LOCUS       V046834                 7399 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046834
VERSION     V046834
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..2058
                     /label="TUBA1A(NM_006009)"
                     /note="TUBA1A(NM_006009)"
                     /gene="TUBA1A"
     CDS             2065..2091
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2095..2121
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2125..2151
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    2175..2763
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    2829..2877
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3079..3507
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3521..3850
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3917..4711
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4887..5008
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5057..5073)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5081..5097
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5105..5135)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5150..5171
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5459..6047)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6218..7078)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7079..7183)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgcg tgagtgcatc
      721 tccatccacg ttggccaggc tggtgtccag attggcaatg cctgctggga gctctactgc
      781 ctggaacacg gcatccagcc cgatggccag atgccaagtg acaagaccat tgggggagga
      841 gatgattcct tcaacacctt cttcagtgag acgggggctg gcaagcatgt gccccgggca
      901 gtgtttgtag acttggaacc cacagtcatt gatgaagttc gcactggcac ctaccgccag
      961 ctcttccacc ctgagcaact tatcacaggc aaagaagatg ctgccaataa ctatgcccga
     1021 gggcactaca ccattggcaa ggagatcatt gacctcgtgt tggaccgaat tcgcaagctg
     1081 gccgaccagt gcacgggtct ccagggcttc ttggttttcc acagctttgg tgggggaact
     1141 ggttctgggt tcacctcgct gctcatggaa cgtctctcag ttgattatgg caagaagtcc
     1201 aagctggagt tctctattta cccggcgccc caggtttcca cagctgtagt tgagccctac
     1261 aactccatcc tcaccaccca caccaccctg gagcactctg attgtgcctt catggtagac
     1321 aatgaggcca tctatgacat ctgtcgtaga aacctcgata ttgagcgtcc aacctatact
     1381 aacctgaata ggttaatagg tcaaattgtg tcctccatca ctgcttccct gagatttgat
     1441 ggagccctga atgttgacct gacagaattc cagaccaacc tggtgcccta tccccgcatc
     1501 cacttccctc tggccacata tgcccctgtc atctctgctg agaaagccta ccatgaacag
     1561 ctttctgtag cagagatcac caatgcttgc tttgagccag ccaaccagat ggtgaaatgt
     1621 gaccctcgcc atggtaaata catggcttgc tgcctgttgt accgtggtga cgtggttccc
     1681 aaagatgtca atgctgccat tgccaccatc aagaccaagc gtaccatcca gtttgtggat
     1741 tggtgcccca ctggcttcaa ggttggcatc aactaccagc ctcccactgt ggtgcctggt
     1801 ggagacctgg ccaaggtaca gagagctgtg tgcatgctga gcaacaccac agccattgct
     1861 gaggcctggg ctcgcctgga ccacaagttt gacctgatgt atgccaaacg tgcctttgtt
     1921 cactggtacg ttggggaggg gatggaggaa ggtgagtttt cagaggcccg tgaggacatg
     1981 gctgcccttg agaaggatta tgaggaggtt ggtgtggatt ctgttgaagg agagggtgag
     2041 gaagaaggag aggaatacct cgagtaccca tacgacgtac ctgattatgc aacttatcct
     2101 tatgatgtac ctgattatgc tagctatcct tatgatgtcc cagactacgc atagtgaggg
     2161 cccgatatct cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     2221 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     2281 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     2341 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     2401 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     2461 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     2521 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     2581 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     2641 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     2701 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     2761 cgcctggaaa cgggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     2821 cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcata
     2881 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
     2941 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
     3001 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcagatctg cgcagctggg
     3061 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3121 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     3181 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggcatcc
     3241 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     3301 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     3361 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     3421 tctattcttt tgatttataa gggattttgg ggatttcggc ctattggtta aaaaatgagc
     3481 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     3541 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3601 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3661 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     3721 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     3781 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     3841 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     3901 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     3961 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     4021 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     4081 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     4141 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     4201 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     4261 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     4321 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     4381 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     4441 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     4501 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     4561 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     4621 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     4681 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgc gaaatgaccg
     4741 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     4801 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     4861 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     4921 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4981 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     5041 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     5101 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     5161 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     5221 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     5281 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     5341 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     5401 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     5461 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     5521 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     5581 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     5641 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     5701 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     5761 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     5821 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     5881 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     5941 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     6001 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6061 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     6121 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     6181 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6241 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6301 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6361 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6421 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6481 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     6541 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6601 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6661 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6721 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6781 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     6841 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6901 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     6961 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7021 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7081 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7141 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     7201 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtcga ctctcagtac
     7261 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
     7321 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
     7381 catgaagaat ctgcttagg
//
