LOCUS       V030985                 5616 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030985
VERSION     V030985
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..1763
                     /label="Tshr(NM_001113404)"
                     /note="Tshr(NM_001113404)"
                     /gene="Tshr"
     CDS             1782..1811
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             1830..1853
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(1873..1889)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    1900..2522
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2671..3259)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3588..3635
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(3867..4661)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4696..5053)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5055..5159)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5186..5614
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gaggccaggg tccctgctgc tgcttgttct gctgctcgcc ctgtccagga
     1081 gcctgcgggg caaagagtgt gcgtctccac cctgtgagtg tcaccaggag gacgacttca
     1141 gagtcacctg caaggagctc caccgaatcc ccagcctgcc gcccagcacc cagactctga
     1201 agctcatcga gactcatctg aagaccatac ccagtcttgc attttcgagt ctgcccaata
     1261 tttccaggat ctatttatct atagatgcaa ctctgcagcg gctggaacca cattctttct
     1321 acaatttgag taaaatgact cacatagaaa tccggaacac cagaagctta acctatatag
     1381 accctgatgc cttgacagag ctccccttgc tcaagtttct tggcattttc aatactggac
     1441 ttagaatatt ccctgacttg accaaaattt attccacgga catattcttt atacttgaaa
     1501 tcacagacaa cccttacatg acttcggtcc ctgaaaacgc attccagggc ctatgcaatg
     1561 aaaccttgac cctgaaactg tacaacaatg gatttacttc agtccaagga catgctttca
     1621 atggaacaaa gctggatgct gtttacctaa acaagaataa atacctgaca gctatagaca
     1681 acgatgcctt tggaggagta tacagtggac caactttgct gtcatttctc aaagaagaat
     1741 ttgatttcca ccaggcacaa atcacgcgta cgcggccgct cgagcagaaa ctcatctcag
     1801 aagaggatct ggcagcaaat gatatcctgg attacaagga tgacgacgat aaggtttaaa
     1861 cggccggccg cggtcatagc tgtttcctga acagatcccg ggtggcatcc ctgtgacccc
     1921 tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta
     1981 ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg
     2041 aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc
     2101 tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc
     2161 tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg
     2221 accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg
     2281 ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga
     2341 ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttaaaata actataccag
     2401 caggaggacg tccagacaca gcataggcta cctggccatg cccaaccggt gggacatttg
     2461 agttgcttgc ttggcactgt cctctcatgc gttgggtcca ctcagtagat gcctgttgaa
     2521 ttgggtacgc ggccagcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     2581 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     2641 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2701 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2761 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2821 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2881 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2941 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3001 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3061 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3121 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3181 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3241 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3301 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3361 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaacctga
     3421 ggctatggca gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact
     3481 tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat tggccccaat ggggtctcgg
     3541 tggggtatcg acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc
     3601 aacaccgtgc gttttattct gtctttttat tgccgtcata gcgcgggttc cttccggtat
     3661 tgtctccttc cgtgtttcag ttagcctccc cctagggtgg gcgaagaact ccagcatgag
     3721 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     3781 ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc
     3841 ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg
     3901 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     3961 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcgatccgcc
     4021 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     4081 ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg
     4141 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     4201 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     4261 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     4321 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     4381 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     4441 cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg
     4501 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     4561 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     4621 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct
     4681 gtctcttgat cgatctttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     4741 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     4801 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     4861 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     4921 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     4981 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt
     5041 ctttccgcct caggactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5101 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     5161 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5221 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     5281 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5341 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5401 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5461 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     5521 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     5581 aatgagctga tttaacaaaa atttaacgcg aatttt
//
