LOCUS       V025502                 8428 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025502
VERSION     V025502
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..3084
                     /label="UHRF1(NM_001048201)"
                     /note="UHRF1(NM_001048201)"
                     /gene="UHRF1"
     CDS             3091..3180
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     misc_feature    3204..3792
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3858..3906
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4108..4536
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4550..4879
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4946..5740
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5916..6037
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6086..6102)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6110..6126
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6134..6164)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6179..6200
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6488..7076)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7247..8107)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8108..8212)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgtg gatccaggtt
      721 cggaccatgg acgggaggca gacccacacg gtggactcgc tgtccaggct gaccaaggtg
      781 gaggagctga ggcggaagat ccaggagctg ttccacgtgg agccaggcct gcagaggctg
      841 ttctacaggg gcaaacagat ggaggacggc cataccctct tcgactacga ggtccgcctg
      901 aatgacacca tccagctcct ggtccgccag agcctcgtgc tcccccacag caccaaggag
      961 cgggactccg agctctccga caccgactcc ggctgctgcc tgggccagag tgagtcagac
     1021 aagtcctcca cccacggtga ggcggccgcc gagactgaca gcaggccagc cgatgaggac
     1081 atgtgggatg agacggaatt ggggctgtac aaggtcaatg agtacgtcga tgctcgggac
     1141 acgaacatgg gggcgtggtt tgaggcgcag gtggtcaggg tgacgcggaa ggccccctcc
     1201 cgggacgagc cctgcagctc cacgtccagg ccggcgctgg aggaggacgt catttaccac
     1261 gtgaaatacg acgactaccc ggagaacggc gtggtccaga tgaactccag ggacgtccga
     1321 gcgcgcgccc gcaccatcat caagtggcag gacctggagg tgggccaggt ggtcatgctc
     1381 aactacaacc ccgacaaccc caaggagcgg ggcttctggt acgacgcgga gatctccagg
     1441 aagcgcgaga ccaggacggc gcgggaactc tacgccaacg tggtgctggg ggatgattct
     1501 ctgaacgact gtcggatcat cttcgtggac gaagtcttca agattgagcg gccgggtgaa
     1561 gggagcccca tggttgacaa ccccatgaga cggaagagcg ggccgtcctg caagcactgc
     1621 aaggacgacg tgaacagact ctgccgggtc tgcgcctgcc acctgtgcgg gggccggcag
     1681 gaccccgaca agcagctcat gtgcgatgag tgcgacatgg ccttccacat ctactgcctg
     1741 gacccgcccc tcagcagtgt tcccagcgag gacgagtggt actgccctga gtgccggaat
     1801 gatgccagcg aggtggtact ggcgggagag cggctgagag agagcaagaa gaaggcgaag
     1861 atggcctcgg ccacatcgtc ctcacagcgg gactggggca agggcatggc ctgtgtgggc
     1921 cgcaccaagg aatgtaccat cgtcccgtcc aaccactacg gacccatccc ggggatcccc
     1981 gtgggcacca tgtggcggtt ccgagtccag gtcagcgagt cgggtgtcca tcggccccac
     2041 gtggctggca tacacggccg gagcaacgac ggagcgtact ccctagtcct ggcggggggc
     2101 tatgaggatg atgtggacca tgggaatttt ttcacataca cgggtagtgg tggtcgagat
     2161 ctttccggca acaagaggac cgcggaacag tcttgtgatc agaaactcac caacaccaac
     2221 agggcgctgg ctctcaactg ctttgctccc atcaatgacc aagaaggggc cgaggccaag
     2281 gactggcggt cggggaagcc ggtcagggtg gtgcgcaatg tcaagggtgg caagaatagc
     2341 aagtacgccc ccgctgaggg caaccgctac gatggcatct acaaggttgt gaaatactgg
     2401 cccgagaagg ggaagtccgg gtttctcgtg tggcgctacc ttctgcggag ggacgatgat
     2461 gagcctggcc cttggacgaa ggaggggaag gaccggatca agaagctggg gctgaccatg
     2521 cagtatccag aaggctacct ggaagccctg gccaaccgag agcgagagaa ggagaacagc
     2581 aagagggagg aggaggagca gcaggagggg ggcttcgcgt cccccaggac gggcaagggc
     2641 aagtggaagc ggaagtcggc aggaggtggc ccgagcaggg ccgggtcccc gcgccggaca
     2701 tccaagaaaa ccaaggtgga gccctacagt ctcacggccc agcagagcag cctcatcaga
     2761 gaggacaaga gcaacgccaa gctgtggaat gaggtcctgg cgtcactcaa ggaccggccg
     2821 gcgagcggca gcccgttcca gttgttcctg agtaaagtgg aggagacgtt ccagtgtatc
     2881 tgctgtcagg agctggtgtt ccggcccatc acgaccgtgt gccagcacaa cgtgtgcaag
     2941 gactgcctgg acagatcctt tcgggcacag gtgttcagct gccctgcctg ccgctacgac
     3001 ctgggccgca gctatgccat gcaggtgaac cagcctctgc agaccgtcct caaccagctc
     3061 ttccccggct acggcaatgg ccggctcgag gaacaaaaac taatatcaga ggaagatttg
     3121 gaacagaaac taatcagtga agaagattta gagcaaaagt taatttccga ggaggactta
     3181 tagtgagggc ccgatatctc gacaatcaac ctctggatta caaaatttgt gaaagattga
     3241 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     3301 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     3361 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     3421 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     3481 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     3541 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     3601 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     3661 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     3721 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     3781 ccgcctcccc gcctggaaac gggggaggct aactgaaaca cggaaggaga caataccgga
     3841 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt
     3901 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     3961 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     4021 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcagatctgc
     4081 gcagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     4141 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4201 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4261 ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4321 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4381 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     4441 ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc tattggttaa
     4501 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     4561 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4621 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4681 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4741 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4801 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     4861 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     4921 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     4981 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     5041 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     5101 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     5161 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     5221 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     5281 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     5341 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     5401 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     5461 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     5521 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     5581 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     5641 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     5701 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgcg
     5761 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     5821 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     5881 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     5941 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6001 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     6061 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6121 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6181 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6241 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6301 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6361 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6421 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6481 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6541 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     6601 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     6661 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     6721 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     6781 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6841 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6901 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     6961 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7021 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7081 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7141 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7201 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7261 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7321 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7381 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7441 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7501 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     7561 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     7621 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     7681 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     7741 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     7801 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     7861 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     7921 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     7981 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8041 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8101 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8161 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8221 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtcgac
     8281 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     8341 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     8401 cgacaattgc atgaagaatc tgcttagg
//
