgttga tctcctcatg caagagtcag gatgtcgttt agaacatcct tctgctacca 1321 aattccgaaa tcatgtcatg gaaggagact gggataaggc agaaaatgac ctgaatgaac 1381 taaagccttt agtgcattct cctcatgcta ttgtggtaag aggcgcactt gaaatctctc 1441 aaacgttgtt gggaataatt gtgaggatga agtttttgct gctgcagcag aagtacctag 1501 aatacctgga ggatggcaag gtcctggagg cacttcaagt tctacgctgt gaattgacgc 1561 cgctgaaata caatacagag cgcattcatg ttcttagtgg gtatctgatg tgtagccatg 1621 cagaagacct acgtgcaaaa gcagaatggg aaggcaaagg gacagcttcc cgatctaaac 1681 tattggataa acttcagacc tatttaccac catcagtgat gcttccccca cggcgtttac 1741 agactctcct gcggcaggcg gtggaactac aaagggatcg gtgcctatat cacaatacca 1801 aacttgataa taatctagat tctgtgtctc tgcttataga ccatgtttgt agtaggaggc 1861 agttcccatg ttatacgcag cagatactta cggagcattg taatgaagtg tggttctgta 1921 aattctctaa tgatggcact aaactagcaa caggatcaaa agatacaaca gttatcatat 1981 ggcaagttga tccggataca cacctgctaa aactgcttaa aacattagaa ggacatgctt 2041 atggcgtttc ttatattgca tggagtccag atgacaacta tcttgttgct tgtggcccag 2101 atgactgctc tgagctttgg ctttggaatg tacaaacagg agaactaagg acaaaaatga 2161 gccagtctca tgaagacagt ttgacaagtg tggcttggaa tccagatggg aagcgctttg 2221 tgactggagg tcagcgtggg cagttctatc agtgtgactt agatggtaat ctccttgact 2281 cctgggaagg ggtaagagtg caatgccttt ggtgcttgag tgatggaaag actgttctgg 2341 catcagatac acaccagcga attcggggct ataacttcga ggaccttaca gataggaaca 2401 tagtacaaga agatcatcct attatgtctt ttactatttc aaaaaatggc cgattagctt 2461 tgttaaatgt agcaactcag ggagttcatt tatgggactt gcaagacaga gttttagtaa 2521 gaaagtatca aggtgttaca caagggtttt atacaattca ttcatgtttt ggaggccata 2581 atgaagactt catcgctagt ggcagtgaag atcacaaggt ttacatctgg cacaaacgta 2641 gtgaactgcc aattgcggag ctgacagggc acacacgtac agtaaactgt gtgagctgga 2701 acccacagat tccatccatg atggccagcg cctcagatga tggcactgtt agaatatggg 2761 gaccagcacc ttttatagac caccagaata ttgaagagga atgcagtagc atggatagtt 2821 gagaattctg cagatatcca gcacagtggc ggccgctcga gtctagaggg ccccttaagg 2881 gttcgatccc taccggttag taatgagttt gatatctcga caatcaacct ctggattaca 2941 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 3001 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 3061 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 3121 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 3181 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 3241 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 3301 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga 3361 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 3421 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 3481 gtcggatctc cctttgggcc gcctccccgc ctggaaacgg gggaggctaa ctgaaacacg 3541 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac 3601 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg 3661 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac 3721 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc 3781 ctgccatagc agatctgcgc agctggggct ctagggggta tccccacgcg ccctgtagcg 3841 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 3901 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 3961 cccgtcaagc tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc 4021 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 4081 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 4141 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgggga 4201 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct 4261 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 4321 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 4381 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 4441 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 4501 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta 4561 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc 4621 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 4681 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 4741 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 4801 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg 4861 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 4921 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 4981 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 5041 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 5101 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 5161 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt 5221 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 5281 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 5341 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 5401 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 5461 gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt 5521 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg 5581 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt 5641 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 5701 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 5761 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 5821 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 5881 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 5941 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 6001 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 6061 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 6121 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 6181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 6241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 6301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 6361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc 6421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 6481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 6541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 6601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 6661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 6721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 6781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 6841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 6901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 6961 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 7021 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 7081 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 7141 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 7201 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 7261 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 7321 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 7381 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 7441 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 7501 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 7561 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 7621 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 7681 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 7741 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 7801 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 7861 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 7921 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc 7981 cgatccccta tggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta 8041 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac 8101 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagg //