LOCUS       V031004                 5742 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031004
VERSION     V031004
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..1889
                     /label="Vav3(NM_146139)"
                     /note="Vav3(NM_146139)"
                     /gene="Vav3"
     CDS             1908..1937
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             1956..1979
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(1999..2015)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2026..2648
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2797..3385)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3714..3761
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(3993..4787)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4822..5179)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5181..5285)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5312..5740
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gccaattttt acctttgttt cagaacaagg gccattcaaa ccaccagaga
     1081 aacggaccaa tggacttcgg agagcttcca gacaggtgga cccaggtttg ccgaagatgc
     1141 aggtcattag gaactacact ggcaccccgg ccccagggct tcatgaagga ccaccattgc
     1201 atatccaggc aggcgacact gttgaacttc tgagaggaga tgcacacagt gtgttctggc
     1261 agggacgaaa cttagcttca ggagaggttg gatttttccc aagtgatgct gtcaaaccct
     1321 ctccatgtgt acctaaacca gtagattatt cttgccaacc ctggtatgct ggcccaatgg
     1381 aaagattgca ggcagaaact gaacttatta acagggtgaa tagtacttac cttgtaaggc
     1441 acaggaccaa agaatctgga gaatatgcaa ttagcattaa gtacaataat gaagcaaaac
     1501 acatcaagat tttaacaaga gatggctttt ttcacattgc agaaaataga aaatttaaaa
     1561 gcttaatgga acttgtggag tactacaagc accattctct caaagaaggg ttcaggacct
     1621 tagatacaac gctacagttt ccatacaagg agccagaaca gccagctgga cagaggggta
     1681 atagaacagg caacagcttg ttgagtccca aagtactggg tattgccatc gctcggtacg
     1741 acttctgtgc aagagatatg cgggagctct ccctattgaa aggagacatg gtgaagattt
     1801 acacgaagat gagtgcaaat ggctggtgga gaggtgaagt aaatggcagg gtgggctggt
     1861 ttccatccac atatgtggaa gaagatgaaa cgcgtacgcg gccgctcgag cagaaactca
     1921 tctcagaaga ggatctggca gcaaatgata tcctggatta caaggatgac gacgataagg
     1981 tttaaacggc cggccgcggt catagctgtt tcctgaacag atcccgggtg gcatccctgt
     2041 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
     2101 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
     2161 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
     2221 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc
     2281 gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca
     2341 tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg
     2401 gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg
     2461 ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt aaaataacta
     2521 taccagcagg aggacgtcca gacacagcat aggctacctg gccatgccca accggtggga
     2581 catttgagtt gcttgcttgg cactgtcctc tcatgcgttg ggtccactca gtagatgcct
     2641 gttgaattgg gtacgcggcc agcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2701 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2761 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2821 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2881 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2941 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3001 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3061 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3121 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3181 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3241 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3301 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3361 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3421 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3481 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3541 acctgaggct atggcagggc ctgccgcccc gacgttggct gcgagccctg ggccttcacc
     3601 cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc cccaatgggg
     3661 tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa
     3721 cgacccaaca ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc
     3781 cggtattgtc tccttccgtg tttcagttag cctcccccta gggtgggcga agaactccag
     3841 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
     3901 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc
     3961 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
     4021 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     4081 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcga
     4141 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     4201 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgctc
     4261 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     4321 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
     4381 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
     4441 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
     4501 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
     4561 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg
     4621 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
     4681 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
     4741 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
     4801 catcctgtct cttgatcgat ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
     4861 cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag
     4921 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     4981 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     5041 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
     5101 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cattccacag
     5161 ctggttcttt ccgcctcagg actcttcctt tttcaatatt attgaagcat ttatcagggt
     5221 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     5281 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     5341 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5401 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5461 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5521 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5581 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5641 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5701 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt tt
//
