LOCUS       V028097                 7684 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028097
VERSION     V028097
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..2340
                     /label="Trim26(NM_001025599)"
                     /note="Trim26(NM_001025599)"
                     /gene="Trim26"
     CDS             2347..2436
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     misc_feature    2460..3048
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3114..3162
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3364..3792
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3806..4135
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4202..4996
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5172..5293
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5342..5358)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5366..5382
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5390..5420)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5435..5456
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5744..6332)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6503..7363)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7364..7468)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc agtgtcagcc
      721 cccttgagga gcctggagga agaggtgacc tgctccatct gcctggatta cctgagggac
      781 ccggtgacca tcgactgtgg ccatgtcttc tgtcgcagct gcacaagtga tatccgcccc
      841 atatcgggga accggcctgt ctgcccgctc tgcaagaagc cctttaagaa ggagaacatc
      901 cgacccgtgt ggcagctagc aagtctggtg gagaacattg aacggctgaa ggtggacaat
      961 ggcagacagc caggagagct cgcccgcgag ccacaggaca tgaagttgtg tgagcggcac
     1021 caggagaagc tacactacta ctgtgaagat gatggcaaac tgttgtgtgt gatgtgcagg
     1081 gagtcccggg agcaccgccc ccacactgca gtcctggtgg agaaagccgc cctgcctcac
     1141 agagagaaaa ttctgaacca cttgaacacc ctaaggagag acagagacaa aatccagggc
     1201 tttcaagcca agggagaagc tgatattctg gctgcactga caaagctgca ggagcagagg
     1261 cagtacattg tggcggaatt taagcagggc caccagtttc taaagaagcg ggagcagcac
     1321 ctgctagacc agctggccac cttggagcag ctcctcaccg agggcaggga gaagttcaag
     1381 acccggggcg tcagtgagct tgaccggttg actctggtca tctccgagct ggagggcaag
     1441 gcacgacagc cagctgcaga gctgatgcag gatgtctgca ctacacagga caccaaggac
     1501 ttcgccaaca agtacccacg gaagaagttc tggattggga aagccatccc tcacatggtt
     1561 aaaagaaagg caggagaatt ctcagataaa cttctttctc tgcagcgagg cctgaggcag
     1621 ttccagggca agctgctgag agacttggag tataagacag taagcgtcac cctggaccca
     1681 cagtcggcca gtggatacct acatctttca gaggactgga agtgtgtgac ctatactggc
     1741 caataccaga gtgactgtct gctcccccag cagtttgact gtgagccagg agtgttgggc
     1801 agcaagggct tcacatgggg aaaggtatac tgggaagtag agttggagag agaaggctgg
     1861 tcagaggatg aagaagaggg ggaggaggaa gaagaagggg aagaagagga agaagacgag
     1921 gaggttggct atggggacgg atacgaagac tgggaaacag atgaagaaga cgaatcactg
     1981 ggggaagagg aagaggagga agaggaagaa gaagaagaag ttcaggaaag ctgcatggtg
     2041 ggagtggcca aagactctgt gaagaggaaa ggggacctct ccctgcgacc agaagatggt
     2101 gtgtgggccc ttcggctctc cccctcaggt atctgggcca acacgagccc agaggcccag
     2161 ctgttcccgg tgctgcggcc ccggagagtg ggcatcgccc tggattatga aggtggcact
     2221 gtgacattca ccaatgcaga gtcccaggaa ctcatctata ccttcacgac caccttcacc
     2281 cggcgcctgg ttcccttcct gtggctcaag tggccaggag cacgccttct gctgagaccc
     2341 ctcgaggaac aaaaactaat atcagaggaa gatttggaac agaaactaat cagtgaagaa
     2401 gatttagagc aaaagttaat ttccgaggag gacttatagt gagggcccga tatctcgaca
     2461 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     2521 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     2581 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     2641 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     2701 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     2761 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     2821 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     2881 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     2941 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3001 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaaacgggg
     3061 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     3121 aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     3181 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     3241 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     3301 acgtcggggc ggcaggccct gccatagcag atctgcgcag ctggggctct agggggtatc
     3361 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3421 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3481 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta gggttccgat
     3541 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     3601 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     3661 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     3721 tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     3781 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     3841 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     3901 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3961 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4021 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     4081 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     4141 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     4201 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4261 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4321 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     4381 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     4441 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     4501 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     4561 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     4621 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     4681 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     4741 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     4801 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     4861 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     4921 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     4981 tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca
     5041 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     5101 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     5161 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5221 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5281 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     5341 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     5401 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     5461 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     5521 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     5581 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     5641 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     5701 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     5761 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     5821 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     5881 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
     5941 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6001 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6061 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6121 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6181 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6241 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6301 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     6361 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     6421 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     6481 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     6541 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     6601 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     6661 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     6721 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     6781 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     6841 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     6901 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     6961 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7021 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7081 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     7141 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7201 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7261 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7321 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     7381 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     7441 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     7501 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc
     7561 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     7621 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     7681 tagg
//
