LOCUS       V031040                 6456 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031040
VERSION     V031040
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2603
                     /label="Tsen54(NM_029557)"
                     /note="Tsen54(NM_029557)"
                     /gene="Tsen54"
     CDS             2622..2651
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2670..2693
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2713..2729)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2740..3362
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3511..4099)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4428..4475
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4707..5501)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5536..5893)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5895..5999)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6026..6454
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggagcccgaa cccgaacccg ggtcggtgga ggttcccgcg gggcgtgtgc
     1081 tcagcgcctc ggagctccgc gccgcgcgct cgcggtccca gaagctacca cagcggtcgc
     1141 atggccccaa ggacttcctc ccggacggct cggaggccca ggcggagcgg ctgcgcctgt
     1201 gtcgccaaga gctgtggcag ctgctggcgg aggagcgcgt ggagcgcctg ggcagcttgg
     1261 tggccgcgga gtggaaacca gaagagggct ttgtggagtt gacgtctcct gcggggaaat
     1321 tctggcagac catgggctac tcagaggaag gacggcagcg acttcacccc gaagaggctt
     1381 tgtatctgct ggagtgtggt tcgattcagc tcttctacca agacctccca ctgtctatcc
     1441 aagaggcata ccagctgctg ctgacagagg acaccttgag tttcctgcag taccaggtct
     1501 tcagccacct gaagagactg ggctatgtgg ttcgtcggtt ccagctaagc tctgtcgtgt
     1561 caccttacga gcggcagctt aatttggatg gttatgccca gtgcctggag gatgggtctg
     1621 gcaagaggaa gaggagttcc agctgtcggt ctgttaataa gaagcccaag gtcctgcaga
     1681 actccctgcc gcccgtcagc ctggcagcct ccagctcacc tgcctgtgac cagagtagcc
     1741 aatacccaga ggagaagtct caggactcaa gccccaggca gggctcagag ctccccttgc
     1801 agttcctggg gtcctcagag ccttgctctg atctggccag ggaagacgtg gggtgtgacc
     1861 gagagagtca caaaatagag aatggagcta agggaacccc taagctacgc tggaactttg
     1921 agcagatctc attccccaac atggcctctg atagtcgcca cactttcctg cctgctccag
     1981 ccccagagct gctcccagcc aatgtcattg ggcgagggac agatgctgag tcctggtgcc
     2041 aaaagctgaa ccagcggagg gagaagctct cccgtagaga ccgggaacag caagcagtgg
     2101 tccagcagtt ccgggaggat gttaacgcag atcccgaggt gcggggctgc tccagctggc
     2161 aggagtacaa ggagctgctg cagaggcgac agacgcagaa gagccagccc cggcctccac
     2221 acctatgggg ccagtccgtt acccccttgc tcgaccctga taaagccgat tgtccagccg
     2281 cggtcctgca acatatctct gtactgcaga cgacacacct tgctgatgga ggttaccggc
     2341 tgctggagaa gtctggaggc ttgcagatca gcttcgatgt ttaccaggct gacgctgtgg
     2401 cgacgttccg aaaaaacagc cctggcaaac cctatgtgcg aatgtgcatt agtgggtttg
     2461 atgaccctgt cccagacctc tgcagcctca agtgcttgac ctaccagagt ggggatgttc
     2521 ccctgatctt tgctctagtg gaccacggtg acatctcctt ctacagcttc agagacttca
     2581 cactgcccag ggatctgggc cacacgcgta cgcggccgct cgagcagaaa ctcatctcag
     2641 aagaggatct ggcagcaaat gatatcctgg attacaagga tgacgacgat aaggtttaaa
     2701 cggccggccg cggtcatagc tgtttcctga acagatcccg ggtggcatcc ctgtgacccc
     2761 tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta
     2821 ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg
     2881 aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc
     2941 tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc
     3001 tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg
     3061 accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg
     3121 ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga
     3181 ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttaaaata actataccag
     3241 caggaggacg tccagacaca gcataggcta cctggccatg cccaaccggt gggacatttg
     3301 agttgcttgc ttggcactgt cctctcatgc gttgggtcca ctcagtagat gcctgttgaa
     3361 ttgggtacgc ggccagcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3421 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3481 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3541 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3601 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3661 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3721 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3781 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3841 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3901 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3961 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4021 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     4081 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     4141 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     4201 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaacctga
     4261 ggctatggca gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact
     4321 tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat tggccccaat ggggtctcgg
     4381 tggggtatcg acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc
     4441 aacaccgtgc gttttattct gtctttttat tgccgtcata gcgcgggttc cttccggtat
     4501 tgtctccttc cgtgtttcag ttagcctccc cctagggtgg gcgaagaact ccagcatgag
     4561 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     4621 ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc
     4681 ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg
     4741 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     4801 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcgatccgcc
     4861 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     4921 ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg
     4981 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     5041 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     5101 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     5161 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     5221 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     5281 cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg
     5341 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     5401 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     5461 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct
     5521 gtctcttgat cgatctttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     5581 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     5641 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     5701 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     5761 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     5821 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt
     5881 ctttccgcct caggactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5941 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6001 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
     6061 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     6121 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     6181 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     6241 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     6301 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6361 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6421 aatgagctga tttaacaaaa atttaacgcg aatttt
//
