LOCUS       V031057                 7386 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031057
VERSION     V031057
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3533
                     /label="Usp26(NM_031388)"
                     /note="Usp26(NM_031388)"
                     /gene="Usp26"
     CDS             3552..3581
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3600..3623
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3643..3659)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3670..4292
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4441..5029)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5358..5405
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5637..6431)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6466..6823)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6825..6929)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6956..7384
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggagcccata ttgataaatg ctcaagtcca gatgtggagt gcaaaggcag
     1081 gaatgtccaa gtcaagaaat gcactcattg aaacatgtgt aggaaaacga gaagttaaac
     1141 ttattctcta tttcagcact ggaaagatta agactttgca actacacgat aatattaaaa
     1201 gtgtggtcct tcaaacctat ggcgaagacc agaattacct acatttgact tttaaaaata
     1261 atgatttctt gtttgttgag aaactcacca ccacagatgc cagaagactg aagagatttc
     1321 tagacaaaac ctctcaaggt agtattcggc cagccagaag tgatgagaga tgtggtgagc
     1381 ctagcacaag tgcacaggag ttgaatggct ctggaagttc atgtgaaaca aatagtgagt
     1441 gctttgaatc acccaaagaa agtgaaatgt gcatgtttcg tgagttgtct ttgcttccat
     1501 cctcatcaac ctttcttcac aatgtaggat tattagaaaa ccaattcata aagaggaaaa
     1561 gatttttctc tgatttagca aaaaatgaaa aacagagcaa cctgaaggac agtatcaggg
     1621 actttgaggc aaatttagtg gtgtgtatct ctaatgaaaa gggaaaagaa aggaatgtaa
     1681 gagaagtaga catcagtaag ccagggtttg gatttccatt tgagaccaac tatcctgaag
     1741 atagtggtgt ggatgttcgt gatcttaatg atctcattac aaaattattt tctccagttc
     1801 tgttggaaac acactgtatt gagaacggcc tagagtggca tgaatatatg aagacatact
     1861 tgctttaccc agagaaattg tggcaaggcc tgcctaatgt gggaaacacc tgctatataa
     1921 atgttgtatt acagtctcta tgctcaatac cactgtttat taatgattta ttcaaccagg
     1981 gtttcccatg gattaaagct cccaaagatg attttaacat gctcttgatg caactgcttg
     2041 ttttgaaaga tatttacaac gcaagattta gacagaagtt acttataggt attacaaaag
     2101 ccctccccat atttggagag atatttgctg ttgacaggca gaatgatgct catgagtttt
     2161 taagtctctg tttagttcag ttgaaggaga ctttccaaag agtaaccatg atgtggcagt
     2221 ctgaaaatga ttcgggggat ttttacttac ttaaagacat ttttgctgat tatgctacta
     2281 tcaacaaaat gcccgtttgt cctgttacca ataattttga atttgagttg ctaagctcca
     2341 ttttttgtaa agcttgtggc ctgactcttt ttaagggaga accaagtaga tacctttcta
     2401 tcaacattcc ccaaggaggg aaagacatgt ccatccagtc cactttagat cttttcttta
     2461 gtgcagagga gcttgagcat aggtgtgaaa agtgtttgta caacaaatct gtttcatttc
     2521 acaggtttgg ccggctaccc agggtaatta ttgttcatct gaaacgctat cactttaatg
     2581 agtcatgggt aatgaagaag gatgagcggc ccatccttgt ttccaaatac ttaaggctgt
     2641 cttgtcactg tagcaaaagc acaaaaccgc ccccacccct tcgcccaggt gaacatgtta
     2701 agaatcttga cttattaaaa ccccttgaag tgttgggttc cgaaatactc aaattgcctt
     2761 ttaattcagt gaggacctct agatccaagg gtttcgaaac tataaacatc acatcaaaca
     2821 gggagtcaga agcacaaagt gggaaaagag tctctgaagt gttgagtgga aaagtgcagc
     2881 aggaaaattc agggaaaggt gacacagcac atatagttgg gtcagaactt acaaaggaga
     2941 ctgagaaact caagaaacat gaggaagagc atagacccag tgatttagat tctggtagta
     3001 tcagggaggc ccaaaagtac caacaggctg agaaatgtaa cgaagggaga agtgataagc
     3061 agatttccct agaggcactt actcaaagcc gtccaaaacc aatctcccag gaacagacag
     3121 aaaaccttgg gaaaactaca ctgtcacata cccaggatag tagtcagagt tcacagagct
     3181 catcagattc cagtaagagc tcccgatgca gtgatgatct cgataagaag gcaaagccta
     3241 cacgcaaggt ggatccaaca aagttaaata aaaaagaaga taatgtttac aggcttgtta
     3301 atattatcaa ccatattggg aacagtccca atggaggcca ctacatcaat gatgcctttg
     3361 acttcaagag gcagagttgg ttcacttata gtgatctaca tgtaacaaga acccaagagg
     3421 actttgtata taggggtcgg agttctactg ggtatgtctt cttttacatg cataatgata
     3481 tatttgaaga gctcttggca aaggaaactc agtctaccag cacatccaag ggtacgcgta
     3541 cgcggccgct cgagcagaaa ctcatctcag aagaggatct ggcagcaaat gatatcctgg
     3601 attacaagga tgacgacgat aaggtttaaa cggccggccg cggtcatagc tgtttcctga
     3661 acagatcccg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt
     3721 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
     3781 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
     3841 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
     3901 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct
     3961 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg
     4021 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc
     4081 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
     4141 gtccttctga ttttaaaata actataccag caggaggacg tccagacaca gcataggcta
     4201 cctggccatg cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc
     4261 gttgggtcca ctcagtagat gcctgttgaa ttgggtacgc ggccagcggc gagcggtatc
     4321 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4381 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4441 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4501 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4561 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4621 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4681 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4741 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4801 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4861 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4921 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4981 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5041 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5101 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5161 atcaatctaa agtatatatg agtaacctga ggctatggca gggcctgccg ccccgacgtt
     5221 ggctgcgagc cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac
     5281 gcgggcgtat tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga
     5341 ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat
     5401 tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc
     5461 cctagggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc
     5521 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc
     5581 tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag
     5641 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     5701 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     5761 gccaacgcta tgtcctgata gcgatccgcc acacccagcc ggccacagtc gatgaatcca
     5821 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
     5881 agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc
     5941 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     6001 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     6061 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     6121 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     6181 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     6241 gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     6301 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     6361 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     6421 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cgatctttgc aaaagcctag
     6481 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
     6541 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     6601 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg
     6661 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga
     6721 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     6781 ccctaactga cacacattcc acagctggtt ctttccgcct caggactctt cctttttcaa
     6841 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     6901 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
     6961 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     7021 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     7081 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     7141 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     7201 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     7261 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     7321 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     7381 aatttt
//
