LOCUS       V031052                 7398 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031052
VERSION     V031052
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3545
                     /label="Trpv1(NM_001001445)"
                     /note="Trpv1(NM_001001445)"
                     /gene="Trpv1"
     CDS             3564..3593
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3612..3635
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3655..3671)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3682..4304
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4453..5041)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5370..5417
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5649..6443)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6478..6835)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6837..6941)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6968..7396
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggagaaatgg gctagcttag actcggatga atctgagccc ccagcccaag
     1081 agaactcctg cccggaccct ccagacagag accctaactc caagccgcct ccagccaagc
     1141 cccacatctt tgctaccagg agtcgcaccc ggctttttgg gaagggtgac tcagaagagg
     1201 cctctcccat ggactgccct tatgaggaag gcgggctggc ctcctgccct atcatcaccg
     1261 tcagctctgt tgtcactctc cagaggtctg tggatggacc tacctgtctc aggcagacat
     1321 cccaggactc tgtctccact ggtgttgaga cgcccccaag gctctatgat cgcaggagca
     1381 tcttcgacgc tgtggctcag agcaactgcc aggagctgga gagcctgctg tccttcctgc
     1441 agaagagcaa gaagcgcctg actgacagcg agttcaaaga cccagagacg ggaaagacct
     1501 gtctgctcaa agccatgctc aatctgcaca atgggcagaa cgacaccatt gctctgctcc
     1561 tggacattgc ccggaagaca gatagcctga agcagtttgt caatgccagc tacacagaca
     1621 gctactacaa gggccagaca gcattacaca ttgccattga aaggcggaac atggcactgg
     1681 tgaccctctt ggtggagaat ggagcagatg tccaggctgc tgctaacggg gacttcttca
     1741 agaaaaccaa agggaggcct ggcttctact ttggtgagct gcccctgtcc ctggctgcgt
     1801 gcaccaacca gctggccatt gtgaagttcc tgctgcagaa ctcctggcag cctgcagaca
     1861 tcagtgcacg ggattcggtg ggcaacacgg tgctgcacgc ccttgtggag gtggcagata
     1921 acacagctga caacaccaag ttcgtgacaa acatgtacaa cgagatcctg atcctggggg
     1981 ccaaactcca ccccacactg aagctagaag aactcaccaa caagaagggg cttacaccgc
     2041 tggctctggc tgccagcagt gggaagattg gggtcttggc ctacattctc cagagggaga
     2101 tccacgaacc agagtgccgg cacctgtcca ggaagttcac tgaatgggcc tatgggcccg
     2161 tgcactcctc cctttatgac ctgtcctgca ttgacacctg tgagaagaat tcagtgctgg
     2221 aggtgatcgc ctacagtagc agtgagaccc ccaaccgcca cgacatgctt ctcgtggagc
     2281 ccttgaaccg actcctgcag gacaagtggg acagatttgt caagcgcatc ttctacttca
     2341 acttcttcgt ctactgcttg tatatgatca tcttcaccac ggctgcttac tatcggcctg
     2401 tggaaggctt gcccccctat aagctgaata acaccgttgg ggactatttc cgtgtcactg
     2461 gagagatcct gtctgtgtca ggaggagtct acttcttctt ccgagggatc cagtatttcc
     2521 tgcagaggcg accatccctc aagagtttgt ttgtggacag ctacagtgag atacttttct
     2581 ttgtacagtc actgttcatg ctggtgtctg tggtactgta cttcagccat cgcaaggagt
     2641 atgtggcttc catggtgttc tccctggcca tgggctggac caacatgctc tactacaccc
     2701 gaggattcca gcagatgggc atctatgctg tcatgattga gaagatgatc ctcagagacc
     2761 tgtgtcggtt tatgttcgtc tacctcgtgt tcttgtttgg attttccaca gccgtagtga
     2821 cactgatcga ggatgggaag aataactcac tgcctgtgga gtccccacca cacaagtgtc
     2881 ggggatctgc ctgcaggcca ggtaactctt acaacagcct gtattccaca tgtctggagc
     2941 tgttcaagtt caccatcggc atgggtgacc tggagttcac cgagaactat gacttcaagg
     3001 ctgtcttcat catcctgtta ctggcctatg tgattctcac ctacatcctc ctgctcaaca
     3061 tgctcattgc tctcatgggc gagactgtca acaagattgc acaagagagc aagaacatct
     3121 ggaagctgca gcgagccatc accatcctgg atacagagaa gagtttcctg aagtgcatga
     3181 ggaaggcctt ccgctccggc aagctgctgc aggtggggtt cacgccggac ggcaaggatg
     3241 acttccggtg gtgcttcagg gtggatgagg tgaactggac tacctggaac accaacgtgg
     3301 gcatcatcaa cgaggaccca ggcaactgtg agggcgtcaa gcgcaccctg agcttctccc
     3361 tgcggtcagg ccgagtttca gggagaaact ggaagaactt tgccctggtt ccccttctga
     3421 gggacgcaag cactcgagat aggcatagca cccagccgga agaagttcag ctgaagcact
     3481 atacgggatc ccttaagcca gaggatgctg aggtcttcaa ggattccatg gccccagggg
     3541 agaaaacgcg tacgcggccg ctcgagcaga aactcatctc agaagaggat ctggcagcaa
     3601 atgatatcct ggattacaag gatgacgacg ataaggttta aacggccggc cgcggtcata
     3661 gctgtttcct gaacagatcc cgggtggcat ccctgtgacc cctccccagt gcctctcctg
     3721 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
     3781 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
     3841 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg
     3901 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc
     3961 ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt
     4021 ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa
     4081 tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact
     4141 gctcccttcc ctgtccttct gattttaaaa taactatacc agcaggagga cgtccagaca
     4201 cagcataggc tacctggcca tgcccaaccg gtgggacatt tgagttgctt gcttggcact
     4261 gtcctctcat gcgttgggtc cactcagtag atgcctgttg aattgggtac gcggccagcg
     4321 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     4381 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     4441 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     4501 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     4561 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     4621 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     4681 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     4741 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     4801 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     4861 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     4921 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     4981 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     5041 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     5101 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     5161 atgaagtttt aaatcaatct aaagtatata tgagtaacct gaggctatgg cagggcctgc
     5221 cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttggggggt ggggtgggga
     5281 aaaggaagaa acgcgggcgt attggcccca atggggtctc ggtggggtat cgacagagtg
     5341 ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt gcgttttatt
     5401 ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc
     5461 agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc gctggaggat
     5521 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt
     5581 ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag
     5641 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
     5701 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
     5761 atatcacggg tagccaacgc tatgtcctga tagcgatccg ccacacccag ccggccacag
     5821 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
     5881 tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg
     5941 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
     6001 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
     6061 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
     6121 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
     6181 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
     6241 gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc ggtcttgaca
     6301 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
     6361 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
     6421 tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcgatcttt
     6481 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     6541 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     6601 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     6661 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     6721 cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     6781 ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc ctcaggactc
     6841 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6901 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6961 ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     7021 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     7081 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     7141 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     7201 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     7261 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     7321 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     7381 aaatttaacg cgaatttt
//
