LOCUS       V024041                 5438 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024041
VERSION     V024041
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1055..1642
                     /label="Tmem126a(NM_025460)"
                     /note="Tmem126a(NM_025460)"
                     /gene="Tmem126a"
     CDS             1652..1675
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(1695..1711)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    1722..2344
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2493..3081)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3410..3457
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(3689..4483)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4518..4875)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(4877..4981)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5008..5436
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgcggc gcgccagatc tcaagcttgc caccatggaa agtcataaac caagtactag
     1081 caaagatgat ttaatactta atatcatatc cagaaaaatt aaacaacttc cagaatcaga
     1141 caggaatctg cttgaatatg gatcagcata tattggactt aatgctgctt ttgggggcct
     1201 aatagcaaac agtctatttc ggcgaatctt gaacgtgaca caagctcgat tagcttctag
     1261 cttaccaatg gctgtgatcc catttttgac agcaaacctg tcctaccaaa gtcttgtaag
     1321 tttacctttg agtacaggtg atttgaactg tgaaacctgt accacaacac ggggtgcact
     1381 agttggtttg gttatgggtg gtctgtaccc tatccttttg gctatacccg tcaatggggg
     1441 ccttgctgcc aggtatgaat caagtcccct cccacagagg ggaaacatct tcaactactg
     1501 gattacagtt tctaagcctg tctttagaaa aatgttattt cctactttac ttcagactgt
     1561 gtttgcatcc tatcttgggt ctagacaata taaactcctc ataaaggccc ttcagttacc
     1621 tgaacctgac ttagaaattc atgatatcct ggattacaag gatgacgacg ataaggttta
     1681 aacggccggc cgcggtcata gctgtttcct gaacagatcc cgggtggcat ccctgtgacc
     1741 cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc
     1801 taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt
     1861 ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg
     1921 tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct
     1981 cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca
     2041 tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca
     2101 ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg
     2161 gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa taactatacc
     2221 agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg gtgggacatt
     2281 tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag atgcctgttg
     2341 aattgggtac gcggccagcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2401 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2461 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2521 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2581 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2641 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2701 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2761 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2821 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2881 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     2941 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     3001 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3061 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3121 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3181 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaacct
     3241 gaggctatgg cagggcctgc cgccccgacg ttggctgcga gccctgggcc ttcacccgaa
     3301 cttggggggt ggggtgggga aaaggaagaa acgcgggcgt attggcccca atggggtctc
     3361 ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac
     3421 ccaacaccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt tccttccggt
     3481 attgtctcct tccgtgtttc agttagcctc cccctagggt gggcgaagaa ctccagcatg
     3541 agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac
     3601 ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg
     3661 tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg
     3721 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt
     3781 cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcgatccg
     3841 ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat
     3901 tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgctcgcct
     3961 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct
     4021 gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt
     4081 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
     4141 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc
     4201 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa
     4261 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac
     4321 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg
     4381 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc
     4441 aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc
     4501 ctgtctcttg atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc
     4561 tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag
     4621 ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg
     4681 ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     4741 gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt
     4801 ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgg
     4861 ttctttccgc ctcaggactc ttcctttttc aatattattg aagcatttat cagggttatt
     4921 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     4981 gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg
     5041 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5101 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5161 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5221 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5281 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5341 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5401 aaaatgagct gatttaacaa aaatttaacg cgaatttt
//
